Team:Paris Saclay/Notebook/August/7

From 2013.igem.org

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==='''A - Aerobic/Anaerobic regulation system'''===
==='''A - Aerobic/Anaerobic regulation system'''===
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===='''Objective : obtaining Bba_K1155007'''====
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===='''Objective : obtaining BBa_K1155003'''====
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===='''1 - Tranformation Bba_K115507 in DH5α====
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===='''1 - Sequensis analysis of BBa_K1155003'''====
 +
 
 +
Abdou
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 +
{|
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| style="border:1px solid black;padding:5px;background-color:#DE;" |
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The sequence analysis was good. We call our biobrick BBa_K1155003.
 +
|}
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 +
===='''Objective : obtaining BBa_K1155007'''====
 +
 
 +
===='''1 - Ligation of RBS-LacZ-Term in pSB1C3 ====
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 +
Anaïs, Nadia
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 +
Used quantities :
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* RBS-AmilCP : 2µL
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* Term in pSB1C3 : 2.5µL
 +
* Buffer : 1µL
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* Ligase : 1µL
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* H2O : 3.5µL
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 +
===='''2 - Tranformation BBa_K115507 in DH5αstrain====
XiaoJing
XiaoJing
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Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
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===='''Objective : characterize Bba_K1155000, Bba_K1155004, Bba_K1155005, Bba_K1155006'''====
+
===='''Objective : characterize BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006'''====
===='''1 - Tranduction of Km in MG1655Z1 ====
===='''1 - Tranduction of Km in MG1655Z1 ====
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Nadia, XioaJing
+
Nadia, XiaoJing
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Protocol : [[Team:Paris_Saclay/Protocols/transduction|Transduction]]
+
Protocol : [[Team:Paris_Saclay/transduction|Transduction]]
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Our Mutant bacteria was called BW : Δfnr::Km.
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Wild type strain is MG1655Z1 and our mutant strain is BW1328: Δfnr::Km.
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Our wild type bacteria was called MG1655Z1.
+
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We did the first step of the protocol : bacteriophage stock which packed Km gene.
+
We did the first step of the protocol : bacteriophage stock grown on BW1328.
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Latest revision as of 21:19, 4 October 2013

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Contents

Notebook : August 7

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155003

1 - Sequensis analysis of BBa_K1155003

Abdou

The sequence analysis was good. We call our biobrick BBa_K1155003.

Objective : obtaining BBa_K1155007

1 - Ligation of RBS-LacZ-Term in pSB1C3

Anaïs, Nadia

Used quantities :

  • RBS-AmilCP : 2µL
  • Term in pSB1C3 : 2.5µL
  • Buffer : 1µL
  • Ligase : 1µL
  • H2O : 3.5µL

2 - Tranformation BBa_K115507 in DH5αstrain

XiaoJing

Protocol : Bacterial transformation

Objective : characterize BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006

1 - Tranduction of Km in MG1655Z1

Nadia, XiaoJing

Protocol : Transduction

Wild type strain is MG1655Z1 and our mutant strain is BW1328: Δfnr::Km.

We did the first step of the protocol : bacteriophage stock grown on BW1328.


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