Team:Paris Saclay/Primers

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{{Team:Paris_Saclay/incl_debut}}
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{{Team:Paris_Saclay/incl_menu_navigation}}
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Labwork
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[[Team:Paris_Saclay/Labwork|Overview]]
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[[Team:Paris_Saclay/Parts|Biobricks]]
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[[Team:Paris_Saclay/Protocols|Protocols]]
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[[Team:Paris_Saclay/Primers|Primers]]
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[[Team:Paris_Saclay/Safety|Safety]]
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{{Team:Paris_Saclay/incl_fin_menu_navigation}}
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{{Team:Paris_Saclay/incl_contenu}}
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='''Primers'''=
{| class="wikitable"
{| class="wikitable"
|-
|-
 +
! scope="col"| Number
! scope="col"| Name
! scope="col"| Name
-
! scope="col"| Project
+
! scope="col"| Sequence 5' to 3'
-
! scope="col"| Sequence
+
! scope="col"| Organism
-
! scope="col"| Description
+
|-
|-
-
! scope="row"| BBa_C0051-Forward
+
! scope="row"| iPS33
-
| Gemote
+
| pfnr_up
-
| atgagcacaaaaaagaaaccatt
+
| ATATGAATTCGCGGCCGCTTCTAGAGT
-
| No description
+
| E.coli
|-
|-
-
! scope="row"| BBa_C0051-Forward
+
! scope="row"| iPS34
-
| Gemote
+
| pfnr_down
-
| atgagcacaaaaaagaaaccatt
+
| ATATCTGCAGCGGCCGCTACTAG
-
| No description
+
TAGTTCTTCGCTATTGAGAATAACATG
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| E.coli
|-
|-
-
! scope="row"| [http://www.instructables.com/id/Arduino-PCR-thermal-cycler-for-under-85/ Arduino PCR]
+
! scope="row"| iPS35
-
| $85
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| bphR2_up
-
| No heated lid, room for only 2 tubes, fan-only cooling.
+
| ATATGAATTCGCGGCCGCTTCTAGA
 +
TGGAACTGCATGACCTGGA
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| P. pseuoalcaligenes
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|-
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! scope="row"| iPS36
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| bphR2_down
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| ATATCTGCAGCGGCCGCTACTAGTAT
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CAATCCGAAAACAGCTCGA
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| P. pseuoalcaligenes
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|-
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! scope="row"| iPS37
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| bphR1_up
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| ATATGAATTCGCGGCCGCTTCTAGAG
 +
ATGTCCCTGGGTGACATGCGTG
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| P. pseuoalcaligenes
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|-
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! scope="row"| iPS38
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| bphR1_down
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| ATATCTGCAGCGGCCGCTACTAGTAA
 +
GCAGTCAATCGGCGGTAGCGC
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| P. pseuoalcaligenes
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|-
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! scope="row"| iPS39
 +
| bphA1_up
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| ATATGAATTCGCGGCCGCTTCTAGAGC
 +
GCCAACATCCCACGTGTTTG
 +
| P. pseuoalcaligenes
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|-
 +
! scope="row"| iPS40
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| bphA1_down
 +
| ATATCTGCAGCGGCCGCTACTAGTACC
 +
CCCGGATCGCCTCCGGCGTC
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| P. pseuoalcaligenes
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|-
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! scope="row"| iPS41
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| SB-prep-3P-1
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| GCCGCTGCAGTCCGGCAAAAAA
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| pSB1C3, E.coli
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|-
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! scope="row"| iPS42
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| SB-prep-2Ea
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| TGCCACCTGACGTCTAAGAA
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| pSB1C3, E.coli
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|-
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! scope="row"| iPS43
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| VF2
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| TGCCACCTGACGTCTAAGAA
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| pSB1C3, E.coli
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|-
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! scope="row"| iPS44
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| VR
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| ATTACCGCCTTTGAGTGAGC
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| pSB1C3, E.coli
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|-
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! scope="row"| iPS45
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| Prefix-prom_nirB 
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| TCATGAATTCGCGGCCGCTTCTAGA
 +
GCTACGTGCATCTATTTCTAT
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| E.coli
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|-
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! scope="row"| iPS46
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| suffixe-prom_nirB
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| TACTCTGCAGCGGCCGCTACTAGTA
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TGCCTCGATTTCTTTTCTAT
 +
| E.coli
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|-
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! scope="row"| iPS47
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| Prefix-prom_narK
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| TCATGAATTCGCGGCCGCTTCTAGAG
 +
ACATCGGTAAGGGTAGGGAT
 +
| E.coli
 +
|-
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! scope="row"| iPS48
 +
| suffixe-prom_narK
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| TACTCTGCAGCGGCCGCTACTAGTAA
 +
GACACCTCTGATACTCGTT
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| E.coli
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|-
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! scope="row"| iPS49
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| prefix-prom_narG
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| TCATGAATTCGCGGCCGCTTCTAGAG
 +
GGAATTTACTTTATTTTTCA
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| E.coli
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|-
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! scope="row"| iPS50
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| suffixe-prom_narG
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| TACTCTGCAGCGGCCGCTACTAGTAT
 +
TCTCCTGTGGGAGCCTGTC
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| E.coli
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|-
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! scope="row"| iPS51
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| Prefix from pSB1C3
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| CTCTAGAAGCGGCCGCGAATTC
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| pSB1C3, E.coli
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|-
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! scope="row"| iPS52
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| sufix from pSB1C3
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| GTACCTGCAGCGGCCGCTACTAGTA
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| pSB1C3,E.coli
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|-
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! scope="row"| iPS53
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| prefix-RBS-AmilCP
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| CATAGAATTCGCGGCCGCTTCTAGAGAAAGAGG
 +
AGAAATACTAGATGAGTGTGATCGCTAAACAAAT
 +
| pSB1C3,E.coli
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|-
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! scope="row"| iPS54
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| Bba_BphR2-part1-Gibson-Forward
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| GAATTCGCGGCCGCTTCTAGAGATGGAA
 +
CTGCATGACCTGGAT
 +
| P. pseuoalcaligenes
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|-
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! scope="row"| iPS55
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| Bba_BphR2-part1-Gibson-Reverse
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| CAGTTCGTCCTGTAGTGCGG
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| P. pseuoalcaligenes
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|-
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! scope="row"| iPS56
 +
| Bba_BphR2-part2-Gibson-Forward
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| CACCGCACTACAGGACGAAC
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| P. pseuoalcaligenes
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|-
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! scope="row"| iPS57
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| Bba_BphR2-part2-Gibson-Reverse
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| CTGCAGCGGCCGCTACTAGTATCAATCCG
 +
AAAACAGCTCGAACA
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| P. pseuoalcaligenes
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|-
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! scope="row"| iPS58
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| Bba_RBS-BphR2-part1-Gibson-Forward
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| GAATTCGCGGGCCGCTTCTAGAGAAAGAGG
 +
AGAAATACTAGATGGAACTGCATGACCTGGAT
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| P. pseuoalcaligenes
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|-
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! scope="row"| iPS59
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| Bba_FNR-part1-Gibson-Forward
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| GAATTCGCGGCCGCTTCTAGAGATGATCCC
 +
GGAAAAGCGAAT
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| E.coli
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|-
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! scope="row"| iPS60
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| Bba_FNR-part1-Gibson-Reverse
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| CGGAACTCACGAGGGGAGAA
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| E.coli
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|-
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! scope="row"| iPS61
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| Bba_FNR-part2-Gibson-Forward
 +
| TTCTCCCCTCGTGAGTTCCG
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| E.coli
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|-
 +
! scope="row"| iPS62
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| Bba_FNR-part2-Gibson-Reverse
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| CTGCAGCGGCCGCTACTAGTATCAGGCAAC
 +
GTTACGCGTAT
 +
| E.coli
 +
|-
 +
! scope="row"| iPS63
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| Bba_RBS-FNR-part1-Gibson-Forward
 +
| GAATTCGCGGCCGCTTCTAGAGAAAGAGGA
 +
GAAATACTAGATGATCCCGGAAAAGCGAATT
 +
| E.coli
 +
|-
 +
! scope="row"| iPS64
 +
| Suffix-from-pSB1C3-Gibson
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| TACTAGTAGCGGCCGCTGCAG
 +
| pSB1C3,E.coli
 +
|-
 +
! scope="row"| iPS65
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| Prefix-from-pSB1C3-Gibson
 +
| CTCTAGAAGCGGCCGCGAATTC
 +
| pSB1C3,E.coli
|-
|-
|}
|}
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{{Team:Paris_Saclay/incl_fin}}

Latest revision as of 00:23, 5 October 2013

Labwork Overview Biobricks Protocols Primers Safety

Primers

Number Name Sequence 5' to 3' Organism
iPS33 pfnr_up ATATGAATTCGCGGCCGCTTCTAGAGT E.coli
iPS34 pfnr_down ATATCTGCAGCGGCCGCTACTAG

TAGTTCTTCGCTATTGAGAATAACATG

E.coli
iPS35 bphR2_up ATATGAATTCGCGGCCGCTTCTAGA

TGGAACTGCATGACCTGGA

P. pseuoalcaligenes
iPS36 bphR2_down ATATCTGCAGCGGCCGCTACTAGTAT

CAATCCGAAAACAGCTCGA

P. pseuoalcaligenes
iPS37 bphR1_up ATATGAATTCGCGGCCGCTTCTAGAG

ATGTCCCTGGGTGACATGCGTG

P. pseuoalcaligenes
iPS38 bphR1_down ATATCTGCAGCGGCCGCTACTAGTAA

GCAGTCAATCGGCGGTAGCGC

P. pseuoalcaligenes
iPS39 bphA1_up ATATGAATTCGCGGCCGCTTCTAGAGC

GCCAACATCCCACGTGTTTG

P. pseuoalcaligenes
iPS40 bphA1_down ATATCTGCAGCGGCCGCTACTAGTACC

CCCGGATCGCCTCCGGCGTC

P. pseuoalcaligenes
iPS41 SB-prep-3P-1 GCCGCTGCAGTCCGGCAAAAAA pSB1C3, E.coli
iPS42 SB-prep-2Ea TGCCACCTGACGTCTAAGAA pSB1C3, E.coli
iPS43 VF2 TGCCACCTGACGTCTAAGAA pSB1C3, E.coli
iPS44 VR ATTACCGCCTTTGAGTGAGC pSB1C3, E.coli
iPS45 Prefix-prom_nirB TCATGAATTCGCGGCCGCTTCTAGA

GCTACGTGCATCTATTTCTAT

E.coli
iPS46 suffixe-prom_nirB TACTCTGCAGCGGCCGCTACTAGTA

TGCCTCGATTTCTTTTCTAT

E.coli
iPS47 Prefix-prom_narK TCATGAATTCGCGGCCGCTTCTAGAG

ACATCGGTAAGGGTAGGGAT

E.coli
iPS48 suffixe-prom_narK TACTCTGCAGCGGCCGCTACTAGTAA

GACACCTCTGATACTCGTT

E.coli
iPS49 prefix-prom_narG TCATGAATTCGCGGCCGCTTCTAGAG

GGAATTTACTTTATTTTTCA

E.coli
iPS50 suffixe-prom_narG TACTCTGCAGCGGCCGCTACTAGTAT

TCTCCTGTGGGAGCCTGTC

E.coli
iPS51 Prefix from pSB1C3 CTCTAGAAGCGGCCGCGAATTC pSB1C3, E.coli
iPS52 sufix from pSB1C3 GTACCTGCAGCGGCCGCTACTAGTA pSB1C3,E.coli
iPS53 prefix-RBS-AmilCP CATAGAATTCGCGGCCGCTTCTAGAGAAAGAGG

AGAAATACTAGATGAGTGTGATCGCTAAACAAAT

pSB1C3,E.coli
iPS54 Bba_BphR2-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGATGGAA

CTGCATGACCTGGAT

P. pseuoalcaligenes
iPS55 Bba_BphR2-part1-Gibson-Reverse CAGTTCGTCCTGTAGTGCGG P. pseuoalcaligenes
iPS56 Bba_BphR2-part2-Gibson-Forward CACCGCACTACAGGACGAAC P. pseuoalcaligenes
iPS57 Bba_BphR2-part2-Gibson-Reverse CTGCAGCGGCCGCTACTAGTATCAATCCG

AAAACAGCTCGAACA

P. pseuoalcaligenes
iPS58 Bba_RBS-BphR2-part1-Gibson-Forward GAATTCGCGGGCCGCTTCTAGAGAAAGAGG

AGAAATACTAGATGGAACTGCATGACCTGGAT

P. pseuoalcaligenes
iPS59 Bba_FNR-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGATGATCCC

GGAAAAGCGAAT

E.coli
iPS60 Bba_FNR-part1-Gibson-Reverse CGGAACTCACGAGGGGAGAA E.coli
iPS61 Bba_FNR-part2-Gibson-Forward TTCTCCCCTCGTGAGTTCCG E.coli
iPS62 Bba_FNR-part2-Gibson-Reverse CTGCAGCGGCCGCTACTAGTATCAGGCAAC

GTTACGCGTAT

E.coli
iPS63 Bba_RBS-FNR-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGAAAGAGGA

GAAATACTAGATGATCCCGGAAAAGCGAATT

E.coli
iPS64 Suffix-from-pSB1C3-Gibson TACTAGTAGCGGCCGCTGCAG pSB1C3,E.coli
iPS65 Prefix-from-pSB1C3-Gibson CTCTAGAAGCGGCCGCGAATTC pSB1C3,E.coli

Retrieved from "http://2013.igem.org/Team:Paris_Saclay/Primers"