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Team:Paris Saclay/Protocols
From 2013.igem.org
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[[Team:Paris_Saclay/Parts|Biobricks]] | [[Team:Paris_Saclay/Parts|Biobricks]] | ||
[[Team:Paris_Saclay/Protocols|Protocols]] | [[Team:Paris_Saclay/Protocols|Protocols]] | ||
| + | [[Team:Paris_Saclay/Primers|Primers]] | ||
[[Team:Paris_Saclay/Safety|Safety]] | [[Team:Paris_Saclay/Safety|Safety]] | ||
{{Team:Paris_Saclay/incl_fin_menu_navigation}} | {{Team:Paris_Saclay/incl_fin_menu_navigation}} | ||
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='''Protocols'''= | ='''Protocols'''= | ||
| - | [[Team:Paris_Saclay/preparation|Preparation of | + | [[Team:Paris_Saclay/preparation|Preparation of supercompetent ''E.coli'' cells ]] |
| - | [[Team:Paris_Saclay/Protocols/Transformation|Transformation of | + | [[Team:Paris_Saclay/Protocols/Transformation|Transformation of supercompetent ''E.coli'' cells ]] |
| - | [[Team:Paris_Saclay/extraction|Extraction of the | + | [[Team:Paris_Saclay/extraction|Extraction of the bacterial genomic DNA by using NucleoSpin® Tissue ]] |
| - | [[Team:Paris_Saclay/mini_maxi|Plasmidic DNA Mini and Maxi | + | [[Team:Paris_Saclay/mini_maxi|Plasmidic DNA Mini and Maxi Preparation ]] |
[[Team:Paris_Saclay/protocols/pcr|PCR for the bacterial genomic DNA ]] | [[Team:Paris_Saclay/protocols/pcr|PCR for the bacterial genomic DNA ]] | ||
| - | [[Team:Paris_Saclay/electro| | + | [[Team:Paris_Saclay/electro|Electroelution ]] |
| - | [[Team:Paris_Saclay/gibson|Gibson Assembly | + | [[Team:Paris_Saclay/gibson|Gibson Assembly Method ]] |
[[Team:Paris_Saclay/gel|Gel Electrophoresis ]] | [[Team:Paris_Saclay/gel|Gel Electrophoresis ]] | ||
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[[Team:Paris_Saclay/digestion_ligation|Digestion and Ligation ]] | [[Team:Paris_Saclay/digestion_ligation|Digestion and Ligation ]] | ||
| - | [http://www.mn-net.com/tabid/1452/default.aspx Gel | + | [http://www.mn-net.com/tabid/1452/default.aspx Gel Purification ] |
[[Team:Paris_Saclay/transduction|Transduction ]] | [[Team:Paris_Saclay/transduction|Transduction ]] | ||
Latest revision as of 20:40, 3 October 2013
Protocols
Preparation of supercompetent E.coli cells
Transformation of supercompetent E.coli cells
Extraction of the bacterial genomic DNA by using NucleoSpin® Tissue
Plasmidic DNA Mini and Maxi Preparation
PCR for the bacterial genomic DNA
[http://www.mn-net.com/tabid/1452/default.aspx Gel Purification ]
Protocols written by Sheng (the 4 first), Nadia (Transduction) and Abdou
