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Team:Calgary/Sandbox/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples
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| + | <li>Culture and induce protein-producing bacteria</li> | ||
<li>Centrifuge cells at max for 10 minutes. Discard supernatant</li> | <li>Centrifuge cells at max for 10 minutes. Discard supernatant</li> | ||
<li>Resuspend cells in 2mL of lysis buffer</li> | <li>Resuspend cells in 2mL of lysis buffer</li> | ||
Latest revision as of 23:31, 22 September 2013
Glass Beads Cell Lysis Protocol for Protein Samples
Reagents and Materials
- 0.1m diameter glass beads
- Lysis Buffer
- Overnight induced cultures
- 1.5mL tubes
Protocol
- Culture and induce protein-producing bacteria
- Centrifuge cells at max for 10 minutes. Discard supernatant
- Resuspend cells in 2mL of lysis buffer
- In a 1.5mL tube, add ~0.5mL of glass beads and 1mL of cells resuspended in lysis buffer
- Leave on ice for 5 min. Leave on BeadBeater or Vortex for 5 min. Repeat this cycle 6 times
- Centrifuge at max for 20 minutes
- Transfer supernatant (crude lysate) to a new tube and discard the glass beads
