Revision as of 04:55, 16 September 2013

Rehydration of Registry DNA

Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used
Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want
Add 10 μL of ddH₂O (deionized water)
Let the water incubate at room temperature for 10 minutes
Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies

@@ Line 6: / Line 6: @@
 <h2>Protocol</h2>
 <ol>
-<li>Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.</li>
+<li>Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used</li>
-<li>Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want;</li>
+<li>Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li>
-<li>Add 10 μL of ddH<sub>2</sub>O (deionized water);</li>
+<li>Add 10 μL of ddH<sub>2</sub>O (deionized water)</li>
-<li>Let the water incubate at room temperature for 10 minutes;</li>
+<li>Let the water incubate at room temperature for 10 minutes</li>
-<li>Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.</li>
+<li>Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies</li>
 </ol>
 </section>
 </html>