Team:Uppsala/test

From 2013.igem.org

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<!DOCTYPE html>
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{{Team:Uppsala/standard-style}}
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{{Team:Uppsala/index-style}}
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{{Team:Uppsala/calender-test}}
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{{Team:Uppsala/favicon}}
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<html>
<html>
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<title>Uppsala iGEM 2013</title>
<title>Uppsala iGEM 2013</title>
<link rel="shortcut icon" href="favicon.ico" type="image/x-icon">
<link rel="shortcut icon" href="favicon.ico" type="image/x-icon">
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<link rel="stylesheet" type="text/css" href="css/style.css">
 
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<meta http-equiv="Content-Type" charset="utf-8" />
 
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<title>Uppsala iGEM 2013</title>
 
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<link rel="shortcut icon" href="favicon.ico" type="image/x-icon">
 
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<link rel="stylesheet" type="text/css" href="css/styles.css">
 
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<link rel="stylesheet" type="text/css" href="css/calendar.css" media="all" />
 
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<script type="text/javascript" src="script/calendar.js"></script>
 
<style type="text/css">
<style type="text/css">
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</style>
</style>
<script type="text/javascript">
<script type="text/javascript">
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/*Acts once the document is loaded*/ $(document).ready(function() { /*Move the tn-main-wrap as child of <body>*/ $("#tn-main-wrap-wrap").prependTo($("body")); /*Move the menubars in the tn-main-wrap*/ $("#menubar.left-menu").appendTo($("#tn-main-wrap")); $("#menubar.right-menu").appendTo($("#tn-main-wrap"));
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var month = new Date().getMonth(); 
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/*Spoiler JS*/ $(".tn-spoiler div").slideUp(); $(".tn-spoiler a").click(function(e) { e.preventDefault(); $(".tn-spoiler-active").removeClass("tn-spoiler-active"); $(this).parent().addClass("tn-spoiler-active"); $(".tn-spoiler").not(".tn-spoiler-active").children("div").slideUp(); $(".tn-spoiler-active").children("div").slideToggle(); });
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var year = new Date().getFullYear(); 
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/*Add favicon*/ $("link[rel='shortcut icon']").remove(); $("head").append("<link rel='shortcut icon' type='image/png' href='https://static.igem.org/mediawiki/2013/6/6d/Uppsalas-cow-con.png'>"); });
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var cal_table = '';   
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var eventString;
+
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var days_with_dirys = new Array('t201381');
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var inputs = 1;
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var days_in_month = new Array(31,28,31,30,31,30,31,31,30,31,30,31);
+
-
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/*getWeek() will calculate current week nr*/  
+
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Date.prototype.getWeek = function()  
+
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{
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  var onejan = new Date(this.getFullYear(),0,1);
+
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  return Math.ceil((((this - onejan) / 86400000) + onejan.getDay()+1)/7);
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}
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+
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/*writes/rewrites the calculator string */  
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function calendar_normal()
+
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{
+
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  var m = month+1;
+
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  var date = new Date();   
+
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  var today = date.getDate();   
+
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  cal_table = ''; 
+
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+
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  if(year<=200)
+
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  {
+
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    year += 1900;
+
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  }
+
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  months = new Array('January', 'February', 'March', 'April', 'May', 'June', 'July', 'August', 'September', 'October', 'November', 'December');
+
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  var weekDay = new Array('Mon', 'Tue', 'We', 'Thu', 'Fri', 'Sat', 'Sun');
+
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  days_in_month = new Array(31,28,31,30,31,30,31,31,30,31,30,31);
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if(year%4 == 0 && year!=1900)
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  {
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  days_in_month[1]=29;
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}
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+
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+
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    total = days_in_month[month];
+
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    var date_today = months[month]+' '+year;
+
-
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    cal_table = cal_table + '<table class="cal_calendar" ><tbody id="cal_body"><tr class="head"><th class="arrow"><input id="left" type="image" onclick="left_arrow()" src="https://static.igem.org/mediawiki/2013/a/a1/Arrowleft.png"></th><th colspan="6" >'+date_today+'</th><th class="arrow"><input id="right" type="image" onclick="right_arrow()" src="https://static.igem.org/mediawiki/2013/0/0b/Arrowright.png"></th></tr>';
+
-
    cal_table = cal_table + '<tr class="cal_d_weeks"><th>Mon</th><th>Tue</th><th>Wed</th><th>Thu</th><th>Fri</th><th>Sat</th><th>Sun</th><th>Week</th></tr><tr>';
+
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    week = 0;
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    var first_week_day = new Date(year, month, 0).getDay();
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    for(week_day = 0; week_day < first_week_day; week_day++)
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    {
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      cal_table = cal_table +'<td class="cal_days_bef_aft"></td>';
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      week++;
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    }
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for(i=1;i<=total;i++)
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    {
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        if(week===0)
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        {
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            cal_table = cal_table + '<tr>';
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        }
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        if(today===i && month == date.getMonth() && year == date.getFullYear())
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        {
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            cal_table = cal_table +'<td id="'+'t'+year+m+i+'" class="cal_today"><input type="submit" onclick="showNotes(year, month, value)" value="'+i+'"></td>';
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        }
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        else
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        {
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            cal_table = cal_table +'<td id="'+'t'+year+m+i+'" class="not_today"><input type="submit" onclick="showNotes(year, month, value)" value="'+i+'"></td>';
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        }
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        week++;
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  markerNotes(year, m, i);
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        if(week==7)
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        {
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  var weeknr = new Date(year, month, i-1).getWeek();
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  cal_table = cal_table + '<td class="week"><input type="submit" onclick="showNotesOfweek(year, value)" value="'+weeknr+'"></td>';
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        cal_table = cal_table + '</tr>';   
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  week=0;
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        }
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        }
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        for(i=1;week!=0;i++)
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        {
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                cal_table = cal_table + '<td class="cal_days_bef_aft"></td>';
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                week++;
+
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                if(week==7)
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                { 
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      if(weeknr == 53)
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      {
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      var weeknr = 1;
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      }
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      else
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      {
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      var weeknr = weeknr+1;
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      }
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      cal_table = cal_table + '<td class="week"><input type="submit" onclick="showNotesOfweek(year, value)" value="'+weeknr+'"></td>';
+
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                        cal_table = cal_table + '</tr>';
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                        week=0;
+
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                }
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        }
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        cal_table = cal_table + '</tbody></table>';
+
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}
+
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+
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function left_arrow()
+
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{
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month = month-1;
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  if(month == -1)
+
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  {
+
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  year = year - 1;
+
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  month = 11;
+
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  }
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calendar_normal();
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document.getElementById('calendar').innerHTML = cal_table;
+
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}
+
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+
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function right_arrow()
+
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{
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month = month+1;
+
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  if(month == 12)
+
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  {
+
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  year = year +1;
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  month = 0;
+
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  } 
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calendar_normal();
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document.getElementById('calendar').innerHTML = cal_table;
+
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}
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+
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function showNotes(y, m, day)
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{
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  var m = month+1;
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-
+
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  eventString =  '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';
+
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  document.getElementById('file_holder').innerHTML = eventString;
+
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}
+
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+
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function showNotesOfweek(y, wnr)
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{
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  var d = new Date(y,0,0);
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  if(y == 2012 ){
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wnr++;
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}
+
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  var w = d.getTime() + 604800000 * (wnr-1);
+
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  var m = new Date(w).getMonth()+1;
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  var y = new Date(w).getFullYear();
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  var day = new Date(w).getDate();
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-
 
+
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  var e = new Date(w).getDay();
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  day = day - e+1;
+
-
 
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  eventString = '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';
+
-
 
+
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  for(i=1;i<7;i++)
+
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  {
+
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    if(days_in_month[m-1] == day )
+
-
    {
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    day = 0;
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    m++;
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    if(m>12)
+
-
    {
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      y++;
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      var d = new Date(y, 0, 0);
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-
      var w = d.getTime() + 604800000 * (wnr-1);
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-
      var m = 1;
+
-
    }
+
-
   
+
-
    }
+
-
    day++;
+
-
    eventString = eventString + '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';
+
-
  }
+
-
  document.getElementById('file_holder').innerHTML = eventString;
+
-
  eventString = '';
+
-
}
+
-
 
+
-
function markerNotes(y, m, d)
+
-
{
+
-
var id='t'+y+m+d;
+
-
 
+
-
for(c = 0; c<=inputs; c++)
+
-
{
+
-
  if(days_with_dirys[c] == id)
+
-
  {
+
-
  cal_table = cal_table + '<style type="text/css">#'+id+'{border: 2px solid #FFA500;}</style>';
+
-
  }
+
-
}
+
-
}
+
-
 
+
-
function diry(y, m, d)
+
-
{
+
-
var ds = '';  //diryString
+
-
var id = 'd'+y+m+d;
+
-
//var ds = y+'-'+m+'-'+d;
+
-
if(id == 'd201381')
+
-
{
+
-
  ds = '<h2>Thursday 2013-08-01</h2><p>Name of participants: Marcus H, Karl H, Lovisa P, Emil M, Victor S, Hampus.E<br><br><h3>Ongoing constructs:</h3><br>' +
+
-
  '114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>'+
+
-
  '137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>'+
+
-
  '187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23101-B0034-TAL<br>'+
+
-
 
+
-
  '<h3>Todays work</h3><br><h3>PCR screening and gel electrophorersis of construct:</h3><br>185.(5-10) pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>'+
+
-
 
+
-
  '<h3>sequencing prep. of constructs:</h3><br>185.9-185.10<br>'+
+
-
 
+
-
  '<h3>Transformation of constructs:</h3><br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23101-B0034-TAL<br><br>'+
+
-
 
+
-
  '<h3>Results</h3><br>Construct 185, clone 9 and 10, bonds around 3000/4000 bp.<br><br>'+
+
-
 
+
-
  '<h3>Follow up</h3><br>- <br><br>'+
+
-
 
+
-
  '<h3>Other experiments</h3><br>-<br></p>';
+
-
}
+
-
else
+
-
{
+
-
  ds = 'Nothing happend during this day!';
+
-
}
+
-
document.getElementById(id).setAttribute('onclick','closeNote('+y+', '+m+', '+d+');');
+
-
document.getElementById(id).innerHTML = ds;
+
-
}
+
-
 
+
-
function closeNote(y, m, d)  
+
-
{
+
-
var ds = '';  
+
-
var id = 'd'+y+m+d;
+
-
var ds = '<h1>'+y+'-'+m+'-'+d+'</h1>';
+
-
document.getElementById(id).setAttribute('onclick','diry('+y+', '+m+', '+d+');');
+
-
document.getElementById(id).innerHTML = ds;
+
-
}
+
-
 
+
-
 
+
</script>
</script>
-
 
</head>
</head>
Line 268: Line 30:
<!-- Contains sub-logo picture -->
<!-- Contains sub-logo picture -->
-
<div id="igem_logo"><a href="https://2013.igem.org/Main_Page"><img class="igem_logo" src="https://static.igem.org/mediawiki/2013/5/59/IGEMlogo.png"></a></div>
+
<div id="igem_logo"><a href="https://2013.igem.org/Team:Uppsala"><img class="igem_logo" src="https://static.igem.org/mediawiki/2013/e/e0/Uppsala2013_IGEMlogo.png"></a></div>
-
<div id="igem"><a href="https://2013.igem.org/Main_Page"><img class="igem" src="https://static.igem.org/mediawiki/2013/9/9c/Uppsala_IGEM_log_blue.png"></a></div>
+
<div id="igem"><a href="https://2013.igem.org/Main_Page"><img class="igem" src="https://static.igem.org/mediawiki/2013/b/b8/Uppsala2013_Uppsala_IGEM_log_blue.png"></a></div>
 +
 
 +
<img class="slogan" src="https://static.igem.org/mediawiki/2013/a/ad/Uppsala2013_Header.png">
</div>  
</div>  
Line 281: Line 45:
<div id="nav">
<div id="nav">
-
<div id="cow-box"><a href="https://2013.igem.org/Team:Uppsala"><img class="home-knapp" src="https://static.igem.org/mediawiki/2013/9/9a/Home.png"><a/></div>
+
<div id="cow-box"><a href="https://2013.igem.org/Team:Uppsala"><img class="home-knapp" src="https://static.igem.org/mediawiki/2013/e/ef/Uppsala2013_Home.png"><a/></div>
<ul>
<ul>
-
<li><a href="https://2013.igem.org/Team:Uppsala/project" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/9/92/Project.png"></a>
+
<li><a href="https://2013.igem.org/Team:Uppsala/project" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/a/aa/Uppsala2013_Project.png"></a>
<ul>
<ul>
-
<li><a href="https://2013.igem.org/Team:Uppsala/overview">Overwiev</a></li>
+
-
<li><a href="#">Chassi</a>
+
<li><a href="https://2013.igem.org/Team:Uppsala/chassi">Chassi</a>
<ul>
<ul>
 +
                                                                <li><a href="https://2013.igem.org/Team:Uppsala/probiotics">Probiotics</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/promoters">Promoters</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/promoters">Promoters</a></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/reporterg-enes">Reporter genes</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/reporter-genes">Reporter genes</a></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/affinity-tags">Affinity tags</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/toxin-antitoxin-system">Toxin-antitoxin system</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/vectors">Vectors</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/vectors">Vectors</a></li>
 +
                                                                <li><a href="https://2013.igem.org/Team:Uppsala/signal-peptide">Signal peptide</a></li>
</ul></li>
</ul></li>
                                                  
                                                  
-
                                                 <li><a href="#">Metabolic engineering</a>
+
                                                 <li><a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering">Metabolic engineering</a>
                                                     <ul>
                                                     <ul>
-
                                                                 <li><a href="#">Resveratrol</a></li>
+
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid">P-coumaric acid</a></li>
 +
                                                                <li><a href="https://2013.igem.org/Team:Uppsala/resveratrol">Resveratrol</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/betacarotene">β-carotene</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/betacarotene">β-carotene</a></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/astraxantin">Astraxantin</a></li>
+
                                                                <li><a
-
<li><a href="https://2013.igem.org/Team:Uppsala/zeaxantin">Zeaxantin</a></li>
+
href="https://2013.igem.org/Team:Uppsala/saffron">Saffron</a></li>
 +
<li><a href="https://2013.igem.org/Team:Uppsala/astaxanthin">Astaxanthin</a></li>
 +
<li><a href="https://2013.igem.org/Team:Uppsala/zeaxanthin">Zeaxanthin</a></li>
</ul></li>
</ul></li>
</li>  
</li>  
-
<li><a href="#">Chromoprotein</a></li>
+
                                                <li><a
-
<li><a href="#">Safety experiments</a></li>
+
href="https://2013.igem.org/Team:Uppsala/miraculin">Miraculin</a></li>
-
<li><a href="#">Result</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/chromoproteins">Chromoproteins</a></li>
 +
<li><a href="https://2013.igem.org/Team:Uppsala/safety-experiment">Safety experiment</a></li>
 +
<li><a href="https://2013.igem.org/Team:Uppsala/results">Results</a></li>
</ul></li>
</ul></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/3/3c/Modeling.png"></a>
+
<li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/6/63/Uppsala2013_Modeling.png"></a>
<ul>
<ul>
-
<li><a href="#">Resveratrol pathway </a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/P-Coumaric-acid-pathway">P-Coumaric acid</a></li>
-
<li><a href="#">Carotenoids pathway </a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/modeling-tutorial">Modeling tutorial </a></li>
</ul></li>
</ul></li>
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Making the world healthier with a new probiotic platform
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<p class="project-text"> Malnutrition is today a major global problem that affects people both in affluent and developing countries. Even if you get the right amount of calories, if these do not contain sufficient amounts of micronutrients, like vitamins and minerals, serious illness and even death can be the result.
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The goal of our project is to alleviate this problem by applying synthetic biology to <a href="https://2013.igem.org/Team:Uppsala/probiotics">probiotic bacteria</a>. These bacteria are already a part of normal human diet and our goal is to modify them so that they enrich the nutritional content of the food they grow in.<br><br>
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With our project, we will make the lactobacillus genus the <a href="https://2013.igem.org/Team:Uppsala/chassi"> new probiotic platform </a> for <a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering"> metabolic engineering </a> of nutritional compounds. We will engineer probiotics to produce beta-carotene (provitamin), resveratrol (antioxidant), miraculin (sweetener) and saffron (taste).
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To exemplify what this combination of probiotics and metabolic engineering can accomplish we hope to use our modified bacteria to create nutritionally enriched yoghurt. We have also put great effort into addressing the <a href="https://2013.igem.org/Team:Uppsala/biosafety-and-ethics"> ethical and safety </a> issues that naturally follow when creating genetically modified food. As a part of this we organised a large event, <a href="https://2013.igem.org/Team:Uppsala/synbioday"> Uppsala SynBioDay </a>, with over 200 participants where these issues were discussed.
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Latest revision as of 15:12, 27 October 2013

Making the world healthier with a new probiotic platform

Malnutrition is today a major global problem that affects people both in affluent and developing countries. Even if you get the right amount of calories, if these do not contain sufficient amounts of micronutrients, like vitamins and minerals, serious illness and even death can be the result. The goal of our project is to alleviate this problem by applying synthetic biology to probiotic bacteria. These bacteria are already a part of normal human diet and our goal is to modify them so that they enrich the nutritional content of the food they grow in.

With our project, we will make the lactobacillus genus the new probiotic platform for metabolic engineering of nutritional compounds. We will engineer probiotics to produce beta-carotene (provitamin), resveratrol (antioxidant), miraculin (sweetener) and saffron (taste). To exemplify what this combination of probiotics and metabolic engineering can accomplish we hope to use our modified bacteria to create nutritionally enriched yoghurt. We have also put great effort into addressing the ethical and safety issues that naturally follow when creating genetically modified food. As a part of this we organised a large event, Uppsala SynBioDay , with over 200 participants where these issues were discussed.

Retrieved from "http://2013.igem.org/Team:Uppsala/test"