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Revision as of 22:47, 6 August 2013 (view source) Sgrenz (Talk | contribs) ← Older edit		Revision as of 22:50, 6 August 2013 (view source) Sgrenz (Talk | contribs) Newer edit →
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	<table border=1 style="float:left; margin-left:20px">		<table border=1 style="float:left; margin-left:20px">
-	<tr><td>asdas</td></tr>	+	<tr><th>Program Phusion</th></tr>
		+	<tr><td>98°C</td><td>98°C</td><td>70°C</td><td>72°C</td><td>72°C</td></tr>
		+	<tr><td>2min</td> <td>30s</td><td>30s</td> <td>45s</td><td>6min</td></tr>
	</table>		</table>
	</html>		</html>

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Revision as of 22:50, 6 August 2013

PCR Protocols and Programs

Phusion 25 µl

Reagenz	1x	8x
Buffer 5x	5,0	44
MgSO4	0	0
dNTPs	2,5	22
Primer1	1,0	8,8
Primer2	1,0	8,8
Template	1,5	13,2
DMSO	2,0	17,6
Polymerase	0,5	4,4
H2O	11,5	101,2

Program Phusion
98°C	98°C	70°C	72°C	72°C
2min	30s	30s	45s	6min