"
Team:Calgary/Sandbox/Notebook/Protocols/VerificationDigest
From 2013.igem.org
(Difference between revisions)
(Created page with "{{Team:Calgary/Main}} <html> <section id="Content"> <h1>Verification Digest</h1> <h2>Reagents and Materials</h2> <ul> <li>Restriction enzymes</li> <li>Appropriate buffer</li> <l...") |
|||
| Line 9: | Line 9: | ||
<li>Appropriate buffer</li> | <li>Appropriate buffer</li> | ||
<li>Plasmid purified DNA</li> | <li>Plasmid purified DNA</li> | ||
| - | <li>1 | + | <li>1% <a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/AgaroseGelElectrophoresis">agarose gel</a></li> |
</ul> | </ul> | ||
<h2>Protocol</h2> | <h2>Protocol</h2> | ||
Latest revision as of 18:25, 18 September 2013
Verification Digest
Reagents and Materials
- Restriction enzymes
- Appropriate buffer
- Plasmid purified DNA
- 1% agarose gel
Protocol
- In a tube, add (per reaction):
- 4µL Plasmid purified DNA
- 1µL appropriate buffer
- 0.2µL enzyme 1
- 0.2µL enzyme 2
- 3.6µL ddH2O
- Incubate at 37 degrees Celsius for 1h
- Run 10µL in a 1% agarose gel at 100V
