"
Team:INSA Toulouse/contenu/lab practice/notebook/calendar/general inducer
From 2013.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 166: | Line 166: | ||
BBa_I13521: Ptet rbs RFP term.</li> | BBa_I13521: Ptet rbs RFP term.</li> | ||
</ul> | </ul> | ||
| - | </li | + | </li> |
</div> | </div> | ||
</div> | </div> | ||
| Line 213: | Line 213: | ||
<img src="https://static.igem.org/mediawiki/2013/5/59/General_inducer5_-_h140px.png" class="imgcontent" /><br> | <img src="https://static.igem.org/mediawiki/2013/5/59/General_inducer5_-_h140px.png" class="imgcontent" /><br> | ||
This one seems to work but we are waiting for the sequencing to confirm the construct.<br><br></li> | This one seems to work but we are waiting for the sequencing to confirm the construct.<br><br></li> | ||
| - | + | </div> | |
| - | + | ||
| - | + | ||
</div> | </div> | ||
| + | <div class="clear"></div> | ||
<div class="clear"></div> | <div class="clear"></div> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
<p class="texte"> | <p class="texte"> | ||
<img src="https://static.igem.org/mediawiki/2013/3/3a/Back_arrow_-_14px.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/calendar/wet_lab">Back to Wet Lab</a></p> | <img src="https://static.igem.org/mediawiki/2013/3/3a/Back_arrow_-_14px.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/calendar/wet_lab">Back to Wet Lab</a></p> | ||
Latest revision as of 17:35, 4 October 2013
Calendar
General Inducer Characterization
June 2013
- Week 3 (24-30 June)
Amplification of new biobricks for cloning:
BBa_J23119: Strong promoter,
BBa_P0440: rbs tetR term,
BBa_I13521: Ptet rbs RFP term.
July 2013
- Week 4 (1-7 July)
Assembly of a strong promoter with tetR. tetR protein is necessary to inhibit the Ptet promoter.
- Week 5 (8-14 July)
Assembly of Strong promoter-tetR with Ptet-RFP. The RFP should be inhibited by tetR and expressed in presence of atc.
The construction is then sent for sequencing.
- Week 6 (15-21 July)
The sequencing shows that the construction include a mutated promoter instead of J23119. So we assembled tetR with Ptet-RFP.
- Week 7 (22-28 July)
We assembled a Strong promoter with tetR-Ptet-RFP to finalize the construction.
August 2013
- August 2013
- Week 8 (29-4 August)
The tries didn’t work, so we did it again one more week the assembly of Strong Promoter with tetR-Ptet-RFP. - Week 9 (5-11 August)
Because none of last clonages worked, we tried with several promoters in order to increase our chances of success.
- Week 10 (12-18 August)
None of the passed assemblies worked, so we tried with a weak promoter.
This one seems to work but we are waiting for the sequencing to confirm the construct.
- Week 8 (29-4 August)
