Team:Evry/Protocols/07

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<h1> PCR and gel electrophoresis analysis </h1>
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<h1> PCR </h1>
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<h2> PCR</h2>
 
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<h3> Principle</h3>
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<h2> Principle</h2>
<p>Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence. <br>
<p>Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence. <br>
PCR is divided into 5 steps:<br>
PCR is divided into 5 steps:<br>
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<h3> Preparation</h3>
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<h2> Preparation</h2>
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<h3> Optimisation</h3>
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<h2> Optimisation</h2>
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<h4> Number of primer</h4>
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<h3> Number of primer</h3>
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<h4> Temperature</h4>
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<h3> Temperature</h3>
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<h2> Gel electrophoresis analysis</h2>
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<h1> Gel electrophoresis analysis</h1>
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<h3> Principle</h3>
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<h2> Principle</h2>
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<h3> Preparation</h3>
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<h2> Preparation</h2>
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<h3> Analysis</h3>
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<h2> Analysis</h2>

Revision as of 11:40, 26 August 2013

Iron coli project

PCR

Principle

Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence.
PCR is divided into 5 steps:
First denaturation
Denaturation step
Anealing step
Elongation step
Final step
The 2nd, 3th and 4th steps are repeated 20-40 cycles.

Denaturation step

Preparation

Optimisation

Number of primer

Temperature

Gel electrophoresis analysis

Principle

Preparation

Analysis

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