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| <section id="Content"> | | <section id="Content"> |
| <h1>Protocols</h1> | | <h1>Protocols</h1> |
- | <h2>General Protocols</h2>
| + | <p>Enter Text Here</p> |
- | <ul>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/BacterialTransformation" target="_blank">Bacterial Transformation</a></li>
| + | </section> |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GlycerolStocks" target="_blank">Glycerol Stocks</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/LBAgarPlates" target="_blank">LB Agar Plates</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/MakingChemicallyCompetentEcoliCells" target="_blank">Making Chemically Competent <i>E. coli</i> Cells</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/OvernightCultures" target="_blank">Overnight Cultures</a></li>
| + | |
- | </ul>
| + | |
- | <h2>DNA Protocols</h2>
| + | |
- | <ul>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/AgaroseGelElectrophoresis" target="_blank">Agarose Gel Electrophoresis</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/ColonyPCR" target="_blank">Colony PCR</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GelExtraction" target="_blank">Gel Extraction</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GibsonAssembly" target="_blank">Gibson Assembly</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/KapaPCR" target="_blank">Kapa PCR</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PCRPurification" target="_blank">PCR Purification</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PlasmidPurificationfromEcoli" target="_blank">Plasmid Purification from E. coli</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/RehydrationOfRegistryDNA" target="_blank">Rehydration of Registry DNA</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/RestrictionDigestionAntarcticPhosphataseAndLigation" target="_blank">Restriction Digestion, Antarctic Phosphatase, & Ligation</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/VerificationDigest" target="_blank">Verification Digest</a></li>
| + | |
- | </ul>
| + | |
- | <h2>Protein Protocols</h2>
| + | |
- | <ul>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/BradfordAssay" target="_blank">Bradford Assay</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/FreezeThawProtocolforProteinSamples" target="_blank">Freeze-Thaw Protocol for Protein Samples</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/Immunoprecipitation" target="_blank">Immunoprecipitation</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/LargeScaleProteinExpressionAndPurification" target="_blank">Large Scale Protein Expression & Purification</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/MiniExpression" target="_blank">Mini Expression</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/NativePAGEGel" target="_blank">Native PAGE Gel</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/OsmoticShock" target="_blank">Osmotic Shock</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/SDSPAGEGel" target="_blank">SDS PAGE Gel</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/WesternBlot" target="_blank">Western Blot</a></li>
| + | |
- | </ul>
| + | |
- | <h2>TALE Protocols</h2>
| + | |
- | <ul>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/CaptureAssay" target="_blank">Capture Assay</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/FunctionalityAssayOnNitrocellulose" target="_blank">Functionality Assay on Nitrocellulose</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GelShiftAssay" target="_blank">Gel Shift Assay</a></li>
| + | |
- | </ul>
| + | |
- | <h2>Ferritin Protocols</h2>
| + | |
- | <ul>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/NitrocelluloseAssay" target="_blank">Nitrocellulose Assay</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PrussianBlueFerritinMichaelisMentenKineticAnalysis" target="_blank">Prussian Blue Ferritin Michaelis-Menten Kinetic Analysis</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PrussianBlueFerritinPHOptimization" target="_blank">Prussian Blue Ferritin pH Optimization</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PrussianBlueFerritinSynthesis" target="_blank">Prussian Blue Ferritin Synthesis</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PrussianBlueFerritinTemperatureOptimization" target="_blank">Prussian Blue Ferritin Temperature Optimization</a></li>
| + | |
- | </ul>
| + | |
- | <h2>β-Lactamase Protocols</h2>
| + | |
- | <ul>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/AmpicillinSurvivalAssay1" target="_blank">Ampicillin Survival Assay 1</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/AmpicillinSurvivalAssay2" target="_blank">Ampicillin Survival Assay 2</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/BenzylpenicillianAssay" target="_blank">Benzylpenicillin Assay</a></li>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/BenzylpenicillianAssay2" target="_blank">Benzylpenicillin Assay 2</a></li>
| + | |
- | </ul>
| + | |
- | <h2>Scaffold Protocols</h2>
| + | |
- | <ul>
| + | |
- | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/CoilBindingNitrocelluloseAssay" target="_blank">Coil Binding Nitrocellulose Assay</a></li>
| + | |
- | </ul>
| + | |
- | </section>
| + | |
| </html> | | </html> |