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Team:Evry/Protocols/07
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<h3> Principle</h3> | <h3> Principle</h3> | ||
Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence. <br> | Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence. <br> | ||
| - | PCR is divided into 5 steps: | + | PCR is divided into 5 steps:<br> |
| - | First denaturation | + | First denaturation<br> |
| - | Denaturation step | + | Denaturation step<br> |
| - | Anealing step | + | Anealing step<br> |
| - | Elongation step | + | Elongation step<br> |
| - | Final step | + | Final step<br> |
| - | The 2nd, 3th and 4th steps are repeated 20-40 cycles. | + | The 2nd, 3th and 4th steps are repeated 20-40 cycles.<br> |
Revision as of 10:32, 21 August 2013
PCR and gel electrophoresis analysis
PCR
Principle
Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence.PCR is divided into 5 steps:
First denaturation
Denaturation step
Anealing step
Elongation step
Final step
The 2nd, 3th and 4th steps are repeated 20-40 cycles.
Preparation
Optimisation
Number of primer
Temperature
Gel electrophoresis analysis
Principle
Preparation
Analysis
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