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Team:CU-Boulder/Notebook/Protocols
From 2013.igem.org
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<dd>This protocol details how to prepare chemically competent cells.</dd> | <dd>This protocol details how to prepare chemically competent cells.</dd> | ||
</dl> | </dl> | ||
| + | <h4>Related Experiments</h4> | ||
| + | <ul> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_1">Experiment 2.1 - Competent Cell Efficiency</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_5_1">Experiment 5.1 - Calculating Competent Cell Efficiency</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_6_1">Experiment 6.1 - Competent Cell Efficiency</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_12_1">Experiment 12.1 - Calculating Competent Cell Efficiency</a></li> | ||
| + | </ul> | ||
</div> | </div> | ||
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<dd>In this protocol we cover how to use our chemically competent cells to perform transformations.</dd> | <dd>In this protocol we cover how to use our chemically competent cells to perform transformations.</dd> | ||
</dl> | </dl> | ||
| + | <h4>Related Experiments</h4> | ||
| + | <ul> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_1_1">Experiment 1.1 - Chemical Transformation Efficiency</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_2">Experiment 2.2 - Transformation Recovery Period</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_3">Experiment 2.3 - Transformation Recovery Medium</a></li> | ||
| + | </ul> | ||
</div> | </div> | ||
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<dd>Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.</dd> | <dd>Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.</dd> | ||
</dl> | </dl> | ||
| + | <h4>Related Experiments</h4> | ||
| + | <ul> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_3_1">Experiment 3.1 - Promega Miniprep Protocol</a></li> | ||
| + | </ul> | ||
</div> | </div> | ||
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/RestrictionDigests">Restriction (Double) Digests</a> <a href="https://static.igem.org/mediawiki/2013/f/f7/Restriction_Digests.pdf"><i class="icon-download"></i></a></dt> | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/RestrictionDigests">Restriction (Double) Digests</a> <a href="https://static.igem.org/mediawiki/2013/f/f7/Restriction_Digests.pdf"><i class="icon-download"></i></a></dt> | ||
<dd>Our method of performing restriction double digests is detailed in this protocol.</dd> | <dd>Our method of performing restriction double digests is detailed in this protocol.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/DNAPurification">Gel Purification of DNA</a> <a href="https://static.igem.org/mediawiki/2013/1/19/Gel_Purification_of_DNA.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>Here we detail a fast and simple method of purifying DNA from a gel.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/LigationReactions">Ligation Reactions</a> <a href="https://static.igem.org/mediawiki/2013/f/f2/Ligation_Reactions.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>This protocol covers how we set up out ligation reactions.</dd> | ||
</dl> | </dl> | ||
</div> | </div> | ||
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<dd>The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.</dd> | <dd>The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.</dd> | ||
</dl> | </dl> | ||
| + | <h4>Related Experiments</h4> | ||
| + | <ul> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_10_1">Experiment 10.1 - Preliminary Homemade Miniprep Buffer Experiment</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_2">Experiment 11.2 - Homemade vs. Qiagen Miniprep Buffers</a></li> | ||
| + | </ul> | ||
</div> | </div> | ||
| Line 56: | Line 92: | ||
<dd>This protocol covers how to recycle mini-columns used in plasmid miniprep systems.</dd> | <dd>This protocol covers how to recycle mini-columns used in plasmid miniprep systems.</dd> | ||
</dl> | </dl> | ||
| + | <h4>Related Experiments</h4> | ||
| + | <ul> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_1">Experiment 11.1 - Mini-column Recycling Protocol</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_3">Experiment 11.3 - Mini-column Recycling Protocol Pt. II</a></li> | ||
</div> | </div> | ||
| - | </ | + | <div class="shadow-bottom"> |
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ProteinMiniprep">Protein Miniprep</a> <a href="https://static.igem.org/mediawiki/2013/a/a7/Protein_Miniprep.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>This protocol covers how to extract proteins from cells.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| - | + | <div class="shadow-bottom"> | |
| - | + | <dl> | |
| - | + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ProteinPurificationAgaroseGel">Protein Purification Via Agarose Gel</a> <a href="https://static.igem.org/mediawiki/2013/6/61/ProteinPurificationAgaroseGel.pdf"><i class="icon-download"></i></a></dt> | |
| + | <dd>This protocol covers how to extract proteins from cells and purify it.</dd> | ||
| + | </dl> | ||
| + | <h4>Related Experiments</h4> | ||
| + | <ul> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_6_2">Experiment 6.2 – Gel Separation of Proteins Methods</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_14_1">Experiment 14.1 – Gel Separation of Proteins</a></li> | ||
| + | </div> | ||
| - | + | </html> | |
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Latest revision as of 00:57, 26 September 2013
- Embedding PDF Files
- In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.
- Embedding Google Docs
- Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.
- Chemically Competent Cells
- This protocol details how to prepare chemically competent cells.
Related Experiments
- Chemical Transformations
- In this protocol we cover how to use our chemically competent cells to perform transformations.
Related Experiments
- Plasmid Minipreps
- Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.
Related Experiments
- Restriction (Double) Digests
- Our method of performing restriction double digests is detailed in this protocol.
- Gel Purification of DNA
- Here we detail a fast and simple method of purifying DNA from a gel.
- Ligation Reactions
- This protocol covers how we set up out ligation reactions.
- Homemade Buffer Compositions
- The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.
Related Experiments
- Recycling DNA Mini-columns
- This protocol covers how to recycle mini-columns used in plasmid miniprep systems.
Related Experiments
- Protein Miniprep
- This protocol covers how to extract proteins from cells.
- Protein Purification Via Agarose Gel
- This protocol covers how to extract proteins from cells and purify it.
