Team:TU-Munich/Results/Software

From 2013.igem.org

(Difference between revisions)
(→Export of the Computed Parameters)
(→Export of the Computed Parameters)
Line 121: Line 121:
|colspan="17"|This is a RFC 25 BioBrick, thus ATGGCCGGC and ACCGGT were added to the 5' and 3' ends.
|colspan="17"|This is a RFC 25 BioBrick, thus ATGGCCGGC and ACCGGT were added to the 5' and 3' ends.
|-
|-
-
|colspan="20"|AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
|colspan="20"|AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
|-
|-
|colspan="20"| The BioBrick-AutoAnnotator was created by [https://2013.igem.org/Team:TU-Munich TU-Munich 2013] iGEM team. Software License??? For information please read the [https://2013.igem.org/Team:TU-Munich/Results/Software description].
|colspan="20"| The BioBrick-AutoAnnotator was created by [https://2013.igem.org/Team:TU-Munich TU-Munich 2013] iGEM team. Software License??? For information please read the [https://2013.igem.org/Team:TU-Munich/Results/Software description].

Revision as of 13:08, 25 July 2013


The AutoAnnotator

Introduction to the Idea behind our AutoAnnotator

The parts registry contains a wide range of interesting BioBricks from which many have similarity: they are not perfectly described and annotated. This is a real pity because after the identification of the open reading fram a multitude of parameters of the protein can be computed automatically. We are developing a tool which is able to identify the open reading frame of a BioBrick, analyze it for different parameters and export the results in a format that can easily be importet into a part description a a single table.
Figure on the steps.

Import of BioBrick Sequences

Text
figure




Determination of the Open Reading Frame

Text
figure




Analysis of Parameters

Text
figure




Export of the Computed Parameters

Text


figure


Automatically determined parameters using the [http://www.mediawiki.org/wiki/Table BioBrick-AutoAnnotator]
BioBrick: <partinfo>BBa_K1159000</partinfo> Used open reading frame from position ?x? to ?y?.
A (Ala) ?4 R (Arg) ?4 N (Asn) ?4 D (Asp) ?4? C (Cys) ?4 Q (Gln) ?4 E (Glu) ?4 G (Gly) ?4 H (His) ?4?
K (Lys): ?4 M (Met) ?4 F (Phe) ?4 P (Pro) ?4 S (Ser) ?4 T (Thr) ?4 W (Trp) ?4 Y (Tyr) ?4 V (Val) ?4
Amino acid counting: Total number of amino acids (aa): ?99? Number of positively charged aa: ?9? Number of negatively charged aa: ?9?
Biochemical parameters: Molecular weight [Da]: ?4? Theoretical pI: ?4? Extinction coefficient: ?4?
Estimated half-life: Mammals: ?4 h? Yeast: ?4? E. coli: ?4?
Codon usage: Mammals: ?good? Yeast: ?Ok? E. coli: ?bad ?
RFC standard: This is a RFC 25 BioBrick, thus ATGGCCGGC and ACCGGT were added to the 5' and 3' ends.
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. Software License??? For information please read the description.



References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.



$(document).ready(function(){


// put the footer in the right place

$("#footer-box").prepend($("#social-footer"));


// implement image preloading

var images = new Array()

function preload() { 

   for (i = 0; i < preload.arguments.length; i++) {
       images[i] = new Image()
       images[i].src = preload.arguments[i]
   }

}


// preload menu backgrounds

preload( "TUM13_menu-highlight.png", 

        "TUM13_submenu-bg.png",
        "TUM13_submenu-highlight.png",
        "TUM13_tour-start-highlight.png",
        "TUM13_tour-previous-highlight.png",
        "TUM13_tour-next-highlight.png" );

// preload team pictures

if ( $("div#teamfield").length > 0 ) { preload( "TUM13_raven-front.png", 

        "TUM13_raven-top.png",
        "TUM13_raven-top-left.png",
        "TUM13_raven-left.png",
        "TUM13_raven-bottom-left.png",
        "TUM13_raven-bottom.png",
        "TUM13_raven-bottom-right.png",
        "TUM13_raven-right.png",
        "TUM13_raven-top-right.png",
        "TUM13_katrin-front.png",        // Katrin
        "TUM13_katrin-t.png",
        "TUM13_katrin-t-l.png",
        "TUM13_katrin-l.png",
        "TUM13_katrin-b-l.png",
        "TUM13_katrin-b.png",
        "TUM13_katrin-b-r.png",
        "TUM13_katrin-r.png",
        "TUM13_katrin-t-r.png",
        "TUM13_rosario-front.png",        // Rosario
        "TUM13_rosario-t.png",
        "TUM13_rosario-t-l.png",
        "TUM13_rosario-l.png",
        "TUM13_rosario-b-l.png",
        "TUM13_rosario-b.png",
        "TUM13_rosario-b-r.png",
        "TUM13_rosario-r.png",
        "TUM13_rosario-t-r.png",
        "TUM13_fabian-front.png",        // Fabian
        "TUM13_fabian-t.png",
        "TUM13_fabian-t-l.png",
        "TUM13_fabian-l.png",
        "TUM13_fabian-b-l.png",
        "TUM13_fabian-b.png",
        "TUM13_fabian-b-r.png",
        "TUM13_fabian-r.png",
        "TUM13_fabian-t-r.png",
        "TUM13_andreas-front.png",        // Andreas
        "TUM13_andreas-t.png",
        "TUM13_andreas-t-l.png",
        "TUM13_andreas-l.png",
        "TUM13_andreas-b-l.png",
        "TUM13_andreas-b.png",
        "TUM13_andreas-b-r.png",
        "TUM13_andreas-r.png",
        "TUM13_andreas-t-r.png",
        "TUM13_louise-front.png",        // Louise
        "TUM13_louise-t.png",
        "TUM13_louise-t-l.png",
        "TUM13_louise-l.png",
        "TUM13_louise-b-l.png",
        "TUM13_louise-b.png",
        "TUM13_louise-b-r.png",
        "TUM13_louise-r.png",
        "TUM13_louise-t-r.png",
        "TUM13_johanna-front.png",        // Johanna
        "TUM13_johanna-t.png",
        "TUM13_johanna-t-l.png",
        "TUM13_johanna-l.png",
        "TUM13_johanna-b-l.png",
        "TUM13_johanna-b.png",
        "TUM13_johanna-b-r.png",
        "TUM13_johanna-r.png",
        "TUM13_johanna-t-r.png",
        "TUM13_meike-front.png",        // Meike
        "TUM13_meike-t.png",
        "TUM13_meike-t-l.png",
        "TUM13_meike-l.png",
        "TUM13_meike-b-l.png",
        "TUM13_meike-b.png",
        "TUM13_meike-b-r.png",
        "TUM13_meike-r.png",
        "TUM13_meike-t-r.png",
        "TUM13_volker-front.png",        // Volker
        "TUM13_volker-t.png",
        "TUM13_volker-t-l.png",
        "TUM13_volker-l.png",
        "TUM13_volker-b-l.png",
        "TUM13_volker-b.png",
        "TUM13_volker-b-r.png",
        "TUM13_volker-r.png",
        "TUM13_volker-t-r.png",
        "TUM13_polte-front.png",        // Polte
        "TUM13_polte-t.png",
        "TUM13_polte-t-l.png",
        "TUM13_polte-l.png",
        "TUM13_polte-b-l.png",
        "TUM13_polte-b.png",
        "TUM13_polte-b-r.png",
        "TUM13_polte-r.png",
        "TUM13_polte-t-r.png",
        "TUM13_leonie-front.png",        // Leonie
        "TUM13_leonie-t.png",
        "TUM13_leonie-t-l.png",
        "TUM13_leonie-l.png",
        "TUM13_leonie-b-l.png",
        "TUM13_leonie-b.png",
        "TUM13_leonie-b-r.png",
        "TUM13_leonie-r.png",
        "TUM13_leonie-t-r.png",
        "TUM13_philipp-front.png",        // Philipp
        "TUM13_philipp-t.png",
        "TUM13_philipp-t-l.png",
        "TUM13_philipp-l.png",
        "TUM13_philipp-b-l.png",
        "TUM13_philipp-b.png",
        "TUM13_philipp-b-r.png",
        "TUM13_philipp-r.png",
        "TUM13_philipp-t-r.png",
        "TUM13_jeff-front.png",        // Jeff
        "TUM13_jeff-t.png",
        "TUM13_jeff-t-l.png",
        "TUM13_jeff-l.png",
        "TUM13_jeff-b-l.png",
        "TUM13_jeff-b.png",
        "TUM13_jeff-b-r.png",
        "TUM13_jeff-r.png",
        "TUM13_jeff-t-r.png",
        "TUM13_chris-front.png",        // Chris
        "TUM13_chris-t.png",
        "TUM13_chris-t-l.png",
        "TUM13_chris-l.png",
        "TUM13_chris-b-l.png",
        "TUM13_chris-b.png",
        "TUM13_chris-b-r.png",
        "TUM13_chris-r.png",
        "TUM13_chris-t-r.png",
        "TUM13_flo-front.png",        // Flo
        "TUM13_flo-t.png",
        "TUM13_flo-t-l.png",
        "TUM13_flo-l.png",
        "TUM13_flo-b-l.png",
        "TUM13_flo-b.png",
        "TUM13_flo-b-r.png",
        "TUM13_flo-r.png",
        "TUM13_flo-t-r.png" );

}

// Slideshows

$('.bxslider').bxSlider({ 

   responsive: false,
   auto: true,
   autoHover: true

});

$('.bxgallery').bxSlider({ 

   slideMargin: 10,
   minSlides: 3,
   maxSlides: 3,
   moveSlides: 1,
   slideWidth: 5000

});

$("ul.bxgallery img").slimbox({ loop: true }, function(el) { url = el.src; url = url.substring(0, url.lastIndexOf('/')); url = url.replace('/thumb/', '/'); // description = $(el).parents("div.thumbinner").children("div.thumbcaption").text(); return [url, ]; }, function(el) { return (this == el) || (this.parentNode.parentNode && (this.parentNode.parentNode == el.parentNode.parentNode)); });


// Counter and Countdown

function render_counter(c) { i = 0; iid = window.setInterval(function(){ if ( (c-i) > (c/200) ) { $('span#counter').html(i); i += Math.round(c/200); } else { $('span#counter').html(c); window.clearInterval(iid); } }, 10); }

if ($('span#counter').length > 0) { $.ajax({ url: "https://2013.igem.org/Special:PopularPages", success: function( html ) { dom = $.parseHTML(html); visitors = $(dom).find('a[title="Team:TU-Munich"]').parent().text(); visitors = visitors.substring(visitors.indexOf('(')+1); visitors = visitors.substring(0, visitors.indexOf(' ')); visitors = visitors.replace(',', ); render_counter(visitors); }, error: function( xhr, status ) { render_counter(4700); } }); }

if ($('span#countdown) { clock = window.setInterval(function(){ jetzt = new Date(); time_left = Date.UTC(2013, 9, 5, 4, 0, 0) - Date.UTC(jetzt.getUTCFullYear(), jetzt.getUTCMonth(), jetzt.getUTCDate(), jetzt.getUTCHours(), jetzt.getUTCMinutes(), jetzt.getUTCSeconds()); left_sec = (time_left/1000)%60; left_sec = (left_sec < 10) ? "0" + left_sec : left_sec; left_min = Math.floor(time_left/60000)%60; left_min = (left_min < 10) ? "0" + left_min : left_min; left_h = Math.floor(time_left/3600000)%24; left_h = (left_h < 10) ? "0" + left_h : left_h; left_d = Math.floor(time_left/86400000); left_d = (left_d == 1) ? left_d + " day" : left_d + " days"; $('span#countdown').html(left_d + " " + left_h + ":" + left_min + ":" + left_sec); }, 1000); }

// Animate teamfield

if ( $("div#teamfield").length > 0 ) {

var $members = $("div#teamfield a");

$("body").mousemove(function(event){ for (i=0; i<$members.length; i++) {

if ( $members.eq(i).offset().left > event.pageX ) { 

   if ( $members.eq(i).offset().top > event.pageY ) {

       $members.eq(i).removeClass();
       $members.eq(i).addClass("top-left");

   } else if ( $members.eq(i).offset().top <= event.pageY && ( $members.eq(i).offset().top + $members.eq(i).height() ) >= event.pageY ) {

       $members.eq(i).removeClass();
       $members.eq(i).addClass("left");

   } else if ( ( $members.eq(i).offset().top + $members.eq(i).height() ) < event.pageY ) {

       $members.eq(i).removeClass();
       $members.eq(i).addClass("bottom-left");

   }

} else if ( $members.eq(i).offset().left <= event.pageX && ( $members.eq(i).offset().left + $members.eq(i).width() ) >= event.pageX ) { 

   if ( $members.eq(i).offset().top > event.pageY ) {

       $members.eq(i).removeClass();
       $members.eq(i).addClass("top");

   } else if ( $members.eq(i).offset().top <= event.pageY && ( $members.eq(i).offset().top + $members.eq(i).height() ) >= event.pageY ) {

       $members.eq(i).removeClass();
       $members.eq(i).addClass("front");

   } else if ( ( $members.eq(i).offset().top + $members.eq(i).height() ) < event.pageY ) {

       $members.eq(i).removeClass();
       $members.eq(i).addClass("bottom");

   }

} else if ( ( $members.eq(i).offset().left + $members.eq(i).width() ) < event.pageX ) { 

   if ( $members.eq(i).offset().top > event.pageY ) {

       $members.eq(i).removeClass();
       $members.eq(i).addClass("top-right");

   } else if ( $members.eq(i).offset().top <= event.pageY && ( $members.eq(i).offset().top + $members.eq(i).height() ) >= event.pageY ) {

       $members.eq(i).removeClass();
       $members.eq(i).addClass("right");

   } else if ( ( $members.eq(i).offset().top + $members.eq(i).height() ) < event.pageY ) {

       $members.eq(i).removeClass();
       $members.eq(i).addClass("bottom-right");

   }

}

} });

}

});

Retrieved from "http://2013.igem.org/Team:TU-Munich/Results/Software"