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Team:Bielefeld-Germany/Labjournal/July
From 2013.igem.org
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<h2><a name=July>July</a></h2> | <h2><a name=July>July</a></h2> | ||
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<h4><a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Cytochromes">Cytochromes</a></h4> | <h4><a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Cytochromes">Cytochromes</a></h4> | ||
| - | <p></p> | + | <div class="cyt_primer_hl"><p><a>Design and order of new Primer</a></p></div> |
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| + | The mtrCAB fragment has two illegal PstI restriction sites at x xbp and yy bp, so we had to design new primers to remove them. We replaced one base in each restriction site, without affecting the coding triplett, by respective primer overlaps . We ended up with three different fragments, which will be ligated back together via Gibson Assembly. | ||
| + | <br>mtrCAB_Frag1_rev: | ||
| + | <br>mtrCAB_Frag2_fwd: | ||
| + | <br>mtrCAB_Frag2_rev: | ||
| + | <br>mtrCAB_Frag3_fwd: | ||
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| + | </blockquote> | ||
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<h4><a href="https://2013.igem.org/Team:Bielefeld-Germany/BioSafety">Biosafety</a></h4> | <h4><a href="https://2013.igem.org/Team:Bielefeld-Germany/BioSafety">Biosafety</a></h4> | ||
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Revision as of 14:08, 2 September 2013
July
Milestones
1.Week
MFC
Mediators
Cytochromes
The mtrCAB fragment has two illegal PstI restriction sites at x xbp and yy bp, so we had to design new primers to remove them. We replaced one base in each restriction site, without affecting the coding triplett, by respective primer overlaps . We ended up with three different fragments, which will be ligated back together via Gibson Assembly.
mtrCAB_Frag1_rev:
mtrCAB_Frag2_fwd:
mtrCAB_Frag2_rev:
mtrCAB_Frag3_fwd:
