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Team:Paris Saclay/Notebook/August/23
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==='''A - Aerobic/Anaerobic regulation system'''=== | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
| - | ===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in | + | ===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in pSB3K3'''==== |
===='''1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI'''==== | ===='''1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI'''==== | ||
{| | {| | ||
| - | | style="width:350px;border:1px solid black;" |[[File:Psgel2308.jpg]] | + | | style="width:350px;border:1px solid black;" |[[File:Psgel2308.jpg|200px]] |
| style="width:350px;border:1px solid black;vertical-align:top;" | | | style="width:350px;border:1px solid black;vertical-align:top;" | | ||
* Well 1 : 6µL DNA Ladder | * Well 1 : 6µL DNA Ladder | ||
| - | * Well 2 : 5µL of BBa_J04450 digested by EcoRI/PstI+ | + | * Well 2 : 5µL of BBa_J04450 digested by EcoRI/PstI +1 µl of 6X loading dye |
* Gel : 1% | * Gel : 1% | ||
|} | |} | ||
Expect sizes : | Expect sizes : | ||
| - | * | + | * pSB3K3 : 2750 bp |
{| | {| | ||
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| - | We can see anything. The digestion | + | We can't see anything. The digestion didn't work. |
|} | |} | ||
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|[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]] | |[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]] | ||
| - | |[[Team:Paris Saclay/Notebook/August/ | + | |[[Team:Paris Saclay/Notebook/August/26|<big>Next day</big>]] |
|} | |} | ||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} | ||
Latest revision as of 01:21, 5 October 2013
Contents |
Notebook : August 23
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in pSB3K3
1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI
|
|
Expect sizes :
- pSB3K3 : 2750 bp
|
We can't see anything. The digestion didn't work. |
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