Team:TMU-Tokyo/Parts

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  <h1>Submitted parts</h1>
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<center><groupparts>iGEM013 TMU-Tokyo</groupparts></center>
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!align="center"|[[Team:TMU-Tokyo|Home]]
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!align="center"|[[Team:TMU-Tokyo/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=TMU-Tokyo Official Team Profile]
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!align="center"|[[Team:TMU-Tokyo/Project|Project]]
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!align="center"|[[Team:TMU-Tokyo/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:TMU-Tokyo/Modeling|Modeling]]
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!align="center"|[[Team:TMU-Tokyo/Notebook|Notebook]]
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!align="center"|[[Team:TMU-Tokyo/Safety|Safety]]
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!align="center"|[[Team:TMU-Tokyo/Attributions|Attributions]]
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<h1>Parts data</h1>
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<li><a href="http://parts.igem.org/Part:BBa_K1015013">BBa_K1015013</a>：<b>Km-Blunt end </b><br>
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  This is what connected the blunt end to cohesive end of pSB1C3.</li>
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<li><a href="http://parts.igem.org/Part:BBa_K1015014">BBa_K1015014</a>：<b>IRR/IRL(Ptet)-FLP-Sm(insert between lacA and cynR)</b><br>
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    This device produce FLP and AadA(streptomycin resistant gene) . But if site specific recombination be caused between IRR site and IRL site by FimE recombinase, pTet promoter change "OFF" state and FLP production stop.
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<li><a href="http://parts.igem.org/Part:BBa_K1015015">BBa_K1015015</a>：<b>araC-pBAD-hbiF-Tc(genome insertion parts between araC and araA)</b><br>
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    With bacteriophage λ recombination system, this device insert into Escherichia coli genome. This device produce hbiF recombinase and tetracycline resistance protein (tetA). HbiF production level are regulated by arabinose inducible promoter pBAD.</li>
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<li><a href="http://parts.igem.org/Part:BBa_K1015016">BBa_K1015016</a>：<b>lacI+FRT+Cm</b><br>
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  This is genome insertion part. The device is: R(SaApR)homology sequence(BBa_K1015018) + lacI(BBa_K1015008) + FRT(BBa_K1015009) + mhpA homology sequence(BBa_K1015009).
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<li><a href="http://parts.igem.org/Part:BBa_K1015017">BBa_K1015017</a>：<b>FRT+Ap(for RED system)</b><br>
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    This is genome insertion parts. Site      specific recombination site. if it happens by Flp sequences between 2 place of FRT is left out.  </li>
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
 
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Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for  users who wish to know more.
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<groupparts>iGEM013 TMU-Tokyo</groupparts>
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Latest revision as of 02:50, 28 September 2013



Submitted parts



<groupparts>iGEM013 TMU-Tokyo</groupparts>



Parts data
  1. BBa_K1015013：Km-Blunt end
    This is what connected the blunt end to cohesive end of pSB1C3.

    2.
  2. BBa_K1015014：IRR/IRL(Ptet)-FLP-Sm(insert between lacA and cynR)
    This device produce FLP and AadA(streptomycin resistant gene) . But if site specific recombination be caused between IRR site and IRL site by FimE recombinase, pTet promoter change "OFF" state and FLP production stop.

  3. BBa_K1015015：araC-pBAD-hbiF-Tc(genome insertion parts between araC and araA)
    With bacteriophage λ recombination system, this device insert into Escherichia coli genome. This device produce hbiF recombinase and tetracycline resistance protein (tetA). HbiF production level are regulated by arabinose inducible promoter pBAD.

    4.
  4. BBa_K1015016：lacI+FRT+Cm
    This is genome insertion part. The device is: R(SaApR)homology sequence(BBa_K1015018) + lacI(BBa_K1015008) + FRT(BBa_K1015009) + mhpA homology sequence(BBa_K1015009).

  5. BBa_K1015017：FRT+Ap(for RED system)
    This is genome insertion parts. Site specific recombination site. if it happens by Flp sequences between 2 place of FRT is left out.


    6. Retrieved from "http://2013.igem.org/Team:TMU-Tokyo/Parts"