Spatial Modelling

Spatial modelling

In order to get a sense for how to optimally assemble our reporter and detector molecules for integration with the prototype, we used Autodesk Maya to build and analyze potential capture protein fusions in silico. In contemplating the prototype design of capture TALE detectors in the nitrocellulose lateral flow strip prototype, the wetlab team conceived the following protein fusion designs:

We were concerned about how the interaction of TALE detectors with the nitrocellulose substrate might reduce their sensitivity. The wetlab asked the modelling team who proposed simulating the capture proteins and nitrocellulose in Maya to answer this question. In order to determine potential for steric hinderance issues with DNA binding, we needed to know how DNA and TALEs bind together. The steps in this interaction have not been previously documented, so we animated a putative interaction in Maya. See Figure 2 below.

The interaction between the TALE and DNA appears to require intricate contortions of the TALE and space around the periphery of the protein. The next step was to use Maya to show how the interaction of DNA with the TALE is influenced by association of the TALE with a surface such as nitrocellulose. See Figure 3 below.

In Figure 3A, the TALE is associated directly with a surface representing nitrocellulose, whereas in 3B, it is separated by linker sequence separating the TALE from the surface. Given the geometric constraints of the TALE-DNA interaction shown in Figure 2, the modelling team concluded that capture TALEs in the system should be bound to a protein to separate detector from nitrocellulose. From this data, the wetlab will be focussing prototype development where capture TALEs are fused to ferritin to separate them from nitrocellulose (see Figures 1B and 3B).

Animation of complete system

From the basic protein components imported into Maya from spatial modelling, we repurposed these elements to create a compelling animation to explain our completed system to tell our story. We wanted to show the complete system in a cohesive video to efficiently explain how the molecular interactions of the FerriTALE output a signal on the final prototype. Please see the video below:

As seen in the video above our goal is to develop a strip based assay that can alert us upon detection of pathogenic DNA sequence, namely stx2. We will treat our sample collected from the supershedders with a TALE-Ferritin complex (a FerriTALE!). Then this DNA-TALE-Ferritin complex would be flown over our strip which contains the second TALE that recognizes yet another 18-20 bp region on the stx2 gene. This second TALE will immobilize our DNA-TALE-ferritin complex on the strip. Following immobilization we will add substrate to our strip. Only in the presence of both the EHEC sequences we get a colour change on the strip. If there is only one of the sequences present, the colour change does not occur either due to lack of immobilization or due to lack of presence of a reporter.

3D Printing:

Through our collaboration with Cesar Rodriguez, a Senior Research Scientist in the Bio/Nano/Programmable Matter group at Autodesk, we have been able to expand our spatial modelling beyond the computer screen. He and his team have offered us access to the 3D printing services at Autodesk to make a tangible model of our molecules based off our Maya simulations. This has allowed our team to get a feel for the shape of our proteins, aiding the design of our prototype, linkers, and the system as a whole.