"
Team:CU-Boulder/RecycleValidation
From 2013.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 11: | Line 11: | ||
<center> | <center> | ||
<p><b> | <p><b> | ||
| - | Columns were used to purify RFP expressing plasmid. Columns were recycled as per the recycling protocol (left) or not (right) and then used to purify AmilCP expressing plasmid. The elutant was transformed and plated. Recycled columns resulted in 100% AmilCP (blue) producing colonies. Non-recycled columns produced ~ 50% AmilCP (blue) producing colonies and ~50% RFP producing colonies from the previous | + | Columns were used to purify RFP (red) expressing plasmid. Columns were recycled as per the recycling protocol (left) or not (right) and then used to purify AmilCP (blue) expressing plasmid. The elutant was transformed and plated. Recycled columns resulted in 100% AmilCP (blue) producing colonies. Non-recycled columns produced ~ 50% AmilCP (blue) producing colonies and ~50% RFP producing colonies resulted from contaminating plasmid from the previous purification. Recycling works. |
</p> | </p> | ||
</center> | </center> | ||
Latest revision as of 19:07, 27 September 2013
Column Recycling Protocol Validation
Columns were used to purify RFP (red) expressing plasmid. Columns were recycled as per the recycling protocol (left) or not (right) and then used to purify AmilCP (blue) expressing plasmid. The elutant was transformed and plated. Recycled columns resulted in 100% AmilCP (blue) producing colonies. Non-recycled columns produced ~ 50% AmilCP (blue) producing colonies and ~50% RFP producing colonies resulted from contaminating plasmid from the previous purification. Recycling works.
