Team:Calgary/Notebook/Protocols/VerificationDigest

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<li>Appropriate buffer</li>
<li>Appropriate buffer</li>
<li>Plasmid purified DNA</li>
<li>Plasmid purified DNA</li>
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<li>1% <a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/AgaroseGelElectrophoresis">agarose gel</a></li>
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<li>1% <a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/AgaroseGelElectrophoresis" target="_blank">agarose gel</a></li>
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<h2>Protocol</h2>
<h2>Protocol</h2>
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<li>0.2µL enzyme 1</li>
<li>0.2µL enzyme 1</li>
<li>0.2µL enzyme 2</li>
<li>0.2µL enzyme 2</li>
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<li>3.6µL ddH2O</li>
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<li>3.6µL ddH<sub>2</sub>O (deionized water)</li>
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</ul>
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<li>Incubate at 37 degrees Celsius for 1h</li>
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<li>Incubate at 37°C for 1h</li>
<li>Run 10µL in a 1% agarose gel at 100V</li>
<li>Run 10µL in a 1% agarose gel at 100V</li>
</ol>
</ol>

Latest revision as of 00:22, 28 September 2013

Verification Digest

Reagents and Materials

  • Restriction enzymes
  • Appropriate buffer
  • Plasmid purified DNA
  • 1% agarose gel

Protocol

  1. In a tube, add (per reaction):
    • 4µL Plasmid purified DNA
    • 1µL appropriate buffer
    • 0.2µL enzyme 1
    • 0.2µL enzyme 2
    • 3.6µL ddH2O (deionized water)
  2. Incubate at 37°C for 1h
  3. Run 10µL in a 1% agarose gel at 100V

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