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Team:Evry/Protocols/07
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<h3> Principle</h3> | <h3> Principle</h3> | ||
| + | Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence. <br> | ||
| + | PCR is divided into 5 steps: | ||
| + | First denaturation | ||
| + | Denaturation step | ||
| + | Anealing step | ||
| + | Elongation step | ||
| + | Final step | ||
| + | The 2nd, 3th and 4th steps are repeated 20-40 cycles. | ||
| + | |||
| + | |||
| + | |||
<h3> Preparation</h3> | <h3> Preparation</h3> | ||
<h3> Optimisation</h3> | <h3> Optimisation</h3> | ||
Revision as of 10:31, 21 August 2013
PCR and gel electrophoresis analysis
PCR
Principle
Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence.PCR is divided into 5 steps: First denaturation Denaturation step Anealing step Elongation step Final step The 2nd, 3th and 4th steps are repeated 20-40 cycles.
Preparation
Optimisation
Number of primer
Temperature
Gel electrophoresis analysis
Principle
Preparation
Analysis
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