Team:TU-Delft/Notebook/2013/09/29/

From 2013.igem.org

(Difference between revisions)

Latest revision as of 09:17, 4 October 2013

Notebook

29th of September

Lab work

1. Membrane experiment is carried out to quantify the AIPs diffusion across the membrane for the band-aid model.
2. Sequence coverage from Tandem MS for Signiferin Peptide :

@@ Line 8: / Line 8: @@
 <html>
 </br></br></br></br></br></br>
+<a name="lab29"></a>
 </html>
 <br><br><br><br>
-== 28th of September==
+== 29th of September==
 <br>
@@ Line 22: / Line 24: @@
 . Membrane experiment is carried out to quantify the AIPs diffusion across the membrane for the band-aid model.<br>
-. The corrected construct of the pBAD AIP Receiver GFP colonies 6, 7 and 8 were induced with Arabinose 0.1% and AIP 1 µM and 10 µM. Controls of No Arabinose and No AIP, No Arabinose and only AIP, No AIP and only arabinose induction were also done. <br>
+. Sequence coverage from Tandem MS for Signiferin Peptide : <br>
+<center>
-Hours after AIP induction the samples were analyzed on FACS for signals of GFP. <br>
+<img src="https://static.igem.org/mediawiki/2013/0/0c/Sumo4.png">
+</center>
-. pBAD Ulp TT pT7 SUMO Signiferin construct was induced with Arabinose and IPTG to check for the cleavage of SUMO-Peptide by Ulp protease. The cells were french pressed and stored in -80 degree. MIC experiment will be carried out on it and an SDS Page will be run to check for Ulp, SUMO-Peptide and cleaved peptide bands.<br>
-. Samples of the pT7 Sumo peptides were given for MS measurements. <br>

Sun	Mon	Tue	Wed	Thu	Fri	Sat
						1
2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30

Sun	Mon	Tue	Wed	Thu	Fri	Sat
	1	2	3	4	5	6
7	8	9	10	11	12	13
14	15	16	17	18	19	20
21	22	23	24	25	26	27
28	29	30	31

Sun	Mon	Tue	Wed	Thu	Fri	Sat
				1	2	3
4	5	6	7	8	9	10
11	12	13	14	15	16	17
18	19	20	21	22	23	24
25	26	27	28	29	30	31

Sun	Mon	Tue	Wed	Thu	Fri	Sat
1	2	3	4	5	6	7
8	9	10	11	12	13	14
15	16	17	18	19	20	21
22	23	24	25	26	27	28
29	30