Team:TU-Munich/Results/Recombinant

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==Investigated Proteins==
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==Characterization of recombinant effector proteins==
Text. See [2013.igem.org/Team:TU-Munich/Notebook/Methods proteinbiochemical methods] for further informatons.
Text. See [2013.igem.org/Team:TU-Munich/Notebook/Methods proteinbiochemical methods] for further informatons.
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Revision as of 14:28, 14 September 2013


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Characterization of recombinant effector proteins

Text. See [2013.igem.org/Team:TU-Munich/Notebook/Methods proteinbiochemical methods] for further informatons.

Table 1:

Investigated Proteins

Protein BioBrick RFC Affinity tag Size [kDa] Disulphid bridges comment
Eryhtromycin Esterase <partinfo>BBa_E0040</partinfo> RFC25 c-term. Streptag II
Laccase <partinfo>BBa_E0040</partinfo> RFC25 c-term. Streptag II
Nano Luciferase <partinfo>BBa_E0040</partinfo> RFC25 c-term. Streptag II
XylE


Eryhtromycin Esterase

[...] description [...] reaction [...] production [...] Characterization

Laccase

[...] description [...] reaction [...] production [...] Characterization

Nano Luciferase

[...] description [...] reaction [...] production [...] Characterization



XylE

[...] description [...] reaction [...] production [...] Characterization





References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.
  2. http://udel.edu/~gshriver/pdf/Pimenteletal1997.pdf Pmentel et al., 1997 Pimentel, D., Wilson, C., McCullum, C., Huang, R., Dwen, P., Flack, J. Tran, Q., Saltman, T., Cliff, T. (1997). Economic and environmental benefits of biodiversity. BioScience, Vol. 47, No. 11., pp. 747-757.



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