Team:Calgary/Project/HumanPractices

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<h1>Human Practices</h1>
<h1>Human Practices</h1>
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<p>Our story this summer was a tumultuous adventure of consultation and rebuilding.  We had the initial idea of building a pathogenic <i>E. coli</i> detecting system in the early part of the year. Taking it from a mere idea to an actual system with a prototype took lots of communication and even more PCRs.</p>
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Within the context of our project, we chose to target a problem close to home- preventing contamination in beef products. Our goal is to detect enterohemorrhagic <i>E. coli</i> in beef products and minimize outbreaks. To deal with this pervasive problem in the beef industry, we consulted and worked with key "steakholders" at crucial points of the development process. These conversations shaped our entire project and are the foundation of our human practices. Our methodology toward human practices was to tailor our detection system to the end users of the system. We made the following achievements in our human practices:
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<p>Tracking back to the beginning of the year in our home province of Alberta we had just seen another enormous recall of beef that was contaminated with <i>E. coli</i> O157.  It had resulted in many deaths and even more cases of illnesses, and showed to us that the industry might have a challenge we could fit into.  We did a bit of research into the industry and got a feeling for how current methods worked on paper for detecting <i>E. coli</i>.  But to get a real sense of how the industry works we decided to talk with people who are actively involved in it every day.  The first person we spoke to was Bob Church, a rancher in Alberta.</p>
 
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<h2>Informed Design</h2>
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<li><p class="noIndent" style="clear: both;"><a href="https://2013.igem.org/Team:Calgary/Project/HumanPractices/InformedDesign">Informed design</span></a>: Input from four key industry stakeholders was used to redefine our project. Through these conversations, we assessed management of <i>E. coli</i> in the current industry and refocused our energy to build a novel tool for management of this pathogen prior to slaughter. We used input from these stakeholders to ensure that our system is easy to use, cost effective, and ultimately feasible for its application.</p>
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<p>Early on in our project we realized that we really needed to understand how the beef industry worked and who all the players were within it. We needed to understand the culture of the industry. To do so we reached out the first link we could think of, our families. Where did we get our meat from?  There is nothing more personal than the food we eat and understanding the last step in the long chain tells us a lot about the first step.  Speaking of that first step... </p>
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<li><p class="noIndent" style="clear: both;"><a href="https://2013.igem.org/Team:Calgary/Project/HumanPractices/Safety">Biological and user safety</span></a>: Our final prototype is comprised of <i>in vitro</i> protein components from recombinant bacteria. This design alleviates concerns over escape of synthetic organisms into the outside environment. We also thought about the sensitivity requirements for differentiating <i>E. coli</i> shedding in beef cattle, particularly with regard to false positives and false negatives. </p>
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<h2>Safety</h2>
 
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<p>While designing this project we also realized there was a very real concern from a safety perspective. The organisms we are targeting are listed as pathogens and can make people very sick. Thus we took a number of design considerations when building our technology.</p>
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Latest revision as of 00:36, 29 October 2013

Human Practices

Within the context of our project, we chose to target a problem close to home- preventing contamination in beef products. Our goal is to detect enterohemorrhagic E. coli in beef products and minimize outbreaks. To deal with this pervasive problem in the beef industry, we consulted and worked with key "steakholders" at crucial points of the development process. These conversations shaped our entire project and are the foundation of our human practices. Our methodology toward human practices was to tailor our detection system to the end users of the system. We made the following achievements in our human practices:

  • Informed design: Input from four key industry stakeholders was used to redefine our project. Through these conversations, we assessed management of E. coli in the current industry and refocused our energy to build a novel tool for management of this pathogen prior to slaughter. We used input from these stakeholders to ensure that our system is easy to use, cost effective, and ultimately feasible for its application.

  • Biological and user safety: Our final prototype is comprised of in vitro protein components from recombinant bacteria. This design alleviates concerns over escape of synthetic organisms into the outside environment. We also thought about the sensitivity requirements for differentiating E. coli shedding in beef cattle, particularly with regard to false positives and false negatives.

Informed Design

Safety

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