Team:OUC-China

From 2013.igem.org

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<<html lang="en">
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<html lang="en">
<head>
<head>
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<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
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<meta name="viewport" content="width=device-width, initial-scale=1.0">
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<link href="http://oucast.com\igem\css\bootstrap.css" rel="stylesheet" media="screen">
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<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
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<link href="http://oucast.com\igem\css\bootstrap-responsive.css" rel="stylesheet" media="screen">
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<link href="http://netdna.bootstrapcdn.com/bootstrap/3.0.0/css/bootstrap.min.css" rel="stylesheet" media="screen">
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<link href="http://oucast.com\igem\css\docs.css" rel="stylesheet">
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<script src="http://code.jquery.com/jquery.min.js"></script>
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<style>
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<style type="text/css">
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/*the igem Global setting*/
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#p-logo{display:none;}
#p-logo{display:none;}
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#content
#content
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a:visited{
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padding:0;
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    display: inline-block;
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    margin: 25px 0 0;
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    padding: 9px 22px 7px;
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    font-weight: bold;
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 +
    border: 2px solid rgba(255,255,255,.4);
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 +
    .banner .btn:hover {
 +
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 +
    .banner .btn:active {
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h1 {color:#0099FF;
 
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-
}
 
-
li {font-weight:normal;
 
-
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-
}
 
 +
  .banner .btn, .banner .dot {
 +
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    filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
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 +
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 +
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a:not(.btn) {
+
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color: #00699E;
+
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transition: 300ms;
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a:hover:not(.btn),
+
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a:active:not(.btn) {
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-
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-
transform: translateY(-8px);
+
-
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+
-
hr.soften {
+
      cursor: pointer;
-
height: 1px;
+
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+
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+
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+
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+
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.nav a {
+
      -webkit-transition: background .5s, opacity .5s;
-
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+
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-
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+
      transition: background .5s, opacity .5s;
-
transition: 500ms;
+
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 +
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 +
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 +
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 +
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 +
 
 +
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 +
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 +
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 +
    right: 20px;
 +
    color: #fff;
 +
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 +
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 +
      display: inline;
 +
      padding-left: 10px;
 +
      cursor: pointer;
 +
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 +
 
 +
/*features*/
 +
.features {
 +
  overflow: hidden;
 +
  padding: 50px 0;
}
}
 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
.wrap {
 +
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 +
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 +
}
 +
/*footer*/
 +
footer{
 +
  margin-top: 100px;
 +
  background-color: #eceff1;
 +
  color: #bdc1c5;
 +
  font-size: 17px;
 +
  padding: 0;
 +
}
 +
.footer-banner{
 +
  background-color: #34495e;
 +
  color: #58F8D9;
 +
  margin-left: 42px;
 +
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 +
  padding: 0 30px 30px;
 +
}
 +
.footer-banner ul{
 +
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 +
}
 +
.footer-banner ul li{
 +
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 +
  line-height: 19px;
 +
  padding: 6px 0;
 +
}
 +
.footer-banner a{
 +
  color: #58F8D9;
 +
}
 +
.footer-banner a:hover{
 +
  text-decoration: none;
 +
}
 +
.footer-title{
 +
  margin: 0 0 22px;
 +
  padding-top: 21px;
 +
  color: #1abc9c;
 +
  font-weight: bold;
 +
}
 +
.footerleft a{
 +
  color: #bdc1c5;
 +
}
 +
.footerleft a:visited {
 +
  color: #bdc1c5;
 +
}
</style>
</style>
-
<meta name="viewport" content="width=device-width, initial-scale=1.0">
 
-
<meta name="description" content="">
 
-
<meta name="author" content="">
 
-
<title>Members</title>
 
</head>
</head>
-
 
<body>
<body>
-
<div class="navbar navbar-inverse navbar-fixed-top">
+
<div class="navbar navbar-inverse navbar-fixed-top" role="navigation">
-
      <div class="navbar-inner">
+
  <div class="navbar-header">
-
        <div class="container">
+
    <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-ex1-collapse">
-
<div class="row"><div class="span3"><a href="https://2013.igem.org/Main_Page"><img src="http://oucast.com/igem/img/igem1.jpg" alt="logo" style="width:70px;height:50px;margin-top:0px;padding:0 0 0 0;" class="brand png"></a></div>
+
      <span class="sr-only">The navigation</span>
-
          <button type="button" class="btn btn-navbar" data-toggle="collapse" data-target=".nav-collapse">
+
          <span class="icon-bar"></span>
-
            <span class="icon-bar"></span>
+
          <span class="icon-bar"></span>
-
            <span class="icon-bar"></span>
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          <span class="icon-bar"></span>
-
            <span class="icon-bar"></span>
+
        </button>
-
          </button>
+
    <a class="navbar-brand" href="https://igem.org/Team_Wikis?year=2013">
-
            <div class="nav-collapse collapse">
+
      <img src="https://static.igem.org/mediawiki/2013/7/7d/Ouc-igem1.png">
-
                  <ul class="nav pull-right">
+
    </a>
-
<li class="active"><a href="https://2013.igem.org/Team:OUC-China"><font size="4.5px">Home</font></a></li>
+
    <a class="navbar-brand" href="https://2013.igem.org/Team:OUC-China">
-
              <li class="dropdown">
+
      <p>OUC-China IGEM 2013</p>
-
                <a class="dropdown-toggle" id="drop4" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Project</font><b class="caret"></b>
+
    </a>
-
</a>
+
  </div>
-
                <ul id="menu1" class="dropdown-menu" role="menu" aria-labelledby="drop4">
+
    <div class="collapse navbar-collapse navbar-ex1-collapse">
-
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="#">Achievement & judge criteria</a>
+
      <ul class="nav navbar-nav pull-right">
-
                  <li class="dropdown-submenu">
+
      <li><a href="https://2013.igem.org/Team:OUC-China">Home</a></li>
-
                  <a tabindex="-1" href="#">Intercellular compartment</a>
+
            <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
-
  <ul class="dropdown-menu">
+
              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<b class="caret"></b></a>
-
                      <li><a tabindex="-1" href="#">Review</a></li>
+
              <ul class="dropdown-menu">
-
                      <li><a tabindex="-1" href="#">Design</a></li>
+
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Overview">Overview</a></li>
-
  <li><a tabindex="-1" href="#">Microfluidics</a></li>
+
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Achievement">Achievement & judge criteria</a></li>
-
  <li><a tabindex="-1" href="#">Results</a></li>
+
                    <li class="dropdown-submenu">
-
 
+
                      <a tabindex="-1" href="#">Intracellular compartment</a>
-
  </ul>
+
                <ul class="dropdown-menu">
-
  </li>
+
                        <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Review">Review</a></li>
 +
                        <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Design">Design</a></li>
 +
              <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Microfluidics</a></li>
 +
              <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Results">Results</a></li>
 +
                </ul>
 +
            </li>
                   <li class="dropdown-submenu">
                   <li class="dropdown-submenu">
                   <a tabindex="-1" href="#">RNA guardian</a>
                   <a tabindex="-1" href="#">RNA guardian</a>
-
  <ul class="dropdown-menu">
+
          <ul class="dropdown-menu">
-
                       <li><a tabindex="-1" href="#">Instruction</a></li>
+
                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Introduction">Introduction</a></li>
-
                       <li><a tabindex="-1" href="#">Design</a></li>
+
                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Design">Design</a></li>
-
  <li><a tabindex="-1" href="#">Result</a></li>
+
            <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Results">Result</a></li>
-
  <li><a tabindex="-1" href="#">Reference</a></li>
+
            <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Reference">Reference</a></li>
-
 
+
            </ul>
-
 
+
          </li>
-
  </ul>
+
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Part description">Part description</a></li>
-
  </li>
+
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Future work">Future work</a></li>
-
  <li role="presentation"><a role="menuitem" tabindex="-1" href="#">Part description</a></li>
+
-
                  <li role="presentation" class="divider"></li>
+
-
  <li role="presentation"><a role="menuitem" tabindex="-1" href="#">Future work</a></li>
+
                 </ul>
                 </ul>
-
              </li>
+
            </li>
-
              <li class="dropdown">
+
            <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
-
                <a class="dropdown-toggle" id="drop5" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Modeling</font><b class="caret"></b></a>
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            <h1>WE DID IT !</h1>
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            <p>Constructing an Artificial Organelle(Intracellular Compartment) in <i>E.coli</i></p>
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            <a class="btn" href="https://2013.igem.org/Team:OUC-China/Results">learn the story behind this picture</a>
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            <h1>Compression of Metabolic Pathways</h1>
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            <p>Constructing an artificial cluster to build the compartment by operating fewer genes. </p>
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            <a class="btn" href="https://2013.igem.org/Team:OUC-China/Design">learn more</a>
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            <h1>Microfluidic Chip Magnetism Detection</h1>
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            <h1>Factors Considered in RNA guardian</h1>
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  <h3>Zhong Xiaodong</h3>
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      <div class="span6"><p style="font-weight:normal;"><font size="3px">Hi! I’m Zhong Xiaodong. I major in biotechnology. I’m in charge of competition affairs and experiment. And you can contact me: zhongxiaodong@live.cn .I hope be friend with you.</font></p></div>
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      <h3><b>Features Of Our Project</b></h3><hr>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> Artificial Organelle(Intracellular Compartment)</b>
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          <p>We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.</p>
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          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Results">Learn more</a>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> RNA Guardian</b>
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          <p>We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.</p>
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          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Instruction">Learn more</a>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> Microfluidic</b>
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          <p>We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, <i>Magnetospirillum Magneticum</i>.</p>
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          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Learn more</a>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> Preserving <i>mam</i>AB genes</b>
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          <p>We preserved <i>Magnetospirillum Magneticum</i> AMB-1 <i>mam</i>AB genes in <i>E.coli</i>, prevented the genes lose when AMB-1 strain was cultured in high oxygen partial pressure environment.</p>
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      <div class="span6"><p style="font-weight:normal;"><font size="3px">My English name is Faye and I major in Life Sciences. My role in the iGEM team is lab assistance, competition affairs and advertising. I'm interested in Marine Microbiology, Biogeochemical cycles and lab management. I love exploring and I won't mind having a career that keeps me out at sea for most of the year. Besides biology, I enjoy classical literature, DIY, hiking and fantasy.</font></p></div>
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        <h3><b>Introduction</b></h3><hr>
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        <p>Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production.
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How could a membrane be constructed in a Prokaryote? The answer may lie in this species: <i>Magnetosprillum Magneticum</i>, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in <i><i>E.coli</i></i>, creating better conditions for efficient biological production.</p>
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        <p style="text-align:center"><a class="btn btn-large btn-info" href="https://2013.igem.org/Team:OUC-China/Overview">Learn more</a></p>
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Latest revision as of 03:38, 28 September 2013

    Features Of Our Project


  • Artificial Organelle(Intracellular Compartment)

    We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.

    Learn more
  • RNA Guardian

    We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.

    Learn more
  • Microfluidic

    We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, Magnetospirillum Magneticum.

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  • Preserving mamAB genes

    We preserved Magnetospirillum Magneticum AMB-1 mamAB genes in E.coli, prevented the genes lose when AMB-1 strain was cultured in high oxygen partial pressure environment.

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Introduction


Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production. How could a membrane be constructed in a Prokaryote? The answer may lie in this species: Magnetosprillum Magneticum, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in E.coli, creating better conditions for efficient biological production.

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