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Team:BYU Provo/Notebook/Cholera - Enzymes/September-October
From 2013.igem.org
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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> | ||
| - | : Cholera - Enzyme Notebook September'''</font> | + | : Cholera - Enzyme Notebook September-October'''</font> |
<br> | <br> | ||
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<font size = "4"> | <font size = "4"> | ||
| - | : <u> '''Cholera | + | : <u> '''Cholera Enzyme''' </u> </font> |
: [[Team:BYU Provo/Notebook/Cholera - Enzymes/March-April|March-April]] | : [[Team:BYU Provo/Notebook/Cholera - Enzymes/March-April|March-April]] | ||
| Line 26: | Line 26: | ||
: [[Team:BYU Provo/Notebook/Cholera - Enzymes/July-August|July-August]] | : [[Team:BYU Provo/Notebook/Cholera - Enzymes/July-August|July-August]] | ||
| - | : [[Team:BYU Provo/Notebook/Cholera - Enzymes/September-October|September]] | + | : [[Team:BYU Provo/Notebook/Cholera - Enzymes/September-October|September-October]] |
| + | |||
| + | : [[Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols|Protocols]] | ||
</font> | </font> | ||
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<br> | <br> | ||
| - | <font size="3" font face="Calibri"> | + | <font size="3" font face="Calibri"> We successfully prepared the DspB and AmyA plasmids to submit to the iGem registry and those to be used in our protein purification. We then were able to purify DspB and AmyA to use in our biofilm assay. </font> |
<br> | <br> | ||
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[[Team:BYU_Provo/Notebook/Cholera - Enzyme/September/Period1/Dailylog|Daily log]] | [[Team:BYU_Provo/Notebook/Cholera - Enzyme/September/Period1/Dailylog|Daily log]] | ||
| - | + | </font> | |
<br> | <br> | ||
| - | | style="width: 20%; background-color: transparent;"| [[File: | + | | style="width: 20%; background-color: transparent;"| [[File:Protein_GelAMYA927.jpg|250px|center|link=]] |
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| style="width: 18%; background-color: transparent;"| | | style="width: 18%; background-color: transparent;"| | ||
| - | | <font size="5" font face="Calibri"> '''September 15 - September | + | | <font size="5" font face="Calibri"> '''September 15 - September 30''' </font> |
<br> | <br> | ||
| - | <font size="3" font face="Calibri"> | + | <font size="3" font face="Calibri"> We focused on running our biofilm assay and getting data on the degradative enzymatic activity of the DspB and AmyA proteins we have purified.</font> |
<br> | <br> | ||
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<br> | <br> | ||
| + | | style="width: 20%; background-color: transparent;"| [[File:BYU2013-CholeraGroup2.jpg|250px|center]] | ||
| + | |- | ||
| + | | style="width: 18%; background-color: transparent;"| | ||
| + | | <font size="5" font face="Calibri"> '''October 1 - October 14''' </font> | ||
| - | + | <br> | |
| + | <font size="3" font face="Calibri"> We went to the North American iGem Jamboree and did well enough to make it to the World Championships in November. We got back to the lab extremely excited and motivated. We worked on finalizing our DspB plasmid to get it ready to over express and purify in ''E. coli''. </font> | ||
| + | |||
| + | <br> | ||
| + | |||
| + | <font color="#333399" size="4" font face="Calibri"> | ||
| + | |||
| + | [[Team:BYU_Provo/Notebook/Cholera - Enzyme/October/Period1/Dailylog|Daily log]] | ||
| + | |||
| + | </font> | ||
| + | |||
| + | <br> | ||
| + | |||
| + | | style="width: 20%; background-color: transparent;"| [[File:Protein_GelAMYA927.jpg|250px|center|link=]] | ||
| + | |||
| + | |- | ||
| + | |||
| + | | style="width: 18%; background-color: transparent;"| | ||
| + | |||
| + | | <font size="5" font face="Calibri"> '''October 15 - October 31''' </font> | ||
| + | |||
| + | <br> | ||
| + | |||
| + | <font size="3" font face="Calibri"> We finished purifying DspB and AmyA, ran Biofilm Assays to determine the effect of these enzymes on the biofilm of ''V. cholerae'', and planned future assays and experiments to continue this area of research. </font> | ||
| + | |||
| + | <br> | ||
| + | |||
| + | <font color="#333399" size="4" font face="Calibri"> | ||
| + | |||
| + | [[Team:BYU_Provo/Notebook/Cholera - Enzyme/October/Period2/Dailylog|Daily log]] | ||
| + | |||
| + | </font> | ||
| + | |||
| + | | style="width: 20%; background-color: transparent;"| [[File:Protein_GelAMYA927.jpg|250px|center|link=]] | ||
| + | |||
| + | |- | ||
| + | |||
| + | | style="width: 18%; background-color: transparent;"| | ||
|} | |} | ||
{{TeamBYUProvoFooter}} | {{TeamBYUProvoFooter}} | ||
Latest revision as of 23:57, 28 October 2013
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September 1 - September 14
We successfully prepared the DspB and AmyA plasmids to submit to the iGem registry and those to be used in our protein purification. We then were able to purify DspB and AmyA to use in our biofilm assay.
|  |
| September 15 - September 30
We focused on running our biofilm assay and getting data on the degradative enzymatic activity of the DspB and AmyA proteins we have purified.
|  | |
| October 1 - October 14
We went to the North American iGem Jamboree and did well enough to make it to the World Championships in November. We got back to the lab extremely excited and motivated. We worked on finalizing our DspB plasmid to get it ready to over express and purify in E. coli.
| | |
| October 15 - October 31
We finished purifying DspB and AmyA, ran Biofilm Assays to determine the effect of these enzymes on the biofilm of V. cholerae, and planned future assays and experiments to continue this area of research.
| | |
