Team:TU-Delft/ProtocolList

From 2013.igem.org

(Difference between revisions)

Latest revision as of 20:38, 4 October 2013

Protocols

Our project deals with E.coli cells which sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and starts producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Different protocols used during the project are described below.

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-<div style="margin-left:30px;margin-right:30px; width:900px;float:left;">
+<div style="margin-left:30px;margin-right:30px;margin-top:-20px; width:900px;float:left;">
 <h2 align="center">Protocols</h2>
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 <ul style="list-style-type: circle">
-   <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_1#Protocol_1" style="text-decoration: none""><font color="#0080FF" size="3">Transforming Parts from Distribution kit</font></a> </li>
+   <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_1#protocol_1" style="text-decoration: none""><font color="#0080FF" size="3">Transforming Parts from Distribution kit</font></a> </li>
-  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol2#Protocol_2" style="text-decoration: none""><font color="#0080FF" size="3">Growing the Single Colonies from the Agar Plates</font></a> </li>
+  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol2#protocol_2" style="text-decoration: none""><font color="#0080FF" size="3">Growing the Single Colonies from the Agar Plates</font></a> </li>
-  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_3#Protocol_3" style="text-decoration: none""><font
+  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_3#protocol_3" style="text-decoration: none""><font
 color="#0080FF" size="3"> Making glycerol stocks</font></a> </li>
-  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_4#Protocol_4" style="text-decoration: none""><font
+  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_4#protocol_4" style="text-decoration: none""><font
-color="#0080FF" size="3"> Miniprep Protocol</font></a> </li>
+color="#0080FF" size="3"> Plasmid Purification Protocol</font></a> </li>
-  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_5#Protocol_5" style="text-decoration: none""><font
+  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_5#protocol_5" style="text-decoration: none""><font
 color="#0080FF" size="3"> Restriction digestion</font></a> </li>
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-  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_6#Protocol_6" style="text-decoration: none""><font
+  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_6#protocol_6" style="text-decoration: none""><font
 color="#0080FF" size="3"> Ligation</font></a> </li>
-  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_7#Protocol_7" style="text-decoration: none"" ><font
+  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_7#protocol_7" style="text-decoration: none"" ><font
 color="#0080FF" size="3"> Gel Extraction Procedure</font></a> </li>
-  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_8#Protocol_8" style="text-decoration: none""><font
+  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_8#protocol_8" style="text-decoration: none""><font
 color="#0080FF" size="3">  PCR Purification</font></a> </li>
-  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_9#Protocol_9" style="text-decoration: none""><font
+  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_9#protocol_9" style="text-decoration: none""><font
 color="#0080FF" size="3">  Tricine Gels</font></a> </li>
-   <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_10#general" style="text-decoration: none""><font color="#0080FF" size="3">General Peptide Production</font></a> </li>
+   <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_10#protocol_10" style="text-decoration: none""><font color="#0080FF" size="3">General Peptide Production</font></a> </li>
+  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_11#protocol_11" style="text-decoration: none""><font color="#0080FF" size="3">SUMO cleavage</font></a> </li>
+  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_12#protocol_12" style="text-decoration: none""><font color="#0080FF" size="3">Lysis Protocol</font></a> </li>
+  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_13#protocol_13" style="text-decoration: none""><font color="#0080FF" size="3">AIP Sensing Protocol</font></a> </li>
+  <li> <a href="https://static.igem.org/mediawiki/2013/6/6b/Gene_synthesis.pdf" target="blank" style="text-decoration: none""><font color="#0080FF" size="3">Gene Design</font></a> </li>
+  <li> <a href="https://static.igem.org/mediawiki/2013/3/3f/Primers.pdf" target="blank" style="text-decoration: none""><font color="#0080FF" size="3">Primer Design</font></a> </li>
 </ul>