Week6

From 2013.igem.org

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<li><a class="qmitem-s" href="https://2013.igem.org/Team:TzuChiU_Formosa/Overview"><span style="font-weight: bold;">Overview</span></a></li>
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                 <li><a class="qmitem-s" href="https://2013.igem.org/Team:TzuChiU_Formosa/Protocol"><span style="font-weight: bold;">Protocol</span></a></li>
                 <li><a class="qmitem-s" href="https://2013.igem.org/Team:TzuChiU_Formosa/Protocol"><span style="font-weight: bold;">Protocol</span></a></li>
                 <li><a class="qmitem-s" href="https://2013.igem.org/Team:TzuChiU_Formosa/Result"><span style="font-weight: bold;">Result</span></a></li>
                 <li><a class="qmitem-s" href="https://2013.igem.org/Team:TzuChiU_Formosa/Result"><span style="font-weight: bold;">Result</span></a></li>
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<h3><b><u>Monday</b></u></h3>
<h3><b><u>Monday</b></u></h3>
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<img src="https://static.igem.org/mediawiki/2013/6/60/Slide_picture_1.png" width="300px" height="190px" align="left" hspace="70px">
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<td><font size="4" face="calibri" vertical-align"top">We were given the opportunity to select members from various faculties to compose our own iGEM team. Qualities of our Hypnoseq member should be adventurous, committed and have a passion for research. </font></td>
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<td><font size="4" face="calibri" vertical-align"top">A start of a new week but the middle of our experiment. Digestion includes PSB1C3 from our iGEM part. Through gel electrophoresis we can confirm that we are on the right track and via gel extraction we are able to extract our PSB1C3 vector. Unfortunately, the OD value showed that our plasmid concentration is too low! We are not entirely sure what went wrong, is it that we did not follow the protocol correctly? Or is there something wrong with the kits? :/ </font></td>
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<h3><b><u>Tuesday</b></u></h3>
<h3><b><u>Tuesday</b></u></h3>
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<img src="https://static.igem.org/mediawiki/2013/5/5c/Slider_picture_2.png" width="300px" height="190px" align="left" hspace="70px">
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<img src="https://static.igem.org/mediawiki/2013/3/35/Week6-2.jpg" width="300px" height="190px" align="left" hspace="70px">
<table style="border: 0px hidden rgb(14, 150, 25); height: 150px; background-color: rgb(255, 255, 255); width: 440px; margin-left: 80px; margin-top:0px; align="left" cellpadding="5" cellspacing="5" frame="none" rules="ALL">
<table style="border: 0px hidden rgb(14, 150, 25); height: 150px; background-color: rgb(255, 255, 255); width: 440px; margin-left: 80px; margin-top:0px; align="left" cellpadding="5" cellspacing="5" frame="none" rules="ALL">
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<td><font size="4" face="calibri" vertical-align"top">After our team was constructed, our members came up with a few possible ideas/ projects for the competition. Basic researches were done to support the ideas for future selection. </font></td>
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<td><font size="4" face="calibri" vertical-align"top">Repeating yesterdays experiment, we have failed once again  We have tried kits from different labs but the results displayed are very much the same. Though the photo of the gel looks beautiful but the concentration is still too low!
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What do we dooo……?!
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<h3><b><u>Wednesday</b></u></h3>
<h3><b><u>Wednesday</b></u></h3>
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<img src="https://static.igem.org/mediawiki/2013/1/1f/Slide_picture_3.png" width="300px" height="190px" align="left" hspace="70px">
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<img src="https://static.igem.org/mediawiki/2013/9/98/Week6-3.jpg" width="300px" height="190px" align="left" hspace="70px">
<table style="border: 0px hidden rgb(14, 150, 25); height: 150px; background-color: rgb(255, 255, 255); width: 440px; margin-left: 80px; margin-top:0px; align="left" cellpadding="5" cellspacing="5" frame="none" rules="ALL">
<table style="border: 0px hidden rgb(14, 150, 25); height: 150px; background-color: rgb(255, 255, 255); width: 440px; margin-left: 80px; margin-top:0px; align="left" cellpadding="5" cellspacing="5" frame="none" rules="ALL">
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<td><font size="4" face="calibri" vertical-align"top">Our team members then presented the ideas/ projects at our campus iGEM preliminary round. The possible ideas were then assessed and voted by our advisor and peers.</font></td>
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<td><font size="4" face="calibri" vertical-align"top">For the past few days we have been doing digestion and gel electrophoresis like a mad dog. Today, we have dedicated ourselves to plasmid extraction! To learn from our lesson, this time we extracted an entire rack so that we will have sufficient PSB1C3 vector for future use.</font></td>
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<img src="https://static.igem.org/mediawiki/2013/6/60/Slide_picture_4.png" width="300px" height="190px" align="left" hspace="70px">
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<img src="https://static.igem.org/mediawiki/2013/e/eb/Weel6-4.jpg" width="300px" height="190px" align="left" hspace="70px">
<table style="border: 0px hidden rgb(14, 150, 25); height: 150px; background-color: rgb(255, 255, 255); width: 440px; margin-left: 80px; margin-top:0px; align="left" cellpadding="5" cellspacing="5" frame="none" rules="ALL">
<table style="border: 0px hidden rgb(14, 150, 25); height: 150px; background-color: rgb(255, 255, 255); width: 440px; margin-left: 80px; margin-top:0px; align="left" cellpadding="5" cellspacing="5" frame="none" rules="ALL">
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<td><font size="4" face="calibri" vertical-align"top">Out team members went to a few junior high schools to promote synthetic biology to the scholars. Surveys were also handed out after the presentation so we have an overall perception of their understanding of synthetic biology.</font></td>
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<td><font size="4" face="calibri" vertical-align"top">Our experiment is running downhill again!! We are still trying various ways to put things on track, continuously running gel electrophoresis and cutting gel and running gel electrophoresis and cutting gel and asking our advisors as well as seniors, what may go wrong?! But the concentration is still very low…
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Our GFP generator has arrived from the iGEM foundation. Still nothing grew, not a tint of bacterium  We have again encountered another problem.  
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Doesn't matter! Never give up! We will continue to try different ways to make it work!
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</font></td>
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Revision as of 07:53, 27 September 2013

TzuChiU Formosa NodeFire Save Document









Monday

A start of a new week but the middle of our experiment. Digestion includes PSB1C3 from our iGEM part. Through gel electrophoresis we can confirm that we are on the right track and via gel extraction we are able to extract our PSB1C3 vector. Unfortunately, the OD value showed that our plasmid concentration is too low! We are not entirely sure what went wrong, is it that we did not follow the protocol correctly? Or is there something wrong with the kits? :/

Tuesday

Repeating yesterdays experiment, we have failed once again  We have tried kits from different labs but the results displayed are very much the same. Though the photo of the gel looks beautiful but the concentration is still too low! What do we dooo……?!

Wednesday

For the past few days we have been doing digestion and gel electrophoresis like a mad dog. Today, we have dedicated ourselves to plasmid extraction! To learn from our lesson, this time we extracted an entire rack so that we will have sufficient PSB1C3 vector for future use.

Thursday

Our experiment is running downhill again!! We are still trying various ways to put things on track, continuously running gel electrophoresis and cutting gel and running gel electrophoresis and cutting gel and asking our advisors as well as seniors, what may go wrong?! But the concentration is still very low… Our GFP generator has arrived from the iGEM foundation. Still nothing grew, not a tint of bacterium  We have again encountered another problem. Doesn't matter! Never give up! We will continue to try different ways to make it work!

Friday

After our direction was set and our goal was clear, we started to construct our iGEM webpage, filling in related details and keeping our records up to date.

Saturday

In order to save time, we did some basic preparations for the experiment before all the parts were received. Preparations include primer design, preparing LB plates, making competent cells etc. so when the biobrick is received the actual experiment may start immediately.

Sunday

In order to save time, we did some basic preparations for the experiment before all the parts were received. Preparations include primer design, preparing LB plates, making competent cells etc. so when the biobrick is received the actual experiment may start immediately.

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