Team:TU-Delft/Notebook/2013/08/22/

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<p> 1. pBAD:Agr AC:pP2 <br>  
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<p> 1. pBAD:Agr AC:pP2, restriction digested back bone of <i> B. subtilis </i>, AIP:GFP:TT was run on gel electrophoresis.  <br>  
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2. Carried out colony PCR on His-SUMO peptides with correct primer for pET23B and ran on gel. This gel gave good results. <br>
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2. Inoculation of pBAD(BBa_K20600) from glycerol stock in LB broth with chloroamphenicol resistance. <br>
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3. Inoculation of pTET:RBS:TT. <br>
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4. Sent samples of pBAD:AgrAC:pP2 from colony 3 and colony 7 for sequencing. <br>
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5. MIC determination experiment was carried out on <i> E.coli, S.delphini and B.subtilis</i> by inducing with 'Signiferin' peptide. The peptide was found to stop only the growth of <i> S.delphini and B.subtilis</i>. <br>  
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3. Transformation of pBAD:AgrAC:pP2 was done in XL1 Blue cells and plated on agar plates. <br>
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4. PCR purification of His-SUMO-Peptides was done. <br>
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5. 10X TBE buffer was made and kept in stock. <br>
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Latest revision as of 08:58, 4 October 2013

Notebook

June
SunMonTueWedThuFriSat
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30
July
SunMonTueWedThuFriSat
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31










August
SunMonTueWedThuFriSat
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
September
SunMonTueWedThuFriSat
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30













22nd of August


Lab work

1. pBAD:Agr AC:pP2, restriction digested back bone of B. subtilis , AIP:GFP:TT was run on gel electrophoresis.




2. Inoculation of pBAD(BBa_K20600) from glycerol stock in LB broth with chloroamphenicol resistance.
3. Inoculation of pTET:RBS:TT.
4. Sent samples of pBAD:AgrAC:pP2 from colony 3 and colony 7 for sequencing.
5. MIC determination experiment was carried out on E.coli, S.delphini and B.subtilis by inducing with 'Signiferin' peptide. The peptide was found to stop only the growth of S.delphini and B.subtilis.

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