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Team:Macquarie Australia/Protocols/PCR
From 2013.igem.org
(Difference between revisions)
| Line 24: | Line 24: | ||
Initial denaturation 98°C for 30 secs<br> | Initial denaturation 98°C for 30 secs<br> | ||
<b>Followed by 30 repeats of:</b><br> | <b>Followed by 30 repeats of:</b><br> | ||
| - | Denaturation 98 °C 10 secs<br> | + | Denaturation - 98 °C @ 10 secs<br> |
| - | Annealing 60°C 10 secs<br> | + | Annealing - 60°C @ 10 secs<br> |
| - | Extension 72°C 2 mins<br> | + | Extension - 72°C @ 2 mins<br> |
| - | Final extension 72°C 10 mins<br> | + | Final extension - 72°C @ 10 mins<br> |
Revision as of 06:50, 13 September 2013
Polymerase Chain Reaction
PCR mixture
4.0ul 5x phusion buffer
0.4ul dNTPs
0.6ul DMSO
0.2ul Polymerase
11.8ul water
Total 17ul
1ul forward primer
1ul reverse primer
1ul template
PCR settings
Initial denaturation 98°C for 30 secs
Followed by 30 repeats of:
Denaturation - 98 °C @ 10 secs
Annealing - 60°C @ 10 secs
Extension - 72°C @ 2 mins
Final extension - 72°C @ 10 mins
