Team:OUC-China

From 2013.igem.org

(Difference between revisions)
(Undo revision 204690 by PengYong (talk))
 
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</head>
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<body >
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<body>
<div class="navbar navbar-inverse navbar-fixed-top" role="navigation">
<div class="navbar navbar-inverse navbar-fixed-top" role="navigation">
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  <div class="navbar-header">
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    <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-ex1-collapse">
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<span class="sr-only">The navigation</span>
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      <span class="sr-only">The navigation</span>
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      <span class="icon-bar"></span>
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          <span class="icon-bar"></span>
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      </button>
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     <a class="navbar-brand" href="https://igem.org/Team_Wikis?year=2013">
     <a class="navbar-brand" href="https://igem.org/Team_Wikis?year=2013">
       <img src="https://static.igem.org/mediawiki/2013/7/7d/Ouc-igem1.png">
       <img src="https://static.igem.org/mediawiki/2013/7/7d/Ouc-igem1.png">
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   </div>
   </div>
     <div class="collapse navbar-collapse navbar-ex1-collapse">
     <div class="collapse navbar-collapse navbar-ex1-collapse">
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    <ul class="nav navbar-nav pull-right">
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      <ul class="nav navbar-nav pull-right">
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<li><a href="https://2013.igem.org/Team:OUC-China">Home</a></li>
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      <li><a href="https://2013.igem.org/Team:OUC-China">Home</a></li>
             <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
             <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
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            <a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<b class="caret"></b></a>
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              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<b class="caret"></b></a>
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            <ul class="dropdown-menu">
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              <ul class="dropdown-menu">
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<li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Overview">Overview</a></li>
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          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Overview">Overview</a></li>
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                <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Achievement">Achievement & judge criteria</a></li>
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                  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Achievement">Achievement & judge criteria</a></li>
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                  <li class="dropdown-submenu">
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                    <li class="dropdown-submenu">
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                  <a tabindex="-1" href="#">Intercellular compartment</a>
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                      <a tabindex="-1" href="#">Intracellular compartment</a>
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  <ul class="dropdown-menu">
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                <ul class="dropdown-menu">
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                      <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Review">Review</a></li>
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                        <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Review">Review</a></li>
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                      <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Design">Design</a></li>
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                        <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Design">Design</a></li>
-
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Microfluidics</a></li>
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              <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Microfluidics</a></li>
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  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Results">Results</a></li>
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              <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Results">Results</a></li>
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  </ul>
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                </ul>
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  </li>
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            </li>
                   <li class="dropdown-submenu">
                   <li class="dropdown-submenu">
                   <a tabindex="-1" href="#">RNA guardian</a>
                   <a tabindex="-1" href="#">RNA guardian</a>
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  <ul class="dropdown-menu">
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          <ul class="dropdown-menu">
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                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Instruction">Instruction</a></li>
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                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Introduction">Introduction</a></li>
                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Design">Design</a></li>
                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Design">Design</a></li>
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  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Results">Result</a></li>
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            <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Results">Result</a></li>
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  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Reference">Reference</a></li>
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            <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Reference">Reference</a></li>
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  </ul>
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            </ul>
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  </li>
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          </li>
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  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Part description">Part description</a></li>
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          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Part description">Part description</a></li>
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  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Future work">Future work</a></li>
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          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Future work">Future work</a></li>
                 </ul>
                 </ul>
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          </li>
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            </li>
             <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
             <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
-
            <a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<b class="caret"></b></a>
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              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<b class="caret"></b></a>
                 <ul class="dropdown-menu">
                 <ul class="dropdown-menu">
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Magnetic Analysis">Magnetic Analysis</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Magnetic Analysis">Magnetic Analysis</a></li>
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             </li>
             </li>
             <li><a  href="https://2013.igem.org/Team:OUC-China/Human Practice">Human Practice</a></li>
             <li><a  href="https://2013.igem.org/Team:OUC-China/Human Practice">Human Practice</a></li>
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<li class="dropdown">
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      <li class="dropdown">
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            <a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<b class="caret"></b></a>
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              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<b class="caret"></b></a>
-
            <ul class="dropdown-menu" role="menu" aria-labelledby="dropdownMenu">
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              <ul class="dropdown-menu" role="menu" aria-labelledby="dropdownMenu">
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Members">Members</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Members">Members</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Instructors">Instructors</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Instructors">Instructors</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Advisor">Advisor</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Advisor">Advisor</a></li>
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  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab">Lab</a></li>
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          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab">Lab</a></li>
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  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Acknowledgement">Acknowledgement</a></li>
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          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Acknowledgement">Acknowledgement</a></li>
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</ul>
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        </ul>
             </li>
             </li>
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<li class="dropdown"><a  href="https://2013.igem.org/Team:OUC-China/Safety">Safety</a></li>
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      <li class="dropdown"><a  href="https://2013.igem.org/Team:OUC-China/Safety">Safety</a></li>
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<li class="dropdown">
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      <li class="dropdown">
                 <a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook<b class="caret"></b></a>
                 <a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook<b class="caret"></b></a>
                 <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="dropdownMenu">
                 <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="dropdownMenu">
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                 </ul>
                 </ul>
             </li>
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    </ul>
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</div>
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  </div>
</div>     
</div>     
<!--the slidershow begin-->
<!--the slidershow begin-->
<div class="banner">
<div class="banner">
       <ul>
       <ul>
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         <li style="background-image: url('https://static.igem.org/mediawiki/2013/2/24/Slider-wenjun-result.jpg');">
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         <li style="background-image: url('https://static.igem.org/mediawiki/igem.org/9/9e/Tital.jpg');">
           <div class="inner">
           <div class="inner">
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             <h1>WE DID IT!</h1>
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             <h1>WE DID IT !</h1>
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             <p>Constructing an Artificial Organelle in <i>E.coli</i></p>
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             <p>Constructing an Artificial Organelle（Intracellular Compartment） in <i>E.coli</i></p>
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             <a class="btn" href="https://2013.igem.org/Team:OUC-China/Results">learn more</a>
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             <a class="btn" href="https://2013.igem.org/Team:OUC-China/Results">learn the story behind this picture</a>
           </div>
           </div>
         </li>
         </li>
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         <li style="background-image: url('http://www.bootcss.com/p/unslider/img/wood.jpg');">
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         <li style="background-image: url('https://static.igem.org/mediawiki/2013/7/7d/Cluster.png');">
           <div class="inner">
           <div class="inner">
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             <h1>Come on! OUC IGEM 2013</h1>
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             <h1>Compression of Metabolic Pathways</h1>
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             <p>不要着急！我们可以弄完的啦～</p>
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             <p>Constructing an artificial cluster to build the compartment by operating fewer genes. </p>
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             <a class="btn" href="https://2013.igem.org/Team:OUC-China/Design">learn more</a>
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             <a class="btn" href="http://jing.fm/tracks/225c3f.html">来，听首歌轻松下！</a>
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           </div>
           </div>
         </li>
         </li>
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        <li style="background-color:#1abc9c;">
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  <li style="background-image: url('https://static.igem.org/mediawiki/2013/b/bc/Slider-xiaodong.jpg');">
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           <div class="inner">
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           <div class="inner" style="float:left;padding-left:30px;">
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             <h1>Come on! OUC IGEM 2013</h1>
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             <h1>Microfluidic Chip Magnetism Detection</h1>
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             <p>不要着急！我们可以弄完的啦～</p>
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             <p><br></p>
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             <a class="btn" href="https://2013.igem.org/Team:OUC-China/Microfluidics" style="color:#1abc9c;">learn more</a>
-
             <a class="btn" href="http://jing.fm/tracks/225c3f.html">来，听首歌轻松下！</a>
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           </div>
           </div>
         </li>
         </li>
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         <li style="background-image: url('https://static.igem.org/mediawiki/2013/7/75/Ouc-slide4.jpg');">
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         <li style="background-color:#1abc9c;">
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           <div class="inner">
           <div class="inner">
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             <h1>Come on! OUC IGEM 2013</h1>
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             <h1>Factors Considered in RNA guardian</h1>
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             <p>不要着急！我们可以弄完的啦～</p>
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             <p><br /></p>
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             <a class="btn" href="https://2013.igem.org/Team:OUC-China/RNA_guardian/Design">Learn more</a>
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             <a class="btn" href="http://jing.fm/tracks/225c3f.html">来，听首歌轻松下！</a>
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           </div>
           </div>
         </li>
         </li>
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        <h1>Abstract</h1>
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         <p class="lead">Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production.
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      <h3><b>Features Of Our Project</b></h3><hr>
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How could a membrane be constructed in a Prokaryote? The answer may lie in this species: Magnetosprillum Magneticum, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in E.coli, creating better conditions for efficient biological production.</p>
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        <a class="btn btn-large btn-success" href="#">Learn more</a>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> Artificial Organelle(Intracellular Compartment)</b>
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          <p>We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.</p>
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          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Results">Learn more</a>
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        <h1>Specialist of our project</h1>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> RNA Guardian</b>
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        <p class="lead">1.We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.<br/>2.We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.<br/>3.We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, Magnetospirillum Magneticum.<br/>4.We preserved Magnetospirillum Magneticum AMB-1 mamAB genes in E.coli, prevented the genes lose when AMB-1 strain was cultured in High oxygen partial pressure environment.
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          <p>We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.</p>
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          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Instruction">Learn more</a>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> Microfluidic</b>
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          <p>We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, <i>Magnetospirillum Magneticum</i>.</p>
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          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Learn more</a>
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        <h4><img src="https://static.igem.org/mediawiki/2013/6/6f/Ouc-title.png" alt="OUC-China"></h4>
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         <p class="lead" ><font color="#0099FF">Reconstructing the Magnetosome Membrane in E. coli</font></p>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> Preserving <i>mam</i>AB genes</b>
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          <p>We preserved <i>Magnetospirillum Magneticum</i> AMB-1 <i>mam</i>AB genes in <i>E.coli</i>, prevented the genes lose when AMB-1 strain was cultured in high oxygen partial pressure environment.</p>
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          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Design">Learn more</a>
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        <h3><b>Introduction</b></h3><hr>
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        <p>Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production.
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How could a membrane be constructed in a Prokaryote? The answer may lie in this species: <i>Magnetosprillum Magneticum</i>, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in <i><i>E.coli</i></i>, creating better conditions for efficient biological production.</p>
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         <p style="text-align:center"><a class="btn btn-large btn-info" href="https://2013.igem.org/Team:OUC-China/Overview">Learn more</a></p>
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  <h3 class="footer-title" style="color:#bdc1c5;">About</h3>
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Latest revision as of 03:38, 28 September 2013

    Features Of Our Project

  • Artificial Organelle(Intracellular Compartment)

    We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.

    Learn more
  • RNA Guardian

    We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.

    Learn more
  • Microfluidic

    We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, Magnetospirillum Magneticum.

    Learn more
  • Preserving mamAB genes

    We preserved Magnetospirillum Magneticum AMB-1 mamAB genes in E.coli, prevented the genes lose when AMB-1 strain was cultured in high oxygen partial pressure environment.

    Learn more

Introduction

Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production. How could a membrane be constructed in a Prokaryote? The answer may lie in this species: Magnetosprillum Magneticum, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in E.coli, creating better conditions for efficient biological production.

Learn more

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