Team:Evry/Achievements
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<h1>iGEM Evry 2013 achievements</h1> | <h1>iGEM Evry 2013 achievements</h1> | ||
<br> | <br> | ||
- | |||
- | < | + | <div class="jamboree"> |
+ | <header>Since European Jamboree!</header> | ||
+ | <p> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/7/71/IGEM_Lyon.png" width=200px style="padding: 5px;"/> | ||
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+ | <table> | ||
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+ | <td colspan=2> | ||
+ | <p>We worked hard since the last european Jamboree, taking into account the remarks from the juges and continuing on our own ideas. Here are the our main acheivements:<p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td width=75px style="vertical-align:top;" > | ||
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+ | <img src="https://static.igem.org/mediawiki/2013/1/1a/New.png"> | ||
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+ | </td> | ||
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+ | <p> | ||
+ | <br /> | ||
<ul> | <ul> | ||
- | <li> | + | <li><b>Biology:</b></li> |
- | <li> | + | <ul> |
- | <li | + | <li>Proceed to new inverter constructions</li> |
+ | <li>Cloned an periplasm located iron chelating protein coming from <i>Nesseria</i></li> | ||
+ | </ul> | ||
+ | <li><b>Modeling:</b></li> | ||
+ | <ul> | ||
+ | <li>We parametrized all our models with the experimental parameters of the iron sensor</li> | ||
+ | <li>We created a cellular automaton based model of the treatment in the upper intestine</li> | ||
+ | </ul> | ||
+ | <li><b>Human Practice:</b></li> | ||
+ | <ul> | ||
+ | <li>We estimated the cost of the capsule, the benefits for the company producing it and the gain of the French national care system</li> | ||
+ | <li>We consulted SANOFI, a world leader drug maker</li> | ||
+ | </ul> | ||
</ul> | </ul> | ||
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- | < | + | </p> |
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td style="vertical-align:top;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/d/df/Improved.png" align="center" width=81px> | ||
+ | </td> | ||
+ | <td> | ||
<p> | <p> | ||
+ | <br /> | ||
<ul> | <ul> | ||
- | <li | + | <li><b>Biology:</b></li> |
- | <li | + | <ul> |
- | + | <li>We improved the characterization of our best iron sensor</li> | |
- | <ul> | + | </ul> |
- | <li>< | + | <li><b>Modeling:</b></li> |
- | < | + | <ul> |
- | <li>< | + | <li>We reviewed the math, the code and improved the description of all our models for further re-use in iGEM</li> |
- | <li>< | + | </ul> |
- | < | + | <li><b>Human Practice:</b></li> |
- | <li>< | + | <ul> |
- | <li>< | + | <li>We extended our survey of patient to the rest of the world</li> |
- | < | + | </ul> |
- | < | + | <li><b>Wiki:</b></li> |
- | + | <ul> | |
+ | <li>We made a simplified french version of our Wiki that could be accessible for french patients</li> | ||
+ | <li>We survived a second Wiki-freeze but without coffee this time !!</li> | ||
+ | </ul> | ||
+ | |||
</ul> | </ul> | ||
- | < | + | </p> |
+ | </td> | ||
- | < | + | </tr> |
+ | </table> | ||
- | < | + | </td> |
- | < | + | </table> |
- | < | + | |
- | <a href= | + | |
- | + | </div> | |
- | < | + | </p> |
- | < | + | |
- | + | <br /> | |
+ | <br /> | ||
+ | |||
+ | <p> | ||
+ | Here we detail how we fulfilled each of the <a href="https://2013.igem.org/Judging/Awards" target='_blank'><em>iGEM medal requirements</em></a>. | ||
+ | </p> | ||
+ | |||
+ | <h2 align="center"><img title="Gold Medal" src='https://static.igem.org/mediawiki/2013/c/c4/Or-01%281%29.png' alt="Gold Medal" width="7%"/>Gold Medal (requires any one of the following goals)</h2> | ||
<ul> | <ul> | ||
- | <li><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | |
- | < | + | <li class="tick">We improved the function of an existing BioBrick Part or Device. First, we re-characterize the LacO-RFP BioBrick <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_J04450:Experience" target='_blank'><em>BBa_J04450</em></a> (<a href="https://2013.igem.org/Team:Evry/Results#recharacterization_LacO_RFP" target='_blank'><em>Biology Results Section</em></a>) and improve it by integrating it with an aceB-LacI iron sensor (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1163102:Experience" target='_blank'><em>Biobrick BBa_K1163102</em></a>). Together, these Biobricks create a <a href="https://2013.igem.org/Team:Evry/Inverter" target='_blank'><em>Fur Inverter</em></a> (see <a href="https://2013.igem.org/Team:Evry/Results#Inverter" target='_blank'><em>Biology Results Section</em></a>) that activates gene expression in response to iron.</li> |
- | < | + | |
- | <li><a href=" | + | <li class="tick">We <a href="https://2013.igem.org/Team:Evry/Collaborations"><em>collaborated</em></a> with other iGEM teams: we participated to <b>Purdue</b> and <b>UGent</b> online survey and had a nice workshop with <b>Paris-Saclay</b> team. </br> |
- | <li><a href=" | + | We are also setting up a scientific collaboration with the <b>Edimburgh</b> iGEM team that is working on and environmental sensor using the Fur System. </li> |
- | <li><a href=" | + | |
- | <li><a href=" | + | <li class="tick">As our project involves treating a human genetic disorder using genetically-engineered bacteria, it has numerous implications for human health, safety and security, and ethics. To enable safe delivery of therapeutic bacteria to the human intestine, we designed and constructed a <a href="https://2013.igem.org/Team:Evry/Pill_design"target='_blank'><em>polymer-based capsule</em></a>. We also explored safety and ethics consequences of our project as the Human Practices studies: |
- | <li><a href=" | + | <ol> |
- | </ | + | |
- | </ | + | <li > We discussed the medical feasibility and implications of this treatment in a <a href="https://2013.igem.org/Team:Evry/HumanPractice/Expert_consultation" target='_blank'><em>series of interviews and discussions</em></a> with renowned experts on iron overload diseases.</li> |
+ | |||
+ | <li>We carefully analyzed the <a href="https://2013.igem.org/Team:Evry/Safety" target='_blank'><em>safety issues</em></a> and potential consequences of our bacteria using consequence-based decision tree.</li> | ||
+ | |||
+ | <li>We organized and hosted a <a href="https://2013.igem.org/Team:Evry/Seminar" target='_blank'><em>scientific conference</em></a> on iron overload disorders and perceptions of our our project on 18 September 2013. This conference was attended by both medical experts and iron disorder patients.</li> | ||
+ | |||
+ | <li> We prepared and disseminated a <a href="https://2013.igem.org/Team:Evry/HumanPractice/Patients_perceptions" target='_blank'><em> patient perception survey</em></a> about how our treatment could potentially help hemochromatosis patients. We received and analyzed the results from over 250 patients who responded to our survey.</li> | ||
+ | |||
+ | <li>We wrote an extensive report on our <a href="https://2013.igem.org/Team:Evry/Philosophy" target='_blank'><em>conclusions about synthetic biology </em></a>, which is the fruit of our many discussions over the summer.</li> | ||
+ | |||
+ | </ol> | ||
</ul> | </ul> | ||
- | |||
<br/> | <br/> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | </ | + | <h2 align="center"><img title="Silver Medal" src='https://static.igem.org/mediawiki/2013/d/dc/Argent-01.png' alt="Silver Medal" width="7%"/>Silver Medal (requires each of the four following goals)</h2> |
- | < | + | <p> |
+ | <ul> | ||
+ | <li class="tick">We experimentally validated that the aceB-sfGFP biobrick acts as an iron-reponsive biosensor (<a href="https://2013.igem.org/Team:Evry/Results#AceB_GFP" target='_blank'><em>see Biology Results Section</em></a>). | ||
- | < | + | <li class="tick">We document the characterization of our aceB-sfGFP and other BioBricks in "Main Page" section of the Parts Registry. |
+ | <li class="tick">We submitted the following BioBricks parts to the iGEM Parts Registry. The 4 sfGFP constructs function as <a href="https://2013.igem.org/Team:Evry/Sensor" target='_blank'><em>iron sensors</em></a> and the lacI constructs are used to build a <a href="https://2013.igem.org/Team:Evry/Inverter" target='_blank'><em>Fur-based genetic inverter</em></a> that activates gene expression in response to iron. These 8 Biobricks have been <a href="http://parts.igem.org/cgi/dna_transfer/batch_list.cgi?group_id=1475" target='_blank'><em>received and accepted</em></a> by the Registry of Standard Biological Parts. | ||
+ | <small> | ||
<ul> | <ul> | ||
- | + | <li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1163102:Experience" target='_blank'><em>BBa_K1163102</em></a>: AceB-sfGFP | |
- | + | <li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1163103:Experience" target='_blank'><em>BBa_K1163103</em></a>: AceB-lacI | |
- | + | <li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1163105:Experience" target='_blank'><em>BBa_K1163105</em></a>: FepA-sfGFP | |
- | < | + | <li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1163106:Experience" target='_blank'><em>BBa_K1163106</em></a>: FepA-lacI |
+ | <li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1163108:Experience" target='_blank'><em>BBa_K1163108</em></a>: Fes-sfGFP | ||
+ | <li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1163109:Experience" target='_blank'><em>BBa_K1163109</em></a>: Fes-lacI | ||
+ | <li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1163111:Experience" target='_blank'><em>BBa_K1163111</em></a>: YncE-sfGFP | ||
+ | <li><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1163112:Experience" target='_blank'><em>BBa_K1163112</em></a>: YncE-LacI | ||
+ | </ul> | ||
- | + | </small> <li class="tick">We describe how our project has implications for <b><a href='https://2013.igem.org/Team:Evry/Safety' target='_blank'><em>safety</em></a></b> and <b><a href="https://2013.igem.org/Team:Evry/Philosophy" target='_blank'><em>ethics</em></a></b> | |
- | + | ||
</ul> | </ul> | ||
+ | <br/> | ||
- | |||
+ | <h2 align="center"><img title="Bronze Medal" src='https://static.igem.org/mediawiki/2013/b/bc/Bronze-01.png' width="7%" alt="Bronze Medal"/>Bronze Medal (requires each of the five following goals)</h2> | ||
+ | |||
+ | <p> | ||
+ | <ul> | ||
+ | <li class="tick">We <a href="https://igem.org/Team_List?year=2013" target='_blank'><em> registered our team</em></a> for iGEM 2013 and are attending the European Regional Jamboree. | ||
+ | <li class="tick">We completed and submitted the <a href='https://igem.org/2013_Judging_Form?id=1163' target='_blank'><em>Judging form</em></a>. | ||
+ | <li class="tick">We made a comprehensive and accessible <a href="https://2013.igem.org/Team:Evry" target='_blank'><em>iGEM Evry 2013 Wiki</em></a> to convey our educational and scientific advances. | ||
+ | <li class="tick">We will present a poster and give an oral presentation at the iGEM European Jamboree in Lyon, France.</b> | ||
+ | <li class="tick">We <a href='https://2013.igem.org/Team:Evry/Parts' target='_blank'><em>document 22 BioBricks</em></a></b> used in our project and submit 8 news BioBricks to the Parts Registry (see 8 submitted Biobricks in Silver Medal Requirements). | ||
+ | </ul> | ||
+ | |||
+ | <br/> | ||
+ | <br/> | ||
</div> | </div> |
Latest revision as of 03:53, 29 October 2013
iGEM Evry 2013 achievements
|
Here we detail how we fulfilled each of the iGEM medal requirements.
Gold Medal (requires any one of the following goals)
- We improved the function of an existing BioBrick Part or Device. First, we re-characterize the LacO-RFP BioBrick BBa_J04450 (Biology Results Section) and improve it by integrating it with an aceB-LacI iron sensor (Biobrick BBa_K1163102). Together, these Biobricks create a Fur Inverter (see Biology Results Section) that activates gene expression in response to iron.
- We collaborated with other iGEM teams: we participated to Purdue and UGent online survey and had a nice workshop with Paris-Saclay team. We are also setting up a scientific collaboration with the Edimburgh iGEM team that is working on and environmental sensor using the Fur System.
- As our project involves treating a human genetic disorder using genetically-engineered bacteria, it has numerous implications for human health, safety and security, and ethics. To enable safe delivery of therapeutic bacteria to the human intestine, we designed and constructed a polymer-based capsule. We also explored safety and ethics consequences of our project as the Human Practices studies:
- We discussed the medical feasibility and implications of this treatment in a series of interviews and discussions with renowned experts on iron overload diseases.
- We carefully analyzed the safety issues and potential consequences of our bacteria using consequence-based decision tree.
- We organized and hosted a scientific conference on iron overload disorders and perceptions of our our project on 18 September 2013. This conference was attended by both medical experts and iron disorder patients.
- We prepared and disseminated a patient perception survey about how our treatment could potentially help hemochromatosis patients. We received and analyzed the results from over 250 patients who responded to our survey.
- We wrote an extensive report on our conclusions about synthetic biology , which is the fruit of our many discussions over the summer.
Silver Medal (requires each of the four following goals)
- We experimentally validated that the aceB-sfGFP biobrick acts as an iron-reponsive biosensor (see Biology Results Section).
- We document the characterization of our aceB-sfGFP and other BioBricks in "Main Page" section of the Parts Registry.
- We submitted the following BioBricks parts to the iGEM Parts Registry. The 4 sfGFP constructs function as iron sensors and the lacI constructs are used to build a Fur-based genetic inverter that activates gene expression in response to iron. These 8 Biobricks have been received and accepted by the Registry of Standard Biological Parts.
- BBa_K1163102: AceB-sfGFP
- BBa_K1163103: AceB-lacI
- BBa_K1163105: FepA-sfGFP
- BBa_K1163106: FepA-lacI
- BBa_K1163108: Fes-sfGFP
- BBa_K1163109: Fes-lacI
- BBa_K1163111: YncE-sfGFP
- BBa_K1163112: YncE-LacI
- We describe how our project has implications for safety and ethics
Bronze Medal (requires each of the five following goals)
- We registered our team for iGEM 2013 and are attending the European Regional Jamboree.
- We completed and submitted the Judging form.
- We made a comprehensive and accessible iGEM Evry 2013 Wiki to convey our educational and scientific advances.
- We will present a poster and give an oral presentation at the iGEM European Jamboree in Lyon, France.
- We document 22 BioBricks used in our project and submit 8 news BioBricks to the Parts Registry (see 8 submitted Biobricks in Silver Medal Requirements).