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Team:Macquarie Australia/Notebook
From 2013.igem.org
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<td></td><td><span style="color:#8B0000"><font size = 3><a class="three" href='https://2013.igem.org/Team:Macquarie_Australia/Results'><b>Here</b></a></font size></span>. | <td></td><td><span style="color:#8B0000"><font size = 3><a class="three" href='https://2013.igem.org/Team:Macquarie_Australia/Results'><b>Here</b></a></font size></span>. | ||
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| + | <p>We spent this week designing the sequences of the genes: ChlI1, CTH1, CHlM, POR, CHlD, ChlH, GUN4 and Plastocyanin that we will use for the project, including gBlocks and PCR products. The gBlocks were designed to eliminate unwanted restriction endonuclease sites, encode restriction enzyme sites EcoR1 (E), Xbal (X), SpeI (S) and PstI (P) at the ends of the gBlocks and possess 35bp overlaps between the gBlocks and PCR products that comprise each gene separately. Each gene sequence was ensured to have a bacterial ribosome binding site (shine-dalgarno sequence).</p> | ||
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| + | 1. ChlI1 - 1 gBlock + 2 PCR products<br> | ||
| + | 2. ChlH - 5 gBlocks + 3 PCR products<br> | ||
| + | 3. ChlD - 1 gBlock + 2 PCR products<br> | ||
| + | 4. GUN4 - 1 gBlock + 1 PCR product<br> | ||
| + | 5. ChlM - 1 gBlock + 1 PCR product<br> | ||
| + | 6. CTH1 - 2 gBlocks + 1 PCR products<br> | ||
| + | 7. POR - 1 gBlock + 1 PCR product<br> | ||
| + | 8. Plastocyanin - 1 gBlock<br> | ||
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Revision as of 02:07, 12 September 2013
Notebook
To browse through our notebook simply click on the image with the date. It will expand with what we did that week. To continue on simply click on the next one. If the notebook isn't functioning as planned (it will not load the next tab of content) then please go to our linearised view which is located here.
A seperate
Intro to the week, Work being done, "Using PCR we amplified the genes X and X"
Results (This worked, this didnt), "Gene X didnt amplify, Gene X did"
Small discussion + Whats next "We plan on then doing X to amplified gene"
We spent this week designing the sequences of the genes: ChlI1, CTH1, CHlM, POR, CHlD, ChlH, GUN4 and Plastocyanin that we will use for the project, including gBlocks and PCR products. The gBlocks were designed to eliminate unwanted restriction endonuclease sites, encode restriction enzyme sites EcoR1 (E), Xbal (X), SpeI (S) and PstI (P) at the ends of the gBlocks and possess 35bp overlaps between the gBlocks and PCR products that comprise each gene separately. Each gene sequence was ensured to have a bacterial ribosome binding site (shine-dalgarno sequence).
1. ChlI1 - 1 gBlock + 2 PCR products2. ChlH - 5 gBlocks + 3 PCR products
3. ChlD - 1 gBlock + 2 PCR products
4. GUN4 - 1 gBlock + 1 PCR product
5. ChlM - 1 gBlock + 1 PCR product
6. CTH1 - 2 gBlocks + 1 PCR products
7. POR - 1 gBlock + 1 PCR product
8. Plastocyanin - 1 gBlock
With the break between semesters over, the Macquarie iGEM team returned to classes; for many of us this was the first day of iGEM 2013. We had our first meeting and discussed our project. Dr. Louise Brown and Associate Professor Rob Willows were introduced to the team and we began to determine who would take on certain roles within the team.
We eagerly began our project, deciding to use Gibson Assembly to produce our optimised genes. We had decided to develop BioBricks necessary for the biosynthesis of chlorophyll within E.coli. To do this, we constructed a gene pathway, containing 12 genes to reach our goal of chlorophyll biosynthesis.
Week 2 (G block assemblies & transformations) - Update Coming soon
