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Team:Bielefeld-Germany/Labjournal/July
From 2013.igem.org
(Difference between revisions)
| Line 66: | Line 66: | ||
<div class="cyt_frag1"> | <div class="cyt_frag1"> | ||
<blockquote> | <blockquote> | ||
| - | + | Size:1800 bp | |
<br>Program: <a href="https://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#cyt_frag1">PhusionPCR</a> | <br>Program: <a href="https://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#cyt_frag1">PhusionPCR</a> | ||
<br>mtrC_fwd: | <br>mtrC_fwd: | ||
| Line 76: | Line 76: | ||
<div class="cyt_frag2"> | <div class="cyt_frag2"> | ||
<blockquote> | <blockquote> | ||
| - | + | Size:330 bp | |
<br>Program: <a href="https://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#cyt_frag2">PhusionPCR</a> | <br>Program: <a href="https://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#cyt_frag2">PhusionPCR</a> | ||
<br>mtr_Frag2_fwd: | <br>mtr_Frag2_fwd: | ||
| Line 86: | Line 86: | ||
<div class="cyt_frag3"> | <div class="cyt_frag3"> | ||
<blockquote> | <blockquote> | ||
| - | + | Size:3000 bp | |
<br>Program: <a href="https://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#cyt_frag3">PhusionPCR</a> | <br>Program: <a href="https://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#cyt_frag3">PhusionPCR</a> | ||
<br>mtr_Frag3_fwd: | <br>mtr_Frag3_fwd: | ||
Revision as of 14:59, 2 September 2013
July
Milestones
1.Week
MFC
Mediators
Cytochromes
The mtrCAB fragment has two illegal PstI restriction sites at x xbp and yy bp, so we had to design new primers to remove them. We replaced one base in each restriction site, without affecting the coding triplett, by respective primer overlaps . We ended up with three different fragments, which will be ligated back together via Gibson Assembly.
mtrCAB_Frag1_rev:
mtrCAB_Frag2_fwd:
mtrCAB_Frag2_rev:
mtrCAB_Frag3_fwd:
Size:1800 bp
Program: PhusionPCR
mtrC_fwd:
mtr_Frag1_rev:
Size:330 bp
Program: PhusionPCR
mtr_Frag2_fwd:
mtr_Frag2_rev:
Size:3000 bp
Program: PhusionPCR
mtr_Frag3_fwd:
mtr_B_rev:
