Team:OUC-China/Review

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<title>Review</title>
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<title>Overview</title>
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  <li class="active"><a href="https://2013.igem.org/Team:OUC-China"><font size="4.5px">Home</font></a></li>
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      <p>OUC-China IGEM 2013</p>
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                <a class="dropdown-toggle" id="drop4" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Project</font><b class="caret"></b>
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<li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Overview">Overview</a>
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      <ul class="nav navbar-nav pull-right">
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                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Achievement">Achievement & judge criteria</a>
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      <li><a href="https://2013.igem.org/Team:OUC-China">Home</a></li>
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                  <a tabindex="-1" href="#">Intercellular compartment</a>
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              <ul class="dropdown-menu">
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                      <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Review">Review</a></li>
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          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Overview">Overview</a></li>
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                      <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Design">Design</a></li>
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                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Achievement">Achievement & judge criteria</a></li>
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  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Microfluidics</a></li>
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                    <li class="dropdown-submenu">
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  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Results">Results</a></li>
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                      <a tabindex="-1" href="#">Intracellular compartment</a>
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                <ul class="dropdown-menu">
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  </ul>
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                        <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Review">Review</a></li>
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  </li>
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                        <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Design">Design</a></li>
 +
              <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Microfluidics</a></li>
 +
              <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Results">Results</a></li>
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                </ul>
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            </li>
                   <li class="dropdown-submenu">
                   <li class="dropdown-submenu">
                   <a tabindex="-1" href="#">RNA guardian</a>
                   <a tabindex="-1" href="#">RNA guardian</a>
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                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Instruction">Instruction</a></li>
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                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Introduction">Introduction</a></li>
                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Design">Design</a></li>
                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Design">Design</a></li>
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  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Results">Result</a></li>
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            <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Results">Result</a></li>
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  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Reference">Reference</a></li>
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            <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Reference">Reference</a></li>
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            </ul>
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          </li>
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          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Part description">Part description</a></li>
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  </li>
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          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Future work">Future work</a></li>
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  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Part description">Part description</a></li>
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                  <li role="presentation" class="divider"></li>
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  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Future work">Future work</a></li>
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                 </ul>
                 </ul>
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              </li>
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            </li>
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                <a class="dropdown-toggle" id="drop5" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Modeling</font><b class="caret"></b></a>
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              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<b class="caret"></b></a>
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                 <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="drop5">
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                 <ul class="dropdown-menu">
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Magnetic Analysis">Magnetic Analysis</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Magnetic Analysis">Magnetic Analysis</a></li>
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                  <li role="presentation" class="divider"></li>
 
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Model in RNA guardian">Model in RNA guardian</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Model in RNA guardian">Model in RNA guardian</a></li>
                 </ul>
                 </ul>
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            </li>
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              <li class="dropdown">
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            <li><a  href="https://2013.igem.org/Team:OUC-China/Human Practice">Human Practice</a></li>
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                <a  href="https://2013.igem.org/Team:OUC-China/Human Practice"><font size="4.5px">Human Practice</font></a>
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              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<b class="caret"></b></a>
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                <a class="dropdown-toggle" id="drop5" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Team</font><b class="caret"></b></a>
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              <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="drop5">
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                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Members">Members</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Members">Members</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Instructors">Instructors</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Instructors">Instructors</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Advisor">Advisor</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Advisor">Advisor</a></li>
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  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab">Lab</a></li>
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          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab">Lab</a></li>
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  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Acknowledgement">Acknowledgement</a></li>
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          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Acknowledgement">Acknowledgement</a></li>
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      <li class="dropdown"><a  href="https://2013.igem.org/Team:OUC-China/Safety">Safety</a></li>
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                <a  href="https://2013.igem.org/Team:OUC-China/Safety"><font size="4.5px">Safety</font></a>
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    <p>The intracellular compartment is so useful in protein embellishment that we are willing to construct such a compartment in E.coli cells. But what should we do to get it?<br/><br />An article from Nature 25 April 2013 prompts us that the magnetosome compartments in Magnetospirillum Magneticum can provide a reaction condition isolated from the cytoplasm to achieve intracellular biomineralization reaction. [1,2,3] This inspires us to construct the magnetosome compartments in prokaryote cells in order to improve the reaction rate, display protein molecules on membrane, and offer some research thought in many other aspects of the basic research of cell biology.<br/><br />Above all, we plan to construct an artificial gene cluster with several different genes: mamK, mamI, mamL, mamB and mamQ[4~12], to construct the compartment.<br /><br /><img src="https://static.igem.org/mediawiki/2013/8/82/Ouc-review.jpg" style="
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    width: 800px;"/><br />Fig1:our aim is to reconstruct Magnetosome membrane in <i>E.coli</i><br /><br />After gene cluster constructing, we propose to examine our result by (1)Using TEM to show the structure of the compartment; (2) Using MamC::GFP fusion protein to prove the possibility of anchoring protein onto membrane exactly and (3) Using a microfluidic chip which could characterize the magnetism of the cell.</p>
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      <div class="span9"><p style="font-weight:normal;"><font size="2px">γ€€    &nbsp;&nbsp;The intracellular compartment is so useful in protein embellishment that we are willing to construct such a compartment in E.coli cells. But what should we do to get it?<br/>
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γ€€γ€€An article from Nature 25 April 2013 prompts us that the magnetosome compartments in Magnetospirillum Magneticum can provide a reaction condition isolated from the cytoplasm to achieve intracellular biomineralization reaction. [1,2,3] This inspires us to construct the magnetosome compartments in prokaryote cells in order to improve the reaction rate, display protein molecules on membrane, and offer some research thought in many other aspects of the basic research of cell biology.<br/>
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  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Above all, we plan to construct an artificial gene cluster with several different genes: mamK, mamI, mamL, mamB and mamQ[4~12], to construct the compartment.<br />&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;After gene cluster constructing, we propose to examine our result by (1)Using TEM to show the structure of the compartment; (2) Using MamC::GFP fusion protein to prove the possibility of anchoring protein onto membrane exactly and (3) Using a microfluidic chip which could characterize the magnetism of the cell.</font></p>
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Latest revision as of 03:51, 28 September 2013

Overview

Review

The intracellular compartment is so useful in protein embellishment that we are willing to construct such a compartment in E.coli cells. But what should we do to get it?

An article from Nature 25 April 2013 prompts us that the magnetosome compartments in Magnetospirillum Magneticum can provide a reaction condition isolated from the cytoplasm to achieve intracellular biomineralization reaction. [1,2,3] This inspires us to construct the magnetosome compartments in prokaryote cells in order to improve the reaction rate, display protein molecules on membrane, and offer some research thought in many other aspects of the basic research of cell biology.

Above all, we plan to construct an artificial gene cluster with several different genes: mamK, mamI, mamL, mamB and mamQ[4~12], to construct the compartment.


Fig1:our aim is to reconstruct Magnetosome membrane in E.coli

After gene cluster constructing, we propose to examine our result by (1)Using TEM to show the structure of the compartment; (2) Using MamC::GFP fusion protein to prove the possibility of anchoring protein onto membrane exactly and (3) Using a microfluidic chip which could characterize the magnetism of the cell.

Retrieved from "http://2013.igem.org/Team:OUC-China/Review"