Team:TU-Munich/Results/Recombinant

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Characterization of recombinant effector proteins

Text. See [2013.igem.org/Team:TU-Munich/Notebook/Methods proteinbiochemical methods] for further informatons.

Table 1: Investigated Proteins
Protein	BioBrick	RFC	Affinity tag	Size [kDa]	Disulphid bridges	comment
Eryhtromycin Esterase	<partinfo>BBa_E0040</partinfo>	RFC25	c-term. Streptag II
Laccase	<partinfo>BBa_E0040</partinfo>	RFC25	c-term. Streptag II
Nano Luciferase	<partinfo>BBa_E0040</partinfo>	RFC25	c-term. Streptag II
XylE

Eryhtromycin Esterase

File:TUM13 Animation EreB.gif

Figure 3:

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization

Laccase

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization

Nano Luciferase

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization

XylE

File:TUM13 Animation XylE.gif

Figure 3:

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization

SpyCatcher & SpyTag

File:TUM13 Animation Spy.tif

Figure 3:

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization

References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.
http://udel.edu/~gshriver/pdf/Pimenteletal1997.pdf Pmentel et al., 1997 Pimentel, D., Wilson, C., McCullum, C., Huang, R., Dwen, P., Flack, J. Tran, Q., Saltman, T., Cliff, T. (1997). Economic and environmental benefits of biodiversity. BioScience, Vol. 47, No. 11., pp. 747-757.

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