Team:TU-Munich/Results/Overview

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== Results Overview ==
== Results Overview ==
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Until the regional jamborre in Lyon we were able to create xx BioBricks and devices, have successfully transformed and selected xx trangenic GM-mosses. Different effectors were characterized as a recombinant protein which were produced in ''E. coli'' but also as transformation in our moss ''Physcomitrella patens''. Beside these activities we contributed to the progress of the iGEM community by writing a software tool for the annotation of BioBricks and we also wrote tutorials to conserve the knowledge we have gathered during this summer for subsequent generation of iGEM students. Furthermore we developed a concept based on swimming triangular pods how our PhyscoFilter could be implemented into rivers and analyzed the economic potential of this innovative technology in our Entrepreneurship section.
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Having spent our summer in the lab, we are proud to present our accomplishments. Up to the European regional jamboree in Lyon, we have created 72 BioBricks and devices, transformed and selected 20 different transgenic GM-mosses and characterized our effectors as recombinant proteins produced in ''E. coli'' and in our moss ''Physcomitrella patens'', a chassis newly introduced into iGEM. We took further steps to put our phytoremediation project into practice by developing concepts for the implementation of our PhyscoFilter in the environment and by analyzing the economic potential of this innovative technology in our Entrepreneurship section. Additional to our wetlab work, we contributed a very powerful software tool for the annotation of BioBricks from the Parts Registry and created tutorials to pass this summer´s experiences and skills on to the subsequent iGEM generations.  
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<div class="box-left overview"><html><img src="https://static.igem.org/mediawiki/2013/d/dc/TUM13_results-1.jpg" /></html>
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===BioBricks===
===BioBricks===
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[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 1:]]
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We created 72 BioBricks, including BioBricks advancing the use of ''Physcomitrella patens'' as a chassis, BioBricks enhancing phytoremediation applications and BioBricks for the light triggered kill-switch mechanism.<br>
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During the summer the team has created xx BioBricks which can mainly be grouped into BioBricks enabling ''Physcomitrella patens'' as chassis, Phytoremediation BioBricks and a light triggered kill-switch (https://2013.igem.org/Team:TU-Munich/Results/BioBricks Read more]).</div>
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[https://2013.igem.org/Team:TU-Munich/Results/BioBricks Read more]
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<div class="box-right overview"><html><img src="https://static.igem.org/mediawiki/2013/8/80/TUM13_results-2.jpg" /></html>
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===Effectors===
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===Effector studies===
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[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 2:]]
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We selected six different effector proteins, produced them in ''Escherichia coli'' and characterized them, concerning stability and activity.<br>
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We chose for six different effector proteins which we have produced recombinantly in ''E. coli'' to characterize them on a molecular level concerning stability and activity ([https://2013.igem.org/Team:TU-Munich/Results/Recombinant Read more]).
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[https://2013.igem.org/Team:TU-Munich/Results/Recombinant Read more]
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<div class="box-left overview"><html><img src="https://static.igem.org/mediawiki/2013/e/ee/TUM13_results-3.jpg" /></html>
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===Moss Methods===
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We performed some general experi- ments to optimize  ''Physcomitrella patens'' concerning tolerance of toxins, growth optimization and the use of different cultivation surfaces. Furthermore, we created 20 different strains of transformed moss during our visit to Prof. Reski's lab in Freiburg. [https://2013.igem.org/Team:TU-Munich/Results/Moss Read more]
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===Moss Experiments===
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[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 3:]]
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As at our university no research group works with ''Physcomitrella patens'' we had to make some general experiment with this moss concerning tolerace of toxin, growing properties on different surfaces and the determination of growth rates ([https://2013.igem.org/Team:TU-Munich/Results/Moss Read more]).
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<div class="box-right overview"><html><img src="https://static.igem.org/mediawiki/2013/e/e0/TUM13_Receptor_small.png" /></html>
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===GM-Moss===
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===Localization===
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[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 4:]]
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In order to ensure the best functionality for our effectors, we designed and used several localization methods as well as different verification techniques to see if our localizations were successful.
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In our project we created 25 different strains of stabily transformated moss. This was done 350 km away in Freiburg and we then selected and regenerated transgenic moss over 4 weeks and tested several of them in 96-well plates (https://2013.igem.org/Team:TU-Munich/Results/GM-Moss Read more]).
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<br>[https://2013.igem.org/Team:TU-Munich/Results/Localization Read more]
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<div class="box-left overview"><html><img src="https://static.igem.org/mediawiki/2013/2/2a/TUM13_results-4.jpg" /></html>
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===PhyscoFilter===
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After the selection and regeneration of the transgenic moss plants, we performed experiments with them and characterized their properties as PhyscoFilter. <br>[https://2013.igem.org/Team:TU-Munich/Results/GM-Moss Read more]
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===Software===
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[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 5:]]
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Protein coding BioBricks are an important class in the Parts Registry. As they are standardized by definition our AutoAnnotator translated the sequence computes parameters, does alignments and gives a formated table as output (https://2013.igem.org/Team:TU-Munich/Results/Software Read more]).
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<div class="box-right overview"><html><img src="https://static.igem.org/mediawiki/2013/6/63/TUM13_project-6.jpg" /></html>
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===Tutorials===
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===Kill Switch===
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[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 6:]]
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In order to prevent uncontrolled growth of transgenic moss in the environment, we developed a Kill-Switch which is triggered by sunlight. The GM-Moss can only be grown where red-light is filtered out of the electromagnetic spectrum. <br>[https://2013.igem.org/Team:TU-Munich/Results/KillSwitch Read more]
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During the summer we learned a lot about iGEM and found solutions for problems which appear in many iGEM projects. So we want to share this knowledge for the benefit of the community. Therefore we created our tutorial section ([https://2013.igem.org/Team:TU-Munich/Results/How_To Read more]).
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<div class="box-left overview"><html><img src="https://static.igem.org/mediawiki/2013/6/6d/TUM13_project-8.jpg" /></html>
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===Implementation===
===Implementation===
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[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 6:]]
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The creation of new environmental solutions doesn't stop at the development of GM moss. We tried to find out how a large-scale biofilter could be implemented.<br>[https://2013.igem.org/Team:TU-Munich/Results/Implementation Read more]
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As soon as transgenic moss with an phytoremediation activity is available it becomes necessary to develop a solution of how to implement a SynBio waterfilter into the environment. For this purpose we present a concept of swimming triangular pods (https://2013.igem.org/Team:TU-Munich/Project/Implementation Read more]).
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<div class="box-right overview"><html><img src="https://static.igem.org/mediawiki/2013/b/bc/TUM13_results-5.jpg" /></html>
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===AutoAnnotator===
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Protein coding BioBricks constitute large parts of the Parts Registry. We created a software tool for in-silico characterization of various para- meters summed up in a standardized table. To improve the parts registry, <nowiki>RFC 96</nowiki> proposes a range of characteristics determined by the AutoAnnotator.
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[https://2013.igem.org/Team:TU-Munich/Results/Software Read more]
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<div class="box-left overview"><html><img src="https://static.igem.org/mediawiki/2013/e/e6/TUM13_results-7.jpg" /></html>
===Entrepreneurship===
===Entrepreneurship===
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[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 7:]]
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To translate science into applied technology, available to the public, economic and business factors play increasingly important roles. We took the first steps into this direction by examining criteria for implementation and possibilities of business models in biotechnology.<br> [https://2013.igem.org/Team:TU-Munich/Results/Economics Read more]
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Science is an extremely important point for progress but in the end it is essential to developp a business model oppon which a the technology can be brought to market ([https://2013.igem.org/Team:TU-Munich/Results/Economics Read more]).
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Latest revision as of 03:43, 29 October 2013


Results Overview

Having spent our summer in the lab, we are proud to present our accomplishments. Up to the European regional jamboree in Lyon, we have created 72 BioBricks and devices, transformed and selected 20 different transgenic GM-mosses and characterized our effectors as recombinant proteins produced in E. coli and in our moss Physcomitrella patens, a chassis newly introduced into iGEM. We took further steps to put our phytoremediation project into practice by developing concepts for the implementation of our PhyscoFilter in the environment and by analyzing the economic potential of this innovative technology in our Entrepreneurship section. Additional to our wetlab work, we contributed a very powerful software tool for the annotation of BioBricks from the Parts Registry and created tutorials to pass this summer´s experiences and skills on to the subsequent iGEM generations.

BioBricks

We created 72 BioBricks, including BioBricks advancing the use of Physcomitrella patens as a chassis, BioBricks enhancing phytoremediation applications and BioBricks for the light triggered kill-switch mechanism.
Read more

Effector studies

We selected six different effector proteins, produced them in Escherichia coli and characterized them, concerning stability and activity.
Read more

Moss Methods

We performed some general experi- ments to optimize Physcomitrella patens concerning tolerance of toxins, growth optimization and the use of different cultivation surfaces. Furthermore, we created 20 different strains of transformed moss during our visit to Prof. Reski's lab in Freiburg. Read more

Localization

In order to ensure the best functionality for our effectors, we designed and used several localization methods as well as different verification techniques to see if our localizations were successful.
Read more

PhyscoFilter

After the selection and regeneration of the transgenic moss plants, we performed experiments with them and characterized their properties as PhyscoFilter.
Read more

Kill Switch

In order to prevent uncontrolled growth of transgenic moss in the environment, we developed a Kill-Switch which is triggered by sunlight. The GM-Moss can only be grown where red-light is filtered out of the electromagnetic spectrum.
Read more

Implementation

The creation of new environmental solutions doesn't stop at the development of GM moss. We tried to find out how a large-scale biofilter could be implemented.
Read more

AutoAnnotator

Protein coding BioBricks constitute large parts of the Parts Registry. We created a software tool for in-silico characterization of various para- meters summed up in a standardized table. To improve the parts registry, RFC 96 proposes a range of characteristics determined by the AutoAnnotator. Read more

Entrepreneurship

To translate science into applied technology, available to the public, economic and business factors play increasingly important roles. We took the first steps into this direction by examining criteria for implementation and possibilities of business models in biotechnology.
Read more

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