Team:Paris Saclay/Primers

From 2013.igem.org

(Difference between revisions)
(→Primers)
 
(13 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Team:Paris_Saclay/incl_debut}}
 +
 +
{{Team:Paris_Saclay/incl_menu_navigation}}
 +
Labwork
 +
[[Team:Paris_Saclay/Labwork|Overview]]
 +
[[Team:Paris_Saclay/Parts|Biobricks]]
 +
[[Team:Paris_Saclay/Protocols|Protocols]]
 +
[[Team:Paris_Saclay/Primers|Primers]]
 +
[[Team:Paris_Saclay/Safety|Safety]]
 +
{{Team:Paris_Saclay/incl_fin_menu_navigation}}
 +
 +
{{Team:Paris_Saclay/incl_contenu}}
 +
 +
='''Primers'''=
 +
{| class="wikitable"
{| class="wikitable"
|-
|-
! scope="col"| Number
! scope="col"| Number
! scope="col"| Name
! scope="col"| Name
-
! scope="col"| Sequence
+
! scope="col"| Sequence 5' to 3'
! scope="col"| Organism
! scope="col"| Organism
|-
|-
Line 13: Line 28:
! scope="row"| iPS34
! scope="row"| iPS34
| pfnr_down
| pfnr_down
-
| ATATCTGCAGCGGCCGCTACTAGTAGTTCTTCGCTATTGAGAATAACATG
+
| ATATCTGCAGCGGCCGCTACTAG
 +
TAGTTCTTCGCTATTGAGAATAACATG
| E.coli
| E.coli
|-
|-
! scope="row"| iPS35
! scope="row"| iPS35
| bphR2_up
| bphR2_up
-
| ATATGAATTCGCGGCCGCTTCTAGATGGAACTGCATGACCTGGA
+
| ATATGAATTCGCGGCCGCTTCTAGA
 +
TGGAACTGCATGACCTGGA
| P. pseuoalcaligenes
| P. pseuoalcaligenes
|-
|-
! scope="row"| iPS36
! scope="row"| iPS36
| bphR2_down
| bphR2_down
-
| ATATCTGCAGCGGCCGCTACTAGTATCAATCCGAAAACAGCTCGA
+
| ATATCTGCAGCGGCCGCTACTAGTAT
 +
CAATCCGAAAACAGCTCGA
| P. pseuoalcaligenes
| P. pseuoalcaligenes
|-
|-
! scope="row"| iPS37
! scope="row"| iPS37
| bphR1_up
| bphR1_up
-
| ATATGAATTCGCGGCCGCTTCTAGAG ATGTCCCTGGGTGACATGCGTG
+
| ATATGAATTCGCGGCCGCTTCTAGAG
 +
ATGTCCCTGGGTGACATGCGTG
| P. pseuoalcaligenes
| P. pseuoalcaligenes
|-
|-
-
! scope="row"|
+
! scope="row"| iPS38
-
|
+
| bphR1_down
-
|
+
| ATATCTGCAGCGGCCGCTACTAGTAA
-
|
+
GCAGTCAATCGGCGGTAGCGC
 +
| P. pseuoalcaligenes
|-
|-
-
! scope="row"|
+
! scope="row"| iPS39
-
|
+
| bphA1_up
-
|
+
| ATATGAATTCGCGGCCGCTTCTAGAGC
-
|
+
GCCAACATCCCACGTGTTTG
 +
| P. pseuoalcaligenes
|-
|-
-
! scope="row"|
+
! scope="row"| iPS40
-
|
+
| bphA1_down
-
|
+
| ATATCTGCAGCGGCCGCTACTAGTACC
-
|
+
CCCGGATCGCCTCCGGCGTC
 +
| P. pseuoalcaligenes
|-
|-
-
! scope="row"|
+
! scope="row"| iPS41
-
|
+
| SB-prep-3P-1
-
|
+
| GCCGCTGCAGTCCGGCAAAAAA
-
|
+
| pSB1C3, E.coli
 +
|-
 +
! scope="row"| iPS42
 +
| SB-prep-2Ea
 +
| TGCCACCTGACGTCTAAGAA
 +
| pSB1C3, E.coli
 +
|-
 +
! scope="row"| iPS43
 +
| VF2
 +
| TGCCACCTGACGTCTAAGAA
 +
| pSB1C3, E.coli
 +
|-
 +
! scope="row"| iPS44
 +
| VR
 +
| ATTACCGCCTTTGAGTGAGC
 +
| pSB1C3, E.coli
 +
|-
 +
! scope="row"| iPS45
 +
| Prefix-prom_nirB 
 +
| TCATGAATTCGCGGCCGCTTCTAGA
 +
GCTACGTGCATCTATTTCTAT
 +
| E.coli
 +
|-
 +
! scope="row"| iPS46
 +
| suffixe-prom_nirB
 +
| TACTCTGCAGCGGCCGCTACTAGTA
 +
TGCCTCGATTTCTTTTCTAT
 +
| E.coli
 +
|-
 +
! scope="row"| iPS47
 +
| Prefix-prom_narK
 +
| TCATGAATTCGCGGCCGCTTCTAGAG
 +
ACATCGGTAAGGGTAGGGAT
 +
| E.coli
 +
|-
 +
! scope="row"| iPS48
 +
| suffixe-prom_narK
 +
| TACTCTGCAGCGGCCGCTACTAGTAA
 +
GACACCTCTGATACTCGTT
 +
| E.coli
 +
|-
 +
! scope="row"| iPS49
 +
| prefix-prom_narG
 +
| TCATGAATTCGCGGCCGCTTCTAGAG
 +
GGAATTTACTTTATTTTTCA
 +
| E.coli
 +
|-
 +
! scope="row"| iPS50
 +
| suffixe-prom_narG
 +
| TACTCTGCAGCGGCCGCTACTAGTAT
 +
TCTCCTGTGGGAGCCTGTC
 +
| E.coli
 +
|-
 +
! scope="row"| iPS51
 +
| Prefix from pSB1C3
 +
| CTCTAGAAGCGGCCGCGAATTC
 +
| pSB1C3, E.coli
 +
|-
 +
! scope="row"| iPS52
 +
| sufix from pSB1C3
 +
| GTACCTGCAGCGGCCGCTACTAGTA
 +
| pSB1C3,E.coli
 +
|-
 +
! scope="row"| iPS53
 +
| prefix-RBS-AmilCP
 +
| CATAGAATTCGCGGCCGCTTCTAGAGAAAGAGG
 +
AGAAATACTAGATGAGTGTGATCGCTAAACAAAT
 +
| pSB1C3,E.coli
 +
|-
 +
! scope="row"| iPS54
 +
| Bba_BphR2-part1-Gibson-Forward
 +
| GAATTCGCGGCCGCTTCTAGAGATGGAA
 +
CTGCATGACCTGGAT
 +
| P. pseuoalcaligenes
 +
|-
 +
! scope="row"| iPS55
 +
| Bba_BphR2-part1-Gibson-Reverse
 +
| CAGTTCGTCCTGTAGTGCGG
 +
| P. pseuoalcaligenes
 +
|-
 +
! scope="row"| iPS56
 +
| Bba_BphR2-part2-Gibson-Forward
 +
| CACCGCACTACAGGACGAAC
 +
| P. pseuoalcaligenes
 +
|-
 +
! scope="row"| iPS57
 +
| Bba_BphR2-part2-Gibson-Reverse
 +
| CTGCAGCGGCCGCTACTAGTATCAATCCG
 +
AAAACAGCTCGAACA
 +
| P. pseuoalcaligenes
 +
|-
 +
! scope="row"| iPS58
 +
| Bba_RBS-BphR2-part1-Gibson-Forward
 +
| GAATTCGCGGGCCGCTTCTAGAGAAAGAGG
 +
AGAAATACTAGATGGAACTGCATGACCTGGAT
 +
| P. pseuoalcaligenes
 +
|-
 +
! scope="row"| iPS59
 +
| Bba_FNR-part1-Gibson-Forward
 +
| GAATTCGCGGCCGCTTCTAGAGATGATCCC
 +
GGAAAAGCGAAT
 +
| E.coli
 +
|-
 +
! scope="row"| iPS60
 +
| Bba_FNR-part1-Gibson-Reverse
 +
| CGGAACTCACGAGGGGAGAA
 +
| E.coli
 +
|-
 +
! scope="row"| iPS61
 +
| Bba_FNR-part2-Gibson-Forward
 +
| TTCTCCCCTCGTGAGTTCCG
 +
| E.coli
 +
|-
 +
! scope="row"| iPS62
 +
| Bba_FNR-part2-Gibson-Reverse
 +
| CTGCAGCGGCCGCTACTAGTATCAGGCAAC
 +
GTTACGCGTAT
 +
| E.coli
 +
|-
 +
! scope="row"| iPS63
 +
| Bba_RBS-FNR-part1-Gibson-Forward
 +
| GAATTCGCGGCCGCTTCTAGAGAAAGAGGA
 +
GAAATACTAGATGATCCCGGAAAAGCGAATT
 +
| E.coli
 +
|-
 +
! scope="row"| iPS64
 +
| Suffix-from-pSB1C3-Gibson
 +
| TACTAGTAGCGGCCGCTGCAG
 +
| pSB1C3,E.coli
 +
|-
 +
! scope="row"| iPS65
 +
| Prefix-from-pSB1C3-Gibson
 +
| CTCTAGAAGCGGCCGCGAATTC
 +
| pSB1C3,E.coli
|-
|-
-
 
|}
|}
 +
 +
{{Team:Paris_Saclay/incl_fin}}

Latest revision as of 00:23, 5 October 2013

Labwork Overview Biobricks Protocols Primers Safety

Primers

Number Name Sequence 5' to 3' Organism
iPS33 pfnr_up ATATGAATTCGCGGCCGCTTCTAGAGT E.coli
iPS34 pfnr_down ATATCTGCAGCGGCCGCTACTAG

TAGTTCTTCGCTATTGAGAATAACATG

E.coli
iPS35 bphR2_up ATATGAATTCGCGGCCGCTTCTAGA

TGGAACTGCATGACCTGGA

P. pseuoalcaligenes
iPS36 bphR2_down ATATCTGCAGCGGCCGCTACTAGTAT

CAATCCGAAAACAGCTCGA

P. pseuoalcaligenes
iPS37 bphR1_up ATATGAATTCGCGGCCGCTTCTAGAG

ATGTCCCTGGGTGACATGCGTG

P. pseuoalcaligenes
iPS38 bphR1_down ATATCTGCAGCGGCCGCTACTAGTAA

GCAGTCAATCGGCGGTAGCGC

P. pseuoalcaligenes
iPS39 bphA1_up ATATGAATTCGCGGCCGCTTCTAGAGC

GCCAACATCCCACGTGTTTG

P. pseuoalcaligenes
iPS40 bphA1_down ATATCTGCAGCGGCCGCTACTAGTACC

CCCGGATCGCCTCCGGCGTC

P. pseuoalcaligenes
iPS41 SB-prep-3P-1 GCCGCTGCAGTCCGGCAAAAAA pSB1C3, E.coli
iPS42 SB-prep-2Ea TGCCACCTGACGTCTAAGAA pSB1C3, E.coli
iPS43 VF2 TGCCACCTGACGTCTAAGAA pSB1C3, E.coli
iPS44 VR ATTACCGCCTTTGAGTGAGC pSB1C3, E.coli
iPS45 Prefix-prom_nirB TCATGAATTCGCGGCCGCTTCTAGA

GCTACGTGCATCTATTTCTAT

E.coli
iPS46 suffixe-prom_nirB TACTCTGCAGCGGCCGCTACTAGTA

TGCCTCGATTTCTTTTCTAT

E.coli
iPS47 Prefix-prom_narK TCATGAATTCGCGGCCGCTTCTAGAG

ACATCGGTAAGGGTAGGGAT

E.coli
iPS48 suffixe-prom_narK TACTCTGCAGCGGCCGCTACTAGTAA

GACACCTCTGATACTCGTT

E.coli
iPS49 prefix-prom_narG TCATGAATTCGCGGCCGCTTCTAGAG

GGAATTTACTTTATTTTTCA

E.coli
iPS50 suffixe-prom_narG TACTCTGCAGCGGCCGCTACTAGTAT

TCTCCTGTGGGAGCCTGTC

E.coli
iPS51 Prefix from pSB1C3 CTCTAGAAGCGGCCGCGAATTC pSB1C3, E.coli
iPS52 sufix from pSB1C3 GTACCTGCAGCGGCCGCTACTAGTA pSB1C3,E.coli
iPS53 prefix-RBS-AmilCP CATAGAATTCGCGGCCGCTTCTAGAGAAAGAGG

AGAAATACTAGATGAGTGTGATCGCTAAACAAAT

pSB1C3,E.coli
iPS54 Bba_BphR2-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGATGGAA

CTGCATGACCTGGAT

P. pseuoalcaligenes
iPS55 Bba_BphR2-part1-Gibson-Reverse CAGTTCGTCCTGTAGTGCGG P. pseuoalcaligenes
iPS56 Bba_BphR2-part2-Gibson-Forward CACCGCACTACAGGACGAAC P. pseuoalcaligenes
iPS57 Bba_BphR2-part2-Gibson-Reverse CTGCAGCGGCCGCTACTAGTATCAATCCG

AAAACAGCTCGAACA

P. pseuoalcaligenes
iPS58 Bba_RBS-BphR2-part1-Gibson-Forward GAATTCGCGGGCCGCTTCTAGAGAAAGAGG

AGAAATACTAGATGGAACTGCATGACCTGGAT

P. pseuoalcaligenes
iPS59 Bba_FNR-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGATGATCCC

GGAAAAGCGAAT

E.coli
iPS60 Bba_FNR-part1-Gibson-Reverse CGGAACTCACGAGGGGAGAA E.coli
iPS61 Bba_FNR-part2-Gibson-Forward TTCTCCCCTCGTGAGTTCCG E.coli
iPS62 Bba_FNR-part2-Gibson-Reverse CTGCAGCGGCCGCTACTAGTATCAGGCAAC

GTTACGCGTAT

E.coli
iPS63 Bba_RBS-FNR-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGAAAGAGGA

GAAATACTAGATGATCCCGGAAAAGCGAATT

E.coli
iPS64 Suffix-from-pSB1C3-Gibson TACTAGTAGCGGCCGCTGCAG pSB1C3,E.coli
iPS65 Prefix-from-pSB1C3-Gibson CTCTAGAAGCGGCCGCGAATTC pSB1C3,E.coli

Retrieved from "http://2013.igem.org/Team:Paris_Saclay/Primers"