Team:TU-Delft/ProtocolList
From 2013.igem.org
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<h2 align="center">Protocols</h2> | <h2 align="center">Protocols</h2> | ||
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<li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_4#protocol_4" style="text-decoration: none""><font | <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_4#protocol_4" style="text-decoration: none""><font | ||
- | color="#0080FF" size="3"> | + | color="#0080FF" size="3"> Plasmid Purification Protocol</font></a> </li> |
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color="#0080FF" size="3"> Tricine Gels</font></a> </li> | color="#0080FF" size="3"> Tricine Gels</font></a> </li> | ||
- | <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_10# | + | <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_10#protocol_10" style="text-decoration: none""><font color="#0080FF" size="3">General Peptide Production</font></a> </li> |
- | <li> <a href="https://2013.igem.org/Team:TU-Delft/ | + | <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_11#protocol_11" style="text-decoration: none""><font color="#0080FF" size="3">SUMO cleavage</font></a> </li> |
- | <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_12#protocol_12" style="text-decoration: none""><font color="#0080FF" size="3">Lysis</font></a> </li> | + | <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_12#protocol_12" style="text-decoration: none""><font color="#0080FF" size="3">Lysis Protocol</font></a> </li> |
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+ | <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_13#protocol_13" style="text-decoration: none""><font color="#0080FF" size="3">AIP Sensing Protocol</font></a> </li> | ||
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+ | <li> <a href="https://static.igem.org/mediawiki/2013/6/6b/Gene_synthesis.pdf" target="blank" style="text-decoration: none""><font color="#0080FF" size="3">Gene Design</font></a> </li> | ||
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+ | <li> <a href="https://static.igem.org/mediawiki/2013/3/3f/Primers.pdf" target="blank" style="text-decoration: none""><font color="#0080FF" size="3">Primer Design</font></a> </li> | ||
</ul> | </ul> |
Latest revision as of 20:38, 4 October 2013
Protocols
Our project deals with E.coli cells which sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and starts producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Different protocols used during the project are described below.
- Transforming Parts from Distribution kit
- Growing the Single Colonies from the Agar Plates
- Making glycerol stocks
- Plasmid Purification Protocol
- Restriction digestion
- Ligation
- Gel Extraction Procedure
- PCR Purification
- Tricine Gels
- General Peptide Production
- SUMO cleavage
- Lysis Protocol
- AIP Sensing Protocol
- Gene Design
- Primer Design