Latest revision as of 20:01, 27 October 2013

Collaboration

All of the work that was done during this project was both planned and executed by our iGEM team members. It all started of with some brainstorming sessions that began in the middle of May, although the actual lab work began in the beginning of June.

During the summer we have worked very closely with the Linköpings iGEM team 2013 . Since it was their first year to participate in the competition we decided to do our best to provide them with any assistance they needed, such as providing them with parts, knowledge etc.

In addition to assisting Linköpings iGEM team we have also sent biobricks to the following iGEM teams:

iGEM 2013 UT-Tokyo:
BBa_K592010 - amilGFP, yellow chromoprotein
BBa_K864401 - aeBlue, blue chromoprotein

iGEM 2013 Braunschweig:
BBa_K1033930 - amilCP, blue/purple chromoprotein (incl RBS)
BBa_K592010 - amilGFP, yellow chromoprotein
BBa_K592012 - eforRed, red/pink chromoprotein
BBa_K592011 - cjBlue, green chromoprotein
BBa_K864401 - aeBlue, blue chromoprotein

iGEM 2013 Tsinghua:
BBa_K592010 - amilGFP, yellow chromoprotein
BBa_K592012 - eforRed, red/pink chromoprotein
BBa_K592011 - cjBlue, green chromoprotein
BBa_K864401 - aeBlue, blue chromoprotein
BBa_K1033930 - amilCP, blue/purple chromoprotein (incl RBS)

iGEM 2013 CU Boulder:
BBa_K592010 - amilGFP, yellow chromoprotein
BBa_K592012 - eforRed, red/pink chromoprotein
BBa_K592011 - cjBlue, green chromoprotein
BBa_K864401 - aeBlue, blue chromoprotein
BBa_K1033930 - amilCP, blue/purple chromoprotein (incl RBS)

iGEM 2013 uOttawa:
BBa_K592010 - amilGFP, yellow chromoprotein
BBa_K592012 - eforRed, red/pink chromoprotein

iGEM 2013 HokkaidoU:
BBa_K592009 - amilCP, blue chromoprotein (incl RBS)
BBa_K1033931 - amilGFP, yellow chromoprotein (incl RBS)
BBa_K592010 - amilGFP, yellow chromoprotein
BBa_K592012 - eforRed, red/pink chromoprotein
BBa_K592011 - cjBlue, green chromoprotein
BBa_K864401 - aeBlue, blue chromoprotein

iGEM 2013 TU Darmstadt:
BBa_K592012 - eforRed, red/pink chromoprotein
BBa_K592011 - cjBlue, green chromoprotein
BBa_K1033929 - aeBlue, blue chromoprotein (incl RBS)
BBa_K1033928 - aeBlue, blue chromoprotein (incl RBS, J23110)
BBa_K1033903 - tsPurple, purple chromoprotein (incl RBS, J23110)
BBa_K1033930 - amilCP, blue/purple chromoprotein (incl RBS)
BBa_K1033931 - amilGFP, yellow chromoprotein (incl RBS)

A special thanks

We would also like to thank other sources that have helped us, such as the Slovenian iGEM team 2010 that supplied us with the genes needed for the production of carotenoid metabolites, TU-Munichs iGEM team 2012 that provided us with the gene 4CL for the production of 4-coumaryl-CoA, and Wahington University's 2012 iGEM team WashU that enabled our work with the saffron pathway. Last but not least we would also like to thank the School of chemical and Biomolecular Engineering, Cornell University for providing us with the genes involved in the production of the phenylpropanoid pathway. The article can be found at; Conrado RJ, Mori J et al (2011)

Other collaborations

In addition to this we would also like to mention some fascinating requests that have been made by external sources:

Nature reviews request to display our chromoproteins on their front page for their 10 year anniversary.
National Aeronautics and Space Administrations (NASA) biotechnology department requested our chromoproteins as reporter genes for a project that is currently being worked on.

@@ Line 13: / Line 13: @@
 </style>
+<script type="text/javascript">
+/*Acts once the document is loaded*/ $(document).ready(function() { /*Move the tn-main-wrap as child of <body>*/ $("#tn-main-wrap-wrap").prependTo($("body")); /*Move the menubars in the tn-main-wrap*/ $("#menubar.left-menu").appendTo($("#tn-main-wrap")); $("#menubar.right-menu").appendTo($("#tn-main-wrap"));
+/*Spoiler JS*/ $(".tn-spoiler div").slideUp(); $(".tn-spoiler a").click(function(e) { e.preventDefault(); $(".tn-spoiler-active").removeClass("tn-spoiler-active"); $(this).parent().addClass("tn-spoiler-active"); $(".tn-spoiler").not(".tn-spoiler-active").children("div").slideUp(); $(".tn-spoiler-active").children("div").slideToggle(); });
+/*Add favicon*/ $("link[rel='shortcut icon']").remove(); $("head").append("<link rel='shortcut icon' type='image/png' href='https://static.igem.org/mediawiki/2013/6/6d/Uppsalas-cow-con.png'>"); });
+</script>
 </head>
@@ Line 30: / Line 36: @@
 			<div id="igem"><a href="https://2013.igem.org/Main_Page"><img class="igem" src="https://static.igem.org/mediawiki/2013/b/b8/Uppsala2013_Uppsala_IGEM_log_blue.png"></a></div>
+<img class="slogan" src="https://static.igem.org/mediawiki/2013/a/ad/Uppsala2013_Header.png">
 	</div>
@@ Line 57: / Line 65: @@
                                                  <li><a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering">Metabolic engineering</a>
                                                      <ul>
-                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid">P-coumaric acid</a></li>
+                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid">p-Coumaric acid</a></li>
                                                                  <li><a href="https://2013.igem.org/Team:Uppsala/resveratrol">Resveratrol</a></li>
 								<li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li>
@@ Line 78: / Line 86: @@
 				<li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/6/63/Uppsala2013_Modeling.png"></a>
 					<ul>
-						<li><a href="https://2013.igem.org/Team:Uppsala/P-Coumaric-acid-pathway">P-Coumaric acid</a></li>
+						<li><a href="https://2013.igem.org/Team:Uppsala/P-Coumaric-acid-pathway">Kinetic model</a></li>
 						<li><a href="https://2013.igem.org/Team:Uppsala/modeling-tutorial">Modeling tutorial </a></li>
+<li><a href="https://2013.igem.org/Team:Uppsala/toxicity-model">Toxicity model</a></li>
 					</ul></li>
 				<li><a href="https://2013.igem.org/Team:Uppsala/parts" id="list_type2"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/e/eb/Uppsala2013_parts.png"></a></li>
@@ Line 88: / Line 98: @@
 						<li><a href="https://2013.igem.org/Team:Uppsala/carotenoid-group">Carotenoid group</a></li>
 						<li><a href="https://2013.igem.org/Team:Uppsala/chassi-group">Chassi group</a></li>
+                                                <li><a href="https://2013.igem.org/Team:Uppsala/advisors">Advisors</a></li>
 					</ul></li>
@@ Line 97: / Line 108: @@
 						<li><a href="https://2013.igem.org/Team:Uppsala/public-opinion">Public opinion </a></li>
                                                  <li><a href="https://2013.igem.org/Team:Uppsala/Outreach">High school & media </a></li>
+<li><a href="https://2013.igem.org/Team:Uppsala/bioart">BioArt</a></li>
+<li><a href="https://2013.igem.org/Team:Uppsala/LactonutritiousWorld">A LactoWorld</a></li>
+<li><a href="https://2013.igem.org/Team:Uppsala/killswitches">Killswitches</a></li>
+<li><a href="https://2013.igem.org/Team:Uppsala/realization">Patent</a></li>
 					</ul></li>
-				<li><a href="https://2013.igem.org/Team:Uppsala/attribution" id="list_type4"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/5/5d/Uppsala2013_Attributions.png"></a>
+				<li><a href="https://2013.igem.org/Team:Uppsala/attribution" id="list_type4"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/5/5d/Uppsala2013_Attributions.png"></a></li>
-                                <ul>
-                                    <li><a href="https://2013.igem.org/Team:Uppsala/collaboration">Collaboration</a></li>
-                                </ul></li>
 				<li><a href="https://2013.igem.org/Team:Uppsala/notebook" id="list_type3"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/3/36/Uppsala2013_Notebook.png"></a>
                                       <ul>
                                          <li><a href="https://2013.igem.org/Team:Uppsala/safety-form">Safety form</a></li>
+                                        <li><a href="https://2013.igem.org/Team:Uppsala/protocols">Protocols</a></li>
                                       </ul></li>
 				</ul>
@@ Line 122: / Line 134: @@
 <br>
-During the summer we have worked very closely with the Linköpings iGEM team. Since it was their first year to participate in the competition we decided to do our best to provide them with any assistance they needed, such as providing them with parts, knowledge etc. In exchange, they provided us with some very valuable information about basic modeling that we could use when modeling our own system.
+During the summer we have worked very closely with the <a href="https://2013.igem.org/Team:Linkoping_Sweden"> Linköpings iGEM team 2013 </a>. Since it was their first year to participate in the competition we decided to do our best to provide them with any assistance they needed, such as providing them with parts, knowledge etc.
 <br>
@@ Line 128: / Line 140: @@
 In addition to assisting Linköpings iGEM team we have also sent biobricks to the following iGEM teams:
-<br>
 <br>
 <br>
-UT-Tokyo iGEM:<br>
+ iGEM 2013 <a href="https://2013.igem.org/Team:UT-Tokyo"> UT-Tokyo: </a> <br>
-<!--
+<a href="http://parts.igem.org/Part:BBa_K592010"> BBa_K592010 </a> - amilGFP, yellow chromoprotein <br>
-BBa_K592010	pSB1C3-amilGFP
+<a href="http://parts.igem.org/Part:BBa_K864401">  BBa_K864401 </a> - aeBlue, blue chromoprotein <br>
-BBa_K864401	pSB1C3-aeBlue
--->
-<br>
-<br>
 <br>
-iGEM Braunschweig:<br>
+iGEM 2013 <a href="https://2013.igem.org/Team:Braunschweig"> Braunschweig: </a> <br>
-<!--
+<a href="http://parts.igem.org/Part:BBa_K1033930">BBa_K1033930</a> - amilCP, blue/purple chromoprotein (incl RBS) <br>
-BBa_K592010	pSB1C3-amilGFP
+<a href="http://parts.igem.org/Part:BBa_K592010"> BBa_K592010 </a> - amilGFP, yellow chromoprotein <br>
-BBa_K592011	pSB1C3-cjBlue
+<a href="http://parts.igem.org/Part:BBa_K592012"> BBa_K592012 </a> - eforRed, red/pink chromoprotein <br>
-BBa_K592012     pSB1C3-eforRed
+<a href="http://parts.igem.org/Part:BBa_K592011"> BBa_K592011 </a> - cjBlue, green chromoprotein<br>
-BBa_K592025     pSB1C3-B0034-amilCP
+<a href="http://parts.igem.org/Part:BBa_K864401">  BBa_K864401 </a> - aeBlue, blue chromoprotein <br>
-BBa_K864401     pSB1C3-aeBlue
--->
-<br>
-<br>
 <br>
-Tsinghua iGEM 2013: <br>
+iGEM 2013 <a href="https://2013.igem.org/Team:Tsinghua"> Tsinghua: </a>  <br>
-<!--
+<a href="http://parts.igem.org/Part:BBa_K592010"> BBa_K592010 </a> - amilGFP, yellow chromoprotein <br>
-BBa_K592010           pSB1C3-amilGFP
+<a href="http://parts.igem.org/Part:BBa_K592012"> BBa_K592012 </a> - eforRed, red/pink chromoprotein <br>
-BBa_K592011           pSB1C3-cjBlue
+<a href="http://parts.igem.org/Part:BBa_K592011"> BBa_K592011 </a> - cjBlue, green chromoprotein<br>
-BBa_K592012           pSB1C3-eforRed
+<a href="http://parts.igem.org/Part:BBa_K864401">  BBa_K864401 </a> - aeBlue, blue chromoprotein <br>
-BBa_K592025           pSB1C3-B0034-amilCP
+<a href="http://parts.igem.org/Part:BBa_K1033930">BBa_K1033930</a> - amilCP, blue/purple chromoprotein (incl RBS) <br>
-BBa_K864401           pSB1C3-aeBlue
--->
-<br>
-<br>
 <br>
-CU Boulder iGEM:<br>
+iGEM 2013 <a href="https://2013.igem.org/Team:CU-Boulder"> CU Boulder: </a> <br>
-<!--
+<a href="http://parts.igem.org/Part:BBa_K592010"> BBa_K592010 </a> - amilGFP, yellow chromoprotein <br>
-BBa_K592010	pSB1C3-amilGFP
+<a href="http://parts.igem.org/Part:BBa_K592012"> BBa_K592012 </a> - eforRed, red/pink chromoprotein <br>
-BBa_K592011	pSB1C3-cjBlue
+<a href="http://parts.igem.org/Part:BBa_K592011"> BBa_K592011 </a> - cjBlue, green chromoprotein<br>
-BBa_K592012	pSB1C3-eforRed
+<a href="http://parts.igem.org/Part:BBa_K864401">  BBa_K864401 </a> - aeBlue, blue chromoprotein <br>
-BBa_K592025	pSB1C3-B0034-amilCP
+<a href="http://parts.igem.org/Part:BBa_K1033930">BBa_K1033930</a> - amilCP, blue/purple chromoprotein (incl RBS) <br>
-BBa_K864401	pSB1C3-aeBlue
--->
-<br>
-<br>
 <br>
-uOttawa<br>
+iGEM 2013 <a href="https://2013.igem.org/Team:uOttawa"> uOttawa: </a>  <br>
-<!--
+<a href="http://parts.igem.org/Part:BBa_K592010"> BBa_K592010 </a> - amilGFP, yellow chromoprotein <br>
-yellow chromoprotein: BBa_K592010
+<a href="http://parts.igem.org/Part:BBa_K592012"> BBa_K592012 </a> - eforRed, red/pink chromoprotein <br>
-red chromoproteins: BBa_K592012
--->
-<br>
-<br>
 <br>
-HokkaidoU <br>
+iGEM 2013 <a href="https://2013.igem.org/Team:HokkaidoU_Japan"> HokkaidoU: </a> <br>
-<!--
+<a href="http://parts.igem.org/Part:BBa_K592009"> BBa_K592009 </a> - amilCP, blue chromoprotein (incl RBS) <br>
-K592009 – AmilCP (with RBS)
+<a href="http://parts.igem.org/Part:BBa_K1033931">BBa_K1033931</a> - amilGFP, yellow chromoprotein (incl RBS) <br>
-K592010 – AmilGFP (with and without RBS)
+<a href="http://parts.igem.org/Part:BBa_K592010"> BBa_K592010 </a> - amilGFP, yellow chromoprotein <br>
-K592011 – CjBlue (only without RBS)
+<a href="http://parts.igem.org/Part:BBa_K592012"> BBa_K592012 </a> - eforRed, red/pink chromoprotein <br>
-K592012 – eforRed (only without RBS)
+<a href="http://parts.igem.org/Part:BBa_K592011"> BBa_K592011 </a> - cjBlue, green chromoprotein<br>
-K864401 – aeBlue (only without RBS)
+<a href="http://parts.igem.org/Part:BBa_K864401">  BBa_K864401 </a> - aeBlue, blue chromoprotein <br>
--->
-<br>
-<br>
 <br>
-TU Darmstadt <br>
+iGEM 2013 <a href="https://2013.igem.org/Team:TU_Darmstadt"> TU Darmstadt: </a>  <br>
-<!--
+<a href="http://parts.igem.org/Part:BBa_K592012"> BBa_K592012 </a> - eforRed, red/pink chromoprotein <br>
-pSB1C3-eforRed
+<a href="http://parts.igem.org/Part:BBa_K592011"> BBa_K592011 </a> - cjBlue, green chromoprotein<br>
-pSB1C3-CjBlue
+<a href="http://parts.igem.org/Part:BBa_K1033929">BBa_K1033929</a> - aeBlue, blue chromoprotein (incl RBS) <br>
-pSB1C3-B0034-aeBlue
+<a href="http://parts.igem.org/Part:BBa_K1033928">BBa_K1033928</a> - aeBlue, blue chromoprotein (incl RBS, J23110) <br>
-pSB1K3-J23110-B0034-aeBlue
+<a href="http://parts.igem.org/Part:BBa_K1033903"> BBa_K1033903 </a> - tsPurple, purple chromoprotein (incl RBS, J23110) <br>
-pSB1C3-B0034-amilGFP
+<a href="http://parts.igem.org/Part:BBa_K1033930">BBa_K1033930</a> - amilCP, blue/purple chromoprotein (incl RBS) <br>
-pSB1C3-B0034-amilCP
+<a href="http://parts.igem.org/Part:BBa_K1033931">BBa_K1033931</a> - amilGFP, yellow chromoprotein (incl RBS) <br>
--->
-<a href="http://parts.igem.org/Part:BBa_K1033903">BBa_K1033903</a> - tsPurple, purple chromoprotein (incl RBS, J23110) <br>
 <br>
-<br>
-<br>
-We would also like to thank other sources that have helped us, such as the Slovenian iGEM team 2010 that suppied us with the genes needed for the production of carotenoid metabolites, TU-Munichs iGEM team 2012 that provided us with the gene 4CL for the production of 4-coumaryl-CoA, last but not least we would also like to thank the School of chemical and Biomolecular Engineering, Cornell University for providing us with the genes involved in the production of the phenylpropanoid pathway. The article can be found at; Conrado RJ, Mori J et al (2011)
+<h1> A special thanks </h1>
-<br>
+We would also like to thank other sources that have helped us, such as the <a href="https://2010.igem.org/Team:Slovenia"> Slovenian iGEM team 2010 </a> that supplied us with the genes needed for the production of carotenoid metabolites, <a href="https://2012.igem.org/Team:TU_Munich"> TU-Munichs iGEM team 2012 </a> that provided us with the gene 4CL for the production of 4-coumaryl-CoA, and <a href= "https://2012.igem.org/Team:WashU"> Wahington University's 2012 iGEM team WashU </a> that enabled our work with the saffron pathway. Last but not least we would also like to thank the School of chemical and Biomolecular Engineering, Cornell University for providing us with the genes involved in the production of the phenylpropanoid pathway. The article can be found at; <a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287197/pdf/gkr888.pdf"> Conrado RJ, Mori J et al (2011) </a>
 <br>
 <br>
+<h1> Other collaborations </h1>
 In addition to this we would also like to mention some fascinating requests that have been made by external sources:
-<li> Nature reviews request to display our chromoproteins on their front page for their 10 year anniversary </li>
+<ul>
+<li> Nature reviews request to display our chromoproteins on their <a href="http://www.nature.com/nrmicro/journal/v11/n10/covers/index.html"> front page </a> for their 10 year anniversary. </li>
 <li>National Aeronautics and Space Administrations (NASA) biotechnology department requested our chromoproteins as reporter genes for a project that is currently being worked on. </li>
+</ul>
@@ Line 235: / Line 224: @@
 	<div id="bottom-pic">
-		<img class="bottom-pic" src="https://static.igem.org/mediawiki/2013/a/aa/Bottom_picture.png">
+			</div>
-	</div>