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Team:Calgary/Sandbox/Notebook/Protocols/OvernightCultures
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<li>10mL culture tube. Use 16mm x 160mm or 16mm x 125mm</li> | <li>10mL culture tube. Use 16mm x 160mm or 16mm x 125mm</li> | ||
| - | <li> | + | <li>5mL LB</li> |
| - | <li> | + | <li>5μL 1000X antibiotics</li> |
<li>Single colonies on a plate (best not to start an overnight from a glycerol stock)</li> | <li>Single colonies on a plate (best not to start an overnight from a glycerol stock)</li> | ||
</ul> | </ul> | ||
Latest revision as of 01:25, 18 September 2013
Overnight Cultures
Reagents and Materials (per culture prepared):
- 10mL culture tube. Use 16mm x 160mm or 16mm x 125mm
- 5mL LB
- 5μL 1000X antibiotics
- Single colonies on a plate (best not to start an overnight from a glycerol stock)
Protocol
- Add 3mL sterile/autoclaved LB in a 15mL Falcon tube
- Pipet 3μL of 1000X antibiotic into the LB
- Select a single colony using a sterile toothpick or pipette tip
- Place toothpick or pipette tip in the culture tube and stir
- Remove toothpick, or in the case of a pipette tip, leave in the tube
- Place culture tube in incubator at 37 degrees Celsius overnight shaking vigorously (250 RPM)
