Team:Evry/Protocols/11

From 2013.igem.org

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<h2> Goal </h2>
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<p>The aim of the PCRpurification step is to recover DNA from PCR and other enzymatic reacti mixtures.</p>
<h2> Preparation </h2>
<h2> Preparation </h2>
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<h2> Goal </h2>
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<p>The aim of the purification step is to recover the plasmid produced by the bacteria (cloning strain).<br>
 
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The different step are use to throw other bacterial components off (proteins, cell wall, genomic DNA, etc).</p>
 
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<h2> Preparation </h2>
 
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<i>Protocol adapted from Macherey-Nagel plasmid purification notebook</i><br><br>
 

Revision as of 13:04, 29 August 2013

Iron coli project

PCR Purification

Goal

The aim of the PCRpurification step is to recover DNA from PCR and other enzymatic reacti mixtures.

Preparation

Protocol adapted from Thermo Scientific PCR Purification notebook

Goal

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Test

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