Team:Uppsala/test

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<title>Uppsala iGEM 2013</title>
<title>Uppsala iGEM 2013</title>
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<div id="igem_logo"><a href="https://2013.igem.org/Main_Page"><img class="igem_logo" src="https://static.igem.org/mediawiki/2013/e/e0/Uppsala2013_IGEMlogo.png"></a></div>
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<div id="igem_logo"><a href="https://2013.igem.org/Team:Uppsala"><img class="igem_logo" src="https://static.igem.org/mediawiki/2013/e/e0/Uppsala2013_IGEMlogo.png"></a></div>
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<li><a href="https://2013.igem.org/Team:Uppsala/project" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/a/aa/Uppsala2013_Project.png"></a>
<li><a href="https://2013.igem.org/Team:Uppsala/project" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/a/aa/Uppsala2013_Project.png"></a>
<ul>
<ul>
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<li><a href="https://2013.igem.org/Team:Uppsala/overview">Overview</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/chassi">Chassi</a>
<li><a href="https://2013.igem.org/Team:Uppsala/chassi">Chassi</a>
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<ul>
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<li><a href="https://2013.igem.org/Team:Uppsala/promoters">Promoters</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/promoters">Promoters</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/reporter-genes">Reporter genes</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/reporter-genes">Reporter genes</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/affinity-tags">Toxin-antitoxin system</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/toxin-antitoxin-system">Toxin-antitoxin system</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/vectors">Vectors</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/vectors">Vectors</a></li>
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/signal-peptide">Signal peptide</a></li>
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/signal-peptide">Signal peptide</a></li>
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                                                 <li><a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering">Metabolic engineering</a>
                                                 <li><a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering">Metabolic engineering</a>
                                                     <ul>
                                                     <ul>
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                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid">P-coumeric acid</a></li>
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                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid">P-coumaric acid</a></li>
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/resveratrol">Resveratrol</a></li>
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/resveratrol">Resveratrol</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/betacarotene">β-carotene</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/betacarotene">β-carotene</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/astraxantin">Astraxantin</a></li>
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                                                                <li><a
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<li><a href="https://2013.igem.org/Team:Uppsala/zeaxantin">Zeaxantin</a></li>
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href="https://2013.igem.org/Team:Uppsala/saffron">Saffron</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/astaxanthin">Astaxanthin</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/zeaxanthin">Zeaxanthin</a></li>
</ul></li>
</ul></li>
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</li>  
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<li><a href="https://2013.igem.org/Team:Uppsala/chromoproteins">Chromoprotein</a></li>
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                                                <li><a
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<li><a href="https://2013.igem.org/Team:Uppsala/safety">Safety experiments</a></li>
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href="https://2013.igem.org/Team:Uppsala/miraculin">Miraculin</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/result">Result</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/chromoproteins">Chromoproteins</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/safety-experiment">Safety experiment</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/results">Results</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/6/63/Uppsala2013_Modeling.png"></a>
<li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/6/63/Uppsala2013_Modeling.png"></a>
<ul>
<ul>
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<li><a href="https://2013.igem.org/Team:Uppsala/resveratrol-pathway">Resveratrol pathway </a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/P-Coumaric-acid-pathway">P-Coumaric acid</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/carotenoids-pathway">Carotenoids pathway </a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/modeling-tutorial">Modeling tutorial </a></li>
</ul></li>
</ul></li>
<li><a href="https://2013.igem.org/Team:Uppsala/parts" id="list_type2"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/e/eb/Uppsala2013_parts.png"></a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/parts" id="list_type2"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/e/eb/Uppsala2013_parts.png"></a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/carotenoid-group">Carotenoid group</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/carotenoid-group">Carotenoid group</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/chassi-group">Chassi group</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/chassi-group">Chassi group</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/advisors">Advisors</a></li>
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                                                <li><a href="https://2013.igem.org/Team:Uppsala/advisors">Advisors</a></li>
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</ul></li>
</ul></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/hp" id="list_type3"><img class="nav-text"      src="https://static.igem.org/mediawiki/2013/b/b8/Uppsala2013_HP.png"></a>
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<li><a href="https://2013.igem.org/Team:Uppsala/human-practice" id="list_type3"><img class="nav-text"      src="https://static.igem.org/mediawiki/2013/b/b8/Uppsala2013_HP.png"></a>
<ul>
<ul>
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<li><a href="https://2013.igem.org/Team:Uppsala/Yoghurtproducts">Yoghurt products</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/yoghurt">Yoghurt +</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/synbioday">SynBio Day</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/synbioday">SynBioDay</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/bioethics">Bioethics</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/biosafety-and-ethics">Biosafety and ethics</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/safety">Bioethics and Safety</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/public-opinion">Public opinion </a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/society">Society and Outreach</a></li>
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                                                <li><a href="https://2013.igem.org/Team:Uppsala/Outreach">High school & media </a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/highschool-outreach">High School Outreach</a></li>
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<li><a href="https://2013.igem.org/Team:Uppsala/bioart">BioArt</a></li>
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                                                <li><a href="https://2013.igem.org/Team:Uppsala/outreach">Media Outreach</a></li>
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</ul></li>
</ul></li>
<li><a href="https://2013.igem.org/Team:Uppsala/attribution" id="list_type4"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/5/5d/Uppsala2013_Attributions.png"></a></li>  
<li><a href="https://2013.igem.org/Team:Uppsala/attribution" id="list_type4"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/5/5d/Uppsala2013_Attributions.png"></a></li>  
<li><a href="https://2013.igem.org/Team:Uppsala/notebook" id="list_type3"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/3/36/Uppsala2013_Notebook.png"></a>
<li><a href="https://2013.igem.org/Team:Uppsala/notebook" id="list_type3"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/3/36/Uppsala2013_Notebook.png"></a>
                                     <ul>
                                     <ul>
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                                         <li><a href="https://2013.igem.org/Team:Uppsala/protocol">Protocol</a></li>
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                                         <li><a href="https://2013.igem.org/Team:Uppsala/safety-form">Safety form</a></li>
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                                         <li><a href="https://2013.igem.org/Team:Uppsala/lab-journal">Lab journal</a></li>
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                                         <li><a href="https://2013.igem.org/Team:Uppsala/protocols">Protocols</a></li>
                                     </ul></li>
                                     </ul></li>
</ul>
</ul>
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<div id="container">
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<img src="https://static.igem.org/mediawiki/2013/7/75/1379092_10152032735758413_677462372_n.jpg" style="width:900px;">
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<div id="project-description">
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<h1>
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Making the world healthier with a new probiotic platform
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</h1>
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                <div id="text-box">
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<p class="project-text"> Malnutrition is today a major global problem that affects people both in affluent and developing countries. Even if you get the right amount of calories, if these do not contain sufficient amounts of micronutrients, like vitamins and minerals, serious illness and even death can be the result.
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<div id="yoghurt-content">
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The goal of our project is to alleviate this problem by applying synthetic biology to <a href="https://2013.igem.org/Team:Uppsala/probiotics">probiotic bacteria</a>. These bacteria are already a part of normal human diet and our goal is to modify them so that they enrich the nutritional content of the food they grow in.<br><br>
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<h1 class="subheader">The next generation of synthetic probiotics</h1>
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With our project, we will make the lactobacillus genus the <a href="https://2013.igem.org/Team:Uppsala/chassi"> new probiotic platform </a> for <a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering"> metabolic engineering </a> of nutritional compounds. We will engineer probiotics to produce beta-carotene (provitamin), resveratrol (antioxidant), miraculin (sweetener) and saffron (taste).
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<p>Uppsala iGEM proudly presents a new revolutionizing way to produce food and healthy substances in probiotic bacteria. As a part of our human practice project, we have made a yoghurt as one of  our final goals. Of course, as a medium of carrying probiotic bacteria we are not only restricted to yoghurt. Our probiotics can be provided in a juice, pills and many different foods.<br><br>
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To exemplify what this combination of probiotics and metabolic engineering can accomplish we hope to use our modified bacteria to create nutritionally enriched yoghurt. We have also put great effort into addressing the <a href="https://2013.igem.org/Team:Uppsala/biosafety-and-ethics"> ethical and safety </a> issues that naturally follow when creating genetically modified food. As a part of this we organised a large event, <a href="https://2013.igem.org/Team:Uppsala/synbioday"> Uppsala SynBioDay </a>, with over 200 participants where these issues were discussed.
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                </div>
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The reasons we did this concept product is as following:</p>
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</p>
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</div>
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<div style="background-image:url('https://static.igem.org/mediawiki/2013/6/6c/Uppsala_cow_Upprop.png') o-repeat fixed center; background-size: 80% 80%; width: 100%; height: 200px;" ">
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<li class="yoghurt-text-list"><p>We felt that we early on in the project wanted to have a clear vision on what our final goal would be.</p></li>
 
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<li class="yoghurt-text-list"><p class="test">This concept product makes it easier for us to explain for the general public what we are doing. We have seen that media and people is more interested in our project, because they can envision exactly what we are trying to accomplish.</p></li>
 
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<li class="yoghurt-text-list"><p>By creating this yoghurt, we can use this as a tool to start a debate and discuss future applications of synthetic biology.</p></li>
 
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<br>
 
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<p>Our goal with this yoghurt is to make probiotic cultures produce all of our daily needs. When we use probiotics to produce nutritions, we do exactly what nature has done for us during all ages. With this, we can spare our nature, by going into the future with a totally new way to produce our food.<br>This is a yoghurt that contains everything in one drink. With this, people can forget about expensive pills and supplements.<br><br>
 
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Our nutritions are for example produced in the healthy probiotic culture lactobacillus reuteri, which in itself is very good for our wellbeing.<br><br>
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<img src="" style="width:80%;">
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      <div style="z-index: 1000;width: 300px; height: 50px; position: relative; top: 500x; left: 500px; border: 1px solid black;"><a href="" style="width: 100%; height: 100%;"></a></div>
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<div>
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<div id="grid-matrix">
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<div id="grid-a">
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In the end, all of the genes that produces the different nutritions will be incorporated in the chromosome of our probiotics. This will eliminate the need for antibiotic resistance markers on our final product, thus eliminating the risk of spreading antibiotic resistance in our nature and ingesting them in our stomach.</p>
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  <a href="https://2013.igem.org/Team:Uppsala/project#project-anchor"><img class="top" src="https://static.igem.org/mediawiki/2013/a/a3/Uppsala2013_Background_new.png"></a>
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</div>
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<div id="grid-b">
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                        <a href="https://2013.igem.org/Team:Uppsala/project#vision-anchor"><img class="top" src="https://static.igem.org/mediawiki/2013/1/13/Uppsala2013_Vision.png"></a>
</div>
</div>
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<div id="youtube">
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<div id="grid-c">
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<iframe width="390" height="219" src="http://www.youtube.com/embed/Bol07E6rt3c" frameborder="0" allowfullscreen></iframe>
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                        <a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering"><img class="top" src="https://static.igem.org/mediawiki/2013/4/4f/Uppsala2013_Metabolicengineering.png"></a>
</div>
</div>
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<div id="sidebar">
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<div id="grid-d">
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                        <a href="https://2013.igem.org/Team:Uppsala/chassi"><img class="top" src="https://static.igem.org/mediawiki/2013/1/19/Uppsala2013_Chassi.png"></a>
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</div>
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<h1 class="sidebar-header">The yoghurt will contain natural probiotic produced nutritions:</h1>
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<div id="grid-e">
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<li>P-coumaric acid</li>
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                        <a href="https://2013.igem.org/Team:Uppsala/human-practice"><img class="top" src="https://static.igem.org/mediawiki/2013/d/de/Uppsala2013_HumanPractice.png"></a>
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<li>Resveratrol</li>
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</div>
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<li>Beta carotene</li>
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<li>Miraculin</li>
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<li>Lycopene</li>
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<li>Astaxanthin</li>
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<li>Zeaxzanthin</li>
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<h1 class="sidebar-header">Also it will be made of:</h1>
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<div id="grid-f">
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<li>Milk</li>
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                        <a href="https://2013.igem.org/Team:Uppsala/results"><img class="top" src="https://static.igem.org/mediawiki/2013/a/a3/Uppsala2013_Achievements.png"></a>
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<li>Yoghurt culture</li>
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</div>
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<h1 class="sidebar-header">With synthetic biology we can also produces tastes ,like:</h1>
 
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<li>Saffron</li>
 
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<li>Vanillin</li>
 
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</div>
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</div>
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</div>
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<div id="sidebar">
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<div id="fb-feed">
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<iframe src="http://www.facebook.com/plugins/likebox.php?href=http://www.facebook.com/UppsalaIgem?fref=ts&width=292&colorscheme=light&show_fa
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ces=false&amp;border_color&amp;stream=true&amp;header=false&amp;height=475" scrolling="no" frameborder="0" style="border:none;
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overflow:hidden; width:310px; height:475px;" allowTransparency="true"></iframe>
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</div>
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<div id="twitter-feed">
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<a class="twitter-timeline" data-dnt="true" href="https://twitter.com/igemuppsala" data-widget-id="352909282024505345">Tweets av @igemuppsala</a>
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<script>!function(d,s,id){var js,fjs=d.getElementsByTagName(s)[0],p=/^http:/.test(d.location)?'http':'https';if(!d.getElementById(id)){js=d.createElement(s);js.id=id;js.src=p+"://platform.twitter.com/widgets.js";fjs.parentNode.insertBefore(js,fjs);}}(document,"script","twitter-wjs");</script>
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</div>
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</div> <!-- main_content ends -->
 
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<div id="bottom-pic">
 
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<img class="bottom-pic" src="https://static.igem.org/mediawiki/2013/a/aa/Bottom_picture.png">
 
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<div id="sponsor">
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<h1>Sponsors</h1>
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  <table class="sponsorTable">
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<tr class="colPush">
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<td class="centerThis"><img class="sponsors" src="https://static.igem.org/mediawiki/2013/a/a4/Uppsala_Clc.png"></td>
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<td><img class="sponsors" src="https://static.igem.org/mediawiki/2013/5/55/Uppsala_TekNat.png"></td><td><img class="sponsors" src="https://static.igem.org/mediawiki/2013/1/14/Uppsala_Analytik_Jena.png"></td></tr>
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Latest revision as of 15:12, 27 October 2013

Making the world healthier with a new probiotic platform

Malnutrition is today a major global problem that affects people both in affluent and developing countries. Even if you get the right amount of calories, if these do not contain sufficient amounts of micronutrients, like vitamins and minerals, serious illness and even death can be the result. The goal of our project is to alleviate this problem by applying synthetic biology to probiotic bacteria. These bacteria are already a part of normal human diet and our goal is to modify them so that they enrich the nutritional content of the food they grow in.

With our project, we will make the lactobacillus genus the new probiotic platform for metabolic engineering of nutritional compounds. We will engineer probiotics to produce beta-carotene (provitamin), resveratrol (antioxidant), miraculin (sweetener) and saffron (taste). To exemplify what this combination of probiotics and metabolic engineering can accomplish we hope to use our modified bacteria to create nutritionally enriched yoghurt. We have also put great effort into addressing the ethical and safety issues that naturally follow when creating genetically modified food. As a part of this we organised a large event, Uppsala SynBioDay , with over 200 participants where these issues were discussed.

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