Team:Evry/Protocols/11

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<i>Protocol adapted from Thermo Scientific PCR Purification notebook</i><br><br>
<i>Protocol adapted from Thermo Scientific PCR Purification notebook</i><br><br>
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<h2> Goal </h2>
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<p>The aim of the purification step is to recover the plasmid produced by the bacteria (cloning strain).<br>
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The different step are use to throw other bacterial components off (proteins, cell wall, genomic DNA, etc).</p>
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<h2> Preparation </h2>
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 +
<i>Protocol adapted from Macherey-Nagel plasmid purification notebook</i><br><br>
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<b><p>1. </b><br>
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<br><br></p>
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<p><b>2. </b><br>
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<br><br><p>
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<p><b>3. </b> <br>
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 +
<br>
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<br>
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<br><br></p>
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<p><b>4. </b><br>
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<br><br></p>
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<p><b>5. <br></b>
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<br><br></p>
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<p><b>6. <br></b>
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<br><br></p>
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<p><b>7. <br></b>
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<br><br></p>
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<p><b>8. <br></b>
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<br></p>
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<h2> Test </h2>
<b><p>
<b><p>

Revision as of 12:56, 29 August 2013

Iron coli project

PCR Purification

Aim

Preparation

Protocol adapted from Thermo Scientific PCR Purification notebook

Goal

The aim of the purification step is to recover the plasmid produced by the bacteria (cloning strain).
The different step are use to throw other bacterial components off (proteins, cell wall, genomic DNA, etc).

Preparation

Protocol adapted from Macherey-Nagel plasmid purification notebook

1.


2.


3.




4.


5.


6.


7.


8.

Test

Retrieved from "http://2013.igem.org/Team:Evry/Protocols/11"