"
Team:Evry/Protocols/07
From 2013.igem.org
(Difference between revisions)
| Line 18: | Line 18: | ||
<li>Anealing step<br> | <li>Anealing step<br> | ||
<li>Elongation step<br> | <li>Elongation step<br> | ||
| - | <li>Final step<br></ul> | + | <li>Final step<br></ul><br> |
The 2nd, 3th and 4th steps are repeated 20-40 cycles.<br></p> | The 2nd, 3th and 4th steps are repeated 20-40 cycles.<br></p> | ||
Revision as of 12:13, 21 August 2013
PCR and gel electrophoresis analysis
PCR
Principle
Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence.
PCR is divided into 5 steps:
- First denaturation
- Denaturation step
- Anealing step
- Elongation step
- Final step
The 2nd, 3th and 4th steps are repeated 20-40 cycles.
Preparation
Optimisation
Number of primer
Temperature
Gel electrophoresis analysis
Principle
Preparation
Analysis
Site developped by Gabriel Guillocheau
