Team:Paris Saclay/Notebook/August/23

From 2013.igem.org

(Difference between revisions)
(Created page with "{{Team:Paris_Saclay/incl_debut_generique}} ='''Notebook : August 23'''= =='''Lab work'''== ==='''A - Aerobic/Anaerobic regulation system'''=== ===='''Objective : obtaining Pf...")
Line 9: Line 9:
===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3'''====
===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3'''====
-
===='''1 - Electrophoresis to check the digestion of Bba_J04450 by EcoRI/PstI'''====
+
(((((((((((((((===='''1 - Electrophoresis to check the digestion of Bba_J04450 by EcoRI/PstI'''====
{|
{|
Line 25: Line 25:
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
We can see anything. The digestion did work.
We can see anything. The digestion did work.
-
|}
+
|}))))))))))))))))))))))
{{Team:Paris_Saclay/incl_fin}}
{{Team:Paris_Saclay/incl_fin}}

Revision as of 22:57, 17 September 2013

Contents

Notebook : August 23

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3

(((((((((((((((====1 - Electrophoresis to check the digestion of Bba_J04450 by EcoRI/PstI====

]]
  • Well 1 : 6µL DNA Ladder
  • Well 2 : 5µL of Bba_J04450 digested by EcoRI/PstI+1µl of 6X loading dye
  • Gel : 1%

Expect sizes :

  • PSB3K3 : 2750 bp

We can see anything. The digestion did work.

))))))))))))))))))))))