Team:Paris Saclay/Notebook/August/29

From 2013.igem.org

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B1-8: promoter fnr(activator)nirB plus RBS_AmilCP+Term_PSB1C3 </u>
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|PST I
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|2µl
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Revision as of 16:10, 12 September 2013

Navigation Home Team Project Labwork Notebook Human practices

Notebook : August 29

summary

  • We got clonies on ligation promoter fnr(activator)nirB plus RBS_LacZ+Term_PSB1C3

promoter fnr(activator)nirB plus RBS_AmilCP+Term_PSB1C3 promoter fnr(repressor) plus RBS_LacZ+Term_PSB1C3 promoter fnr(repressor) plus RBS_AmilCP+Term_PSB1C3t so we do Colony PCR for it

  • We didn't get clonies on Gibson so we do a gel electrophoresis of parts of Gibson assembly to verify the size
  • Digestion Dnp1 purification of the the PSB1C3 clean for Gibson and electrophoresis of it .


lab work


  • A.aero/anaerobic regulation system


Digestion for PSB3K3



DNA 9µl
Ecoil I 2µl
PST I 2µl
H2O 5µl
buffer 2µl
total 20µl
Digestion for RBS_LacZ+Term_10(08/09/13)

B1-8: promoter fnr(activator)nirB plus RBS_AmilCP+Term_PSB1C3


DNA 14µl
xpe I 2µl
PST I 2µl
buffer 2µl
total 20µl
Digestion for RBS_LacZ+Term_11(08/09/13)

B1-8: promoter fnr(activator)nirB plus RBS_AmilCP+Term_PSB1C3


DNA 14µl
xpe I 2µl
PST I 2µl
buffer 2µl
total 20µl
Digestion for RBS_LacZ+Term_15(08/09/13)

B1-8: promoter fnr(activator)nirB plus RBS_AmilCP+Term_PSB1C3


DNA 14µl
xpe I 2µl
PST I 2µl
buffer 2µl
total 20µl


Digestion for RBS_AmilCP+Term_9(02/09/13)



DNA 14µl
xpe I 2µl
PST I 2µl
buffer 2µl
total 20µl
Digestion for RBS_AmilCP+Term_12(02/09/13)



DNA 14µl
xpe I 2µl
PST I 2µl
buffer 2µl
total 20µl
Retrieved from "http://2013.igem.org/Team:Paris_Saclay/Notebook/August/29"