Latest revision as of 01:21, 5 October 2013

Notebook : August 23

Expect sizes :

We can't see anything. The digestion didn't work.

@@ Line 7: / Line 7: @@
 ==='''A - Aerobic/Anaerobic regulation system'''===
-===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3'''====
+===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in pSB3K3'''====
-(((((((((((((((===='''1 - Electrophoresis to check the digestion of Bba_J04450 by EcoRI/PstI'''====
+===='''1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI'''====
 {|
-| style="width:350px;border:1px solid black;" |]]
+| style="width:350px;border:1px solid black;" |[[File:Psgel2308.jpg|200px]]
 | style="width:350px;border:1px solid black;vertical-align:top;" |
 * Well 1 : 6µL DNA Ladder
-* Well 2 : 5µL of Bba_J04450 digested by EcoRI/PstI+1µl of 6X loading dye
+* Well 2 : 5µL of BBa_J04450 digested by EcoRI/PstI +1 µl of 6X loading dye
 * Gel : 1%
 |}
 Expect sizes :
-* PSB3K3 : 2750 bp
+* pSB3K3 : 2750 bp
 {|
 | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
-We can see anything. The digestion did work.
+We can't see anything. The digestion didn't work.
-|}))))))))))))))))))))))
+|}
+{| border="1" align="center"
+|[[Team:Paris Saclay/Notebook/August/22|<big>Previous day</big>]]
+|[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]]
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+|}
 {{Team:Paris_Saclay/incl_fin}}