Week1

From 2013.igem.org

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<html>
<html>
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<head>
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<head>
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<title>TzuChiU Formosa Notebook Week 1</title>
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  <head>
-
<meta http-equiv="X-UA-Compatible" content="IE=Edge"/><meta http-equiv="Content-Type" content="text/html; charset=utf-8"/><title>NodeFire Save Document</title>
+
  <title>TzuChiU Formosa</title>
 +
 
 +
      <!--[START-WEEK-HIDDEN MENU]-->
 +
<link rel="stylesheet" href="https://googledrive.com/host/0B6PQpiOVXRVgOUdSZzdta2FHek0" type="text/css" charset="utf-8"/>
 +
        <script type="text/javascript" src="https://googledrive.com/host/0B6PQpiOVXRVgVml5UDQ3REdXME0"></script>
 +
<!--[END-WEEK-HIDDEN MENU]-->
 +
 
 +
 
 +
<link rel="stylesheet" type="text/css" href="https://googledrive.com/host/0B6PQpiOVXRVgdm51M012aklCeFE" />
 +
<script type="text/javascript" src="https://googledrive.com/host/0B6PQpiOVXRVgR01TNTRVNXUzNGs"></script>
 +
                     
 +
 
 +
 
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    <meta http-equiv="X-UA-Compatible" content="IE=Edge"/><meta http-equiv="Content-Type" content="text/html; charset=utf-8"/><title>NodeFire Save Document</title>
<!-- *** NodeFire copyright (c) 2012, OpenCube Inc. All Rights Reserved. - www.opencube.com
<!-- *** NodeFire copyright (c) 2012, OpenCube Inc. All Rights Reserved. - www.opencube.com
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<style type="text/css">
<style type="text/css">
-
 
-
/*!!!!!!!!!!! NodeFire Core CSS [Do Not Modify!] !!!!!!!!!!!!!*/
 
-
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#qm0 .qmitem-m
 
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{
 
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/*"""""""" (MAIN) Hover State""""""""*/
 
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{
 
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text-decoration:underline;
 
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border-color:#50C1F4;
 
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#qm0 .qmsub
 
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{
 
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padding:2px 5px 0px;
 
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background-color:#313131;
 
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border-color:#50C1F4;
 
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{
 
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                         html {
                         html {
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resize: auto;
resize: auto;
background-color: #001E29;
background-color: #001E29;
 +
                                background-image :  url(https://static.igem.org/mediawiki/2013/3/38/Texture_311_by_sirius_sdz-d5xcdr3.jpg);
 +
                                background-attachment : fixed ;   
 +
                                background-repeat : repeat  ;     
 +
                                background-position : 100% 100% ; 
}
}
                         body {
                         body {
 +
                                margin:2px
background: #001E29;
background: #001E29;
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                                background-image :  url(https://static.igem.org/mediawiki/2013/3/38/Texture_311_by_sirius_sdz-d5xcdr3.jpg);
 +
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 +
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 +
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border-bottom: 0px solid rgb(68, 68, 68);
border-bottom: 0px solid rgb(68, 68, 68);
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            border:0; height:2px; background-color:#99FF9B;
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}
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                      h1, h2, h3, h4, h5, h6 {
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                              font-family: calibri, "Microsoft JhengHei", Times, serif;
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                      font-size: 20pt;
 +
                      color: black;
 +
                      padding-top: 80px;
 +
                     
 +
                              padding-left:425px;
 +
                             
 +
                 
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                      p {
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                          font-famil: calibri, "Microsoft JhengHei", Times, serif;
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<div id='TCULogo'>
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   <a href=""><img src="https://static.igem.org/mediawiki/2013/4/4f/TzuChiU_Formosa_Logo.png" width="975px" height="211px" background-color="rbg (0,30,40)"></a>
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   <a href=""><img src="https://static.igem.org/mediawiki/2013/b/b6/331.png" width="975px" height="210px" background-color="rbg (0,30,40)"></a>
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<font size="4" face="Noticia text" color="white">
 
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<b>TzuChiU Formosa</b>
 
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<li><a class="qmitem-m" href="javascript:void(0)" style="text-align:center;"></a></li>
<li><a class="qmitem-m" href="javascript:void(0)" style="text-align:center;"></a></li>
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<li><a class="qmitem-m" href="https://2013.igem.org/Team:TzuChiU_Formosa" style="text-align:right;">Home</a></li>
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<li><a class="qmitem-m" href="https://2013.igem.org/Team:TzuChiU_Formosa" style="text-align:right;"><span style="font-weight: bold;">Home</span></a></li>
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<li><a class="qmitem-m qmparent" href="https://2013.igem.org/Team:TzuChiU_Formosa/Project">Projects</a>
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<li><a class="qmitem-m qmparent" href="https://2013.igem.org/Team:TzuChiU_Formosa/Project"><span style="font-weight: bold;">Projects</span></a>
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<li><a class="qmitem-s" href="https://2013.igem.org/Team:TzuChiU_Formosa/Safety">Safety</a></li>
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                <li><a class="qmitem-s" href="https://2013.igem.org/Team:TzuChiU_Formosa/Protocol"><span style="font-weight: bold;">Protocol</span></a></li>
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<li><a class="qmitem-s" href="https://2013.igem.org/Week1">Week 1</a></li>
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<li><a class="qmitem-s" href="https://2013.igem.org/Week7"><span style="font-weight: bold;">Week 7</span></a></li>
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<li><a class="qmitem-s" href="https://2013.igem.org/Week8"><span style="font-weight: bold;">Week 8</span></a></li>
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<li><a class="qmitem-m" href="javascript:void(0);">Partners</a></li>
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<li><a class="qmitem-m" href="https://2013.igem.org/Team:TzuChiU_Formosa/Parts;">Parts</a></li>
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<li><a class="qmitem-m" href="https://2013.igem.org/Team:TzuChiU_Formosa/Parts"><span style="font-weight: bold;">Parts</span></a></li>
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<h3><b><u>Monday</b></u></h3>
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<table style="border: 0px hidden rgb(14, 150, 25); height: 150px; background-color: rgb(255, 255, 255); width: 440px; margin-left:80px; margin-top:0px; align="left" cellpadding="5" cellspacing="5" frame="none" rules="ALL">
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<td><font size="4" face="calibri" vertical-align"top">Now that our topic is finalized, we discussed our project’s direction as well as decided on the dates for our periodic meetings where every member should be present.
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We also combined everyone’s forces and did a detailed research for our project. Research included deciding which plasmids to use, methods and protocols, safeties etc.
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<br>
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Once we find all the information we need, we would then select and combine the results at our periodical meetings.
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 +
</tbody>
 +
</table>
 +
 
 +
 
 +
 
 +
<h3><b><u>Tuesday</b></u></h3>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2013/4/42/Week1image2.jpg" width="300px" height="190px" align="left" hspace="70px">
 +
 
 +
<table style="border: 0px hidden rgb(14, 150, 25); height: 150px; background-color: rgb(255, 255, 255); width: 440px; margin-left: 80px; margin-top:0px; align="left" cellpadding="5" cellspacing="5" frame="none" rules="ALL">
 +
<tbody>
 +
<tr>
 +
<td><font size="4" face="calibri" vertical-align"top">Besides continuing to find more information that is related to our project, we have also divided our team into two main groups namely the “Experimental group” and the “ Documental group”
 +
<br>
 +
<br>
 +
The experimental group is responsible for the entire experimental procedure as well as allocating experimental jobs to the team members. The documental group on the other hand is responsible for wiki design, all the paper work and keeping in contact with our advisors and iGEM headquarters.
 +
</font></td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
 
 +
 
 +
 
 +
<h3><b><u>Wednesday</b></u></h3>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2013/b/be/Week1image3.jpg" width="300px" height="190px" align="left" hspace="70px">
 +
 
 +
<table style="border: 0px hidden rgb(14, 150, 25); height: 150px; background-color: rgb(255, 255, 255); width: 440px; margin-left: 80px; margin-top:0px; align="left" cellpadding="5" cellspacing="5" frame="none" rules="ALL">
 +
<tbody>
 +
<tr>
 +
<td><font size="4" face="calibri" vertical-align"top">Luckily, our team members have worked spontaneously and cooperatively together so that we could integrate all our relative information, in order to get our experimental design started. </font></td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
 
 +
 
 +
 +
<h3><b><u>Thursday</b></u></h3>
 +
 
 +
 
 +
<img src="https://static.igem.org/mediawiki/2013/b/b6/Week1image5.jpg" width="300px" height="190px" align="left" hspace="70px">
 +
 
 +
<table style="border: 0px hidden rgb(14, 150, 25); height: 150px; background-color: rgb(255, 255, 255); width: 440px; margin-left: 80px; margin-top:0px; align="left" cellpadding="5" cellspacing="5" frame="none" rules="ALL">
 +
<tbody>
 +
<tr>
 +
<td><font size="4" face="calibri" vertical-align"top">In todays meeting, we all contributed in designing our experiment so that we can give it a kick-start as soon as possible.
 +
<br>
 +
<br>
 +
Some of us contributed in arranging the experiment, some in designing the primers and some in designing the antisense in correlation to our projects antibody and GFP.
 +
</font></td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 +
<h3><b><u>Friday</b></u></h3>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2013/b/b8/W1-5.jpg" width="300px" height="190px" align="left" hspace="70px">
 +
 
 +
<table style="border: 0px hidden rgb(14, 150, 25); height: 150px; background-color: rgb(255, 255, 255); width: 440px; margin-left: 80px; margin-top:0px; align="left" cellpadding="5" cellspacing="5" frame="none" rules="ALL">
 +
<tbody>
 +
<tr>
 +
<td><font size="4" face="calibri" vertical-align"top">Continuing what was left over from the day before, we carried on designing the rest of the experiment, primers and antisense RNA. We also filtered and searched for iGEM biobricks that is necessary for our project.</font></td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
 
 +
 
 +
 
 +
<h3><b><u>Saturday</b></u></h3>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2013/d/df/Week1image4.jpg" width="300px" height="190px" align="left" hspace="70px">
 +
 
 +
<table style="border: 0px hidden rgb(14, 150, 25); height: 150px; background-color: rgb(255, 255, 255); width: 440px; margin-left: 80px; margin-top:0px; align="left" cellpadding="5" cellspacing="5" frame="none" rules="ALL">
 +
<tbody>
 +
<tr>
 +
<td><font size="4" face="calibri" vertical-align"top">After finding all the biobrick parts needed, we started to design the different parts for our plasmid map. These plasmid maps are then to be sent to the headquarters to be used as biobricks.</font></td>
 +
 
 +
 
 +
</tr>
 +
</tbody>
 +
</table>
 +
 
 +
 
 +
 
 +
<h3><b><u>Sunday</b></u></h3>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2013/f/fc/W1-7.jpg" width="300px" height="190px" align="left" hspace="70px">
 +
 
 +
<table style="border: 0px hidden rgb(14, 150, 25); height: 150px; background-color: rgb(255, 255, 255); width: 440px; margin-left: 80px; margin-top:0px; align="left" cellpadding="5" cellspacing="5" frame="none" rules="ALL">
 +
<tbody>
 +
<tr>
 +
<td><font size="4" face="calibri" vertical-align"top">Today the documental group confirmed the materials and E-mailed the headquarters for biobrick. After comparing various biotech companies, we started making phone calls to book the various kits.</font></td>
 +
 
 +
 
 +
</tr>
 +
</tbody>
 +
</table>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
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 +
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 +
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 +
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-
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<font color="black" face="Noticia text" size="6">Monday</FONT><br><br><font color="black" face="Noticia text" size="4">大家好我是星期一謝謝</FONT></td>
 
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  <br>
 
-
  <br>
 
-
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-
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-
  <br>
 
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<table style="border:none; height: 205px; background-color: rgb(255, 255, 255); width:317px;" align="left" cellpadding="1" cellspacing="1" frame="void" rules="none"> 
 
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-
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-
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-
  <br>
 
-
  <br>
 
-
  <font color="black" face="Noticia text" size="6">Tuesday</FONT><br><br><font color="black" face="Noticia text" size="4">大家好我是星期二謝謝</FONT></td>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
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-
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  <hr width="800px">
 
-
      <table style="border:none; height: 205px; background-color: rgb(255, 255, 255); width:317px;" align="left" cellpadding="1" cellspacing="1" frame="void" rules="none"> 
 
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-
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-
      <td><img src="https://static.igem.org/mediawiki/2013/5/5c/Slider_picture_2.png" width="317px"
 
-
height="205px" hspace="40" vspace="20"></td>
 
-
    </tr>
 
-
  </tbody>
 
-
  </table>
 
-
  <br>
 
-
  <br>
 
-
  <font color="black" face="Noticia text" size="6">Wednesday</FONT><br><br><font color="black" face="Noticia text" size="4">大家好我是星期三謝謝</FONT></td>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
  <hr width="800px">
 
-
      <table style="border:none; height: 205px; background-color: rgb(255, 255, 255); width:317px;" align="left" cellpadding="1" cellspacing="1" frame="void" rules="none"> 
 
-
  <tbody>
 
-
    <tr>
 
-
      <td><img src="https://static.igem.org/mediawiki/2013/5/5c/Slider_picture_2.png" width="317px"
 
-
height="205px" hspace="40" vspace="20"></td>
 
-
    </tr>
 
-
  </tbody>
 
-
  </table>
 
-
  <br>
 
-
  <br>
 
-
  <font color="black" face="Noticia text" size="6">Thursday</FONT><br><br><font color="black" face="Noticia text" size="4">大家好我是星期四謝謝</FONT></td>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
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-
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-
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-
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-
  <hr width="800px">
 
-
      <table style="border:none; height: 205px; background-color: rgb(255, 255, 255); width:317px;" align="left" cellpadding="1" cellspacing="1" frame="void" rules="none"> 
 
-
  <tbody>
 
-
    <tr>
 
-
      <td><img src="https://static.igem.org/mediawiki/2013/5/5c/Slider_picture_2.png" width="317px"
 
-
height="205px" hspace="40" vspace="20"></td>
 
-
    </tr>
 
-
  </tbody>
 
-
  </table>
 
-
  <br>
 
-
  <br>
 
-
  <font color="black" face="Noticia text" size="6">Friday</FONT><br><br><font color="black" face="Noticia text" size="4">大家好我是星期五謝謝</FONT></td>
 
-
  <br>
 
-
  <br>
 
-
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-
  <br>
 
-
  <br>
 
-
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-
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-
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-
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-
  <hr width="800px">
 
-
      <table style="border:none; height: 205px; background-color: rgb(255, 255, 255); width:317px;" align="left" cellpadding="1" cellspacing="1" frame="void" rules="none"> 
 
-
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-
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-
      <td><img src="https://static.igem.org/mediawiki/2013/5/5c/Slider_picture_2.png" width="317px"
 
-
height="205px" hspace="40" vspace="20"></td>
 
-
    </tr>
 
-
  </tbody>
 
-
  </table>
 
-
  <br>
 
-
  <br>
 
-
  <font color="black" face="Noticia text" size="6">Saturday</FONT><br><br><font color="black" face="Noticia text" size="4">大家好我是星期六謝謝</FONT></td>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
  <br>
 
-
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-
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-
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-
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-
  <hr width="800px">
 
-
      <table style="border:none; height: 205px; background-color: rgb(255, 255, 255); width:317px;" align="left" cellpadding="1" cellspacing="1" frame="void" rules="none"> 
 
-
  <tbody>
 
-
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-
      <td><img src="https://static.igem.org/mediawiki/2013/5/5c/Slider_picture_2.png" width="317px"
 
-
height="205px" hspace="40" vspace="20"></td>
 
-
    </tr>
 
-
  </tbody>
 
-
  </table>
 
-
  <br>
 
-
  <br>
 
-
  <font color="black" face="Noticia text" size="6">Sunday</FONT><br><br><font color="black" face="Noticia text" size="4">大家好我是星期日謝謝</FONT></td>
 
-
  <br>
 
-
  <br>
 
-
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-
  <br>
 
-
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-
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-
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-
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-
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-
 
 
-
</h3>
 
</body>
</body>
</html>
</html>

Latest revision as of 00:03, 28 September 2013

TzuChiU Formosa NodeFire Save Document






Monday

Now that our topic is finalized, we discussed our project’s direction as well as decided on the dates for our periodic meetings where every member should be present.

We also combined everyone’s forces and did a detailed research for our project. Research included deciding which plasmids to use, methods and protocols, safeties etc.

Once we find all the information we need, we would then select and combine the results at our periodical meetings.

Tuesday

Besides continuing to find more information that is related to our project, we have also divided our team into two main groups namely the “Experimental group” and the “ Documental group”

The experimental group is responsible for the entire experimental procedure as well as allocating experimental jobs to the team members. The documental group on the other hand is responsible for wiki design, all the paper work and keeping in contact with our advisors and iGEM headquarters.

Wednesday

Luckily, our team members have worked spontaneously and cooperatively together so that we could integrate all our relative information, in order to get our experimental design started.

Thursday

In todays meeting, we all contributed in designing our experiment so that we can give it a kick-start as soon as possible.

Some of us contributed in arranging the experiment, some in designing the primers and some in designing the antisense in correlation to our projects antibody and GFP.



Friday

Continuing what was left over from the day before, we carried on designing the rest of the experiment, primers and antisense RNA. We also filtered and searched for iGEM biobricks that is necessary for our project.

Saturday

After finding all the biobrick parts needed, we started to design the different parts for our plasmid map. These plasmid maps are then to be sent to the headquarters to be used as biobricks.

Sunday

Today the documental group confirmed the materials and E-mailed the headquarters for biobrick. After comparing various biotech companies, we started making phone calls to book the various kits.

Retrieved from "http://2013.igem.org/Week1"