Team:TU-Munich/Results/GM-Moss
From 2013.igem.org
(→Transgenic Physcomitrella patnes plants) |
(→Transgenic Physcomitrella patnes plants) |
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|- | |- | ||
|PF-10 | |PF-10 | ||
- | |Stressinducible_Promoter-RFP ( | + | |Stressinducible_Promoter-RFP (Stress) |
|<partinfo>BBa_E0040</partinfo> | |<partinfo>BBa_E0040</partinfo> | ||
| | | | ||
Line 113: | Line 113: | ||
|- | |- | ||
|PF-11 | |PF-11 | ||
- | |SpyCatcher-SERK_Receptor:Spytag-nLuc | + | |SpyCatcher-SERK_Receptor:Spytag-nLuc (Catcher:Tag-nLuc) |
|<partinfo>BBa_E0040</partinfo> | |<partinfo>BBa_E0040</partinfo> | ||
| | | | ||
Line 120: | Line 120: | ||
|- | |- | ||
|PF-12 | |PF-12 | ||
- | |SpyCatcher-SERK_Receptor:nLuc-Spytag | + | |SpyCatcher-SERK_Receptor:nLuc-Spytag (Catcher:nLuc-Tag |
|<partinfo>BBa_E0040</partinfo> | |<partinfo>BBa_E0040</partinfo> | ||
| | | | ||
Line 127: | Line 127: | ||
|- | |- | ||
|PF-13 | |PF-13 | ||
- | |SpyTag-SERK_Receptor:SpyCatcher-nLuc | + | |SpyTag-SERK_Receptor:SpyCatcher-nLuc (Tag:Catcher-nLuc) |
|<partinfo>BBa_E0040</partinfo> | |<partinfo>BBa_E0040</partinfo> | ||
| | | | ||
Line 134: | Line 134: | ||
|- | |- | ||
|PF-14 | |PF-14 | ||
- | |SpyTag-SERK_Receptor:nLuc-SpyCatcher | + | |SpyTag-SERK_Receptor:nLuc-SpyCatcher (Tag:nLuc-Catcher) |
|<partinfo>BBa_E0040</partinfo> | |<partinfo>BBa_E0040</partinfo> | ||
| | | | ||
Line 141: | Line 141: | ||
|- | |- | ||
|PF-15 | |PF-15 | ||
- | | | + | |Alcohol acetyltransferase I (Banana) |
- | |<partinfo> | + | |<partinfo>BBa_J45014</partinfo> |
| | | | ||
| | | | ||
Line 148: | Line 148: | ||
|- | |- | ||
|PF-16 | |PF-16 | ||
- | | | + | |Kill-switch with PIF3 (PIF-3) |
|<partinfo>BBa_E0040</partinfo> | |<partinfo>BBa_E0040</partinfo> | ||
| | | | ||
Line 155: | Line 155: | ||
|- | |- | ||
|PF-17 | |PF-17 | ||
- | | | + | |Kill-switch with PIF6 (PIF-6) |
|<partinfo>BBa_E0040</partinfo> | |<partinfo>BBa_E0040</partinfo> | ||
| | | | ||
Line 162: | Line 162: | ||
|- | |- | ||
|PF-18 | |PF-18 | ||
- | | | + | |xx (FRET) |
|<partinfo>BBa_E0040</partinfo> | |<partinfo>BBa_E0040</partinfo> | ||
| | | |
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xxx
Transgenic Physcomitrella patnes plants created
Table 1:
Transgenic Physcomitrella patnes plants | |||||
Number | Construct | BioBrick | Figure | Size [bp] | Lane |
PF-1 | GFP (GFP-cyt) | <partinfo>BBa_E0040</partinfo> | |||
PF-2 | Igk-GFP (GFP-sec) | <partinfo>BBa_E0040</partinfo> | |||
PF-3 | NanoLuciferase (nLuc-cyt) | <partinfo>BBa_E0040</partinfo> | |||
PF-4 | Igk-NanoLuciferase (nLuc-sec) | <partinfo>BBa_E0040</partinfo> | |||
PF-5 | NanoLuciferase-SERK_Receptor (nLuc-rec) | <partinfo>BBa_E0040</partinfo> | |||
PF-6 | Erythromycinesterase (EreB-cyt) | <partinfo>BBa_E0040</partinfo> | |||
PF-7 | Erythromycinesterase-SERK_Receptor (EreB-rec) | <partinfo>BBa_E0040</partinfo> | |||
PF-6 | Catecholdioxygenase (XylE-cyt) | <partinfo>BBa_E0040</partinfo> | |||
PF-7 | DDT-dehydrochlorinase (GST-cyt) | <partinfo>BBa_E0040</partinfo> | |||
PF-8 | PP1-SERK_Receptor (PP1-rec) | <partinfo>BBa_E0040</partinfo> | |||
PF-9 | FluA-SERK_Receptor (FluA-rec) | <partinfo>BBa_E0040</partinfo> | |||
PF-10 | Stressinducible_Promoter-RFP (Stress) | <partinfo>BBa_E0040</partinfo> | |||
PF-11 | SpyCatcher-SERK_Receptor:Spytag-nLuc (Catcher:Tag-nLuc) | <partinfo>BBa_E0040</partinfo> | |||
PF-12 | SpyCatcher-SERK_Receptor:nLuc-Spytag (Catcher:nLuc-Tag | <partinfo>BBa_E0040</partinfo> | |||
PF-13 | SpyTag-SERK_Receptor:SpyCatcher-nLuc (Tag:Catcher-nLuc) | <partinfo>BBa_E0040</partinfo> | |||
PF-14 | SpyTag-SERK_Receptor:nLuc-SpyCatcher (Tag:nLuc-Catcher) | <partinfo>BBa_E0040</partinfo> | |||
PF-15 | Alcohol acetyltransferase I (Banana) | <partinfo>BBa_J45014</partinfo> | |||
PF-16 | Kill-switch with PIF3 (PIF-3) | <partinfo>BBa_E0040</partinfo> | |||
PF-17 | Kill-switch with PIF6 (PIF-6) | <partinfo>BBa_E0040</partinfo> | |||
PF-18 | xx (FRET) | <partinfo>BBa_E0040</partinfo> | |||
PF-19 | <partinfo>BBa_E0040</partinfo> | ||||
PF-20 | <partinfo>BBa_E0040</partinfo> |
References:
http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984
- http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.
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